Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1299499–1300697	1300697	1–1199	62 [0]	[0] 62	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1299498	0 (0.000)	60 (1.270)	49/282	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_pae_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299351‑1299644                                                                                                                                                    |                                                                                                                                                   ttctCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGATTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTTAAATGATATTATTAATTCAACTGCCTTTGGTAGCGGAAAGTGCTAAa                                                                                                                                                   >  2:25047‑M1/1‑148 (MQ=255)    tcAAAATACAGTTAGTTATAAGGATTTCCTTAACTGCTACTCCTAACCATCAAATTATATTCGCCACATCATAATCCTGGGCATGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                                <  2:32670‑M1/149‑5 (MQ=255)       aaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                             <  2:699265‑M1/149‑8 (MQ=255)             agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                       <  1:710750‑M1/149‑14 (MQ=255)                   ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                                 <  2:482227‑M1/149‑20 (MQ=255)                     ataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGATGGAGAATAAGTTAAATGATATTATTAATTCCACTGCCTTTGGTAGTGAAAAGTGCTAAataataatcaattgttaaatt                                                                                                                               >  2:519237‑M1/1‑128 (MQ=255)                          gATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                                                                                                                          >  1:599396‑M1/1‑123 (MQ=255)                            tttCCTTAACTGCTTCTCCTCACCATCAAGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCAGTGCCTTTGGTAGAGGAAAGTGCTAAataatactaaattcttaacatattattc                                                                                                                        >  2:345681‑M1/1‑121 (MQ=255)                              tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataataaattgttaaattattgtgcat                                                                                                                      >  2:333628‑M1/1‑119 (MQ=255)                                cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAAAGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                    <  1:117615‑M1/149‑33 (MQ=255)                                    aCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcact                                                                                                                >  1:569309‑M1/1‑113 (MQ=255)                                       gCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact                                                                                                             <  1:60352‑M1/149‑40 (MQ=255)                                                       ctgTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaa                                                                                             <  2:821035‑M1/148‑56 (MQ=255)                                                       atGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaa                                                                                             >  2:388306‑M1/1‑94 (MQ=255)                                                            aTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                        <  1:86152‑M1/149‑61 (MQ=255)                                                                   ccccacaataatcctgTGCTCGCTAAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                                                                 <  2:164412‑M1/142‑68 (MQ=255)                                                                   ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                                                                 >  1:217280‑M1/1‑82 (MQ=255)                                                                   ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                                                                 >  1:595092‑M1/1‑82 (MQ=255)                                                                   ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattaatctgctgttcactactggaactgtaatcagaaaagatagacatg                                                                                 <  1:416172‑M1/149‑68 (MQ=255)                                                                    cagagcataatccTGGGCTTGCTGAAGAATAATTGAAATGATATTATGAATGCCACTGACTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcggtactggtactgtgatcagaaaagatagacatgc                                                                                <  2:191904‑M1/144‑69 (MQ=255)                                                                      catcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctt                                                                              >  1:5077‑M1/1‑79 (MQ=255)                                                                        taataaGCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatggttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                            <  2:393774‑M1/147‑73 (MQ=255)                                                                          atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatttttaaattattgtgcatttcactactggaactgtaatcagaaacgatagaaatgcttagcc                                                                          >  1:402747‑M1/1‑75 (MQ=255)                                                                          atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcc                                                                          >  1:650435‑M1/1‑75 (MQ=255)                                                                              tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatc                                                                      >  1:788472‑M1/1‑71 (MQ=255)                                                                                     cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt                                                               <  2:650435‑M1/149‑86 (MQ=255)                                                                                         cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                           <  1:653028‑M1/149‑90 (MQ=255)                                                                                         cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                           >  2:351272‑M1/1‑60 (MQ=255)                                                                                         cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                           <  1:384779‑M1/149‑90 (MQ=255)                                                                                           gaagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattga                                                         >  1:296666‑M1/1‑58 (MQ=255)                                                                                            aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                    <  2:208278‑M1/89‑33 (MQ=255)                                                                                            aagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                    >  1:208278‑M1/1‑57 (MQ=255)                                                                                              gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataatactcaattgttaaattattgtgcatttcactactggaactgtaatcagcaaagatagacatgcttctccactctctatttaattcaatt                                                      >  2:457778‑M1/1‑55 (MQ=255)                                                                                              gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                      <  2:217280‑M1/149‑95 (MQ=255)                                                                                              gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                      <  1:211948‑M1/149‑95 (MQ=255)                                                                                               aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattg                                                     >  2:427334‑M1/1‑54 (MQ=255)                                                                                               aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatc                                                                      >  1:442223‑M1/1‑54 (MQ=255)                                                                                               aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatc                                                                      <  2:442223‑M1/132‑79 (MQ=255)                                                                                                 taatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                           >  2:658432‑M1/1‑52 (MQ=255)                                                                                                 taatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                           <  1:658432‑M1/141‑90 (MQ=255)                                                                                                  aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                                  <  2:361309‑M1/149‑99 (MQ=255)                                                                                                    ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaaga                                                >  2:820824‑M1/1‑49 (MQ=255)                                                                                                    ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaaga                                                <  1:641127‑M1/149‑101 (MQ=255)                                                                                                         aTGATATTAATAATTCCACTGCCTTTGTTAGAGGAAAGTCCTAAataataatcagttgttacattattgttcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagacgttt                                           <  2:645359‑M1/149‑106 (MQ=255)                                                                                                           gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                         <  1:190581‑M1/149‑108 (MQ=255)                                                                                                           gATATTAATAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattagtgtgcatttcactactggaactgtaatcaaaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                         <  2:683152‑M1/149‑108 (MQ=255)                                                                                                              attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                      <  1:334764‑M1/149‑111 (MQ=255)                                                                                                              attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                      <  1:388306‑M1/149‑111 (MQ=255)                                                                                                               ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                     >  1:368353‑M1/1‑38 (MQ=255)                                                                                                               ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                     >  2:495966‑M1/1‑38 (MQ=255)                                                                                                               ttattaATTCCACTGCCTTTGGTAGAGAAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                     <  1:848357‑M1/149‑112 (MQ=255)                                                                                                                       tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctctttgattaaattgaaagatgtttgttaaggcatggat                             >  2:281494‑M1/1‑30 (MQ=255)                                                                                                                           cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                         <  2:488744‑M1/149‑124 (MQ=255)                                                                                                                            tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaatttattgtgtatttcactactggaactgtaatcataaaagattgacatgcttagcaaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                        <  1:407909‑M1/149‑125 (MQ=255)                                                                                                                             gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                       <  2:599396‑M1/149‑126 (MQ=255)                                                                                                                                  tGGTATAGGAAGTTGCTAAataataattaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatag                  <  2:361126‑M1/149‑131 (MQ=255)                                                                                                                                   ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctataga                 <  1:333628‑M1/149‑132 (MQ=255)                                                                                                                                    gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagat                >  2:131532‑M1/1‑17 (MQ=255)                                                                                                                                     tAGAGGAAAGTTCTAAacaataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagatt               <  1:414813‑M1/149‑134 (MQ=255)                                                                                                                                       gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaataagaaaagatagacatgcttagccaatctctatttgattgaattgatagatgtttgttaaggcatggatgcaagctatcggttct             >  2:366895‑M1/1‑14 (MQ=255)                                                                                                                                                 cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtca   >  2:563560‑M1/1‑4 (MQ=255)                                                                                                                                                  tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgtatgccagctatagattctgatacggtcaa  >  2:331618‑M1/1‑3 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299351‑1299644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑