breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 264,098 | G→T | L50L (CTC→CTA) | ykfH ← | uncharacterized protein |
RA | 275,065 | G→A | T18I (ACC→ATC) | insH1 ← | IS5 transposase and trans‑activator |
RA | 482,230 | G→A | P725L (CCG→CTG) | acrB ← | multidrug efflux system protein |
RA | 1,197,502 | C→T | G11R (GGA→AGA) | ymfD ← | e14 prophage; putative SAM‑dependent methyltransferase |
RA | 1,263,669 | G→A | intergenic (‑169/‑45) | lolB ← / → hemA | lipoprotein localization factor/glutamyl tRNA reductase |
RA | 1,544,022 | 1 bp→AA | coding (39/1389 nt) | narU ← | nitrate/nitrite transporter |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,106,741 | A→T | S162S (TCT→TCA) | wbbH ← | O‑antigen polymerase |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,679,525 | G→T | A1074D (GCT→GAT) | yphG ← | DUF4380 domain‑containing TPR repeat protein |
RA | 2,795,396 | C→A | Q382K (CAG→AAG) | gabP → | gamma‑aminobutyrate transporter |
RA | 2,817,170 | C→A | E112* (GAA→TAA) | yqaB ← | fructose‑1‑P and 6‑phosphogluconate phosphatase |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 4,110,747 | G→T | intergenic (+62/+784) | cdh → / ← yiiQ | CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein |
RA | 4,296,297 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,604,632 | C→A | A186E (GCA→GAA) | bglJ → | bgl operon transcriptional activator |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913_3_pae_tpiA | 3423748–3424234 | 3424578–3424238 | 5–831 | 42 [39] | [39] 41 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913_3_pae_tpiA | = 1050546 | 80 (0.860) | 47 (0.510) | 44/248 | 2.0 | 49.9% | intergenic (+16/+417) | insB1/cspH | IS1 transposase B/stress protein, member of the CspA‑family |
? | NC_000913_3_pae_tpiA | = 2490626 | 15 (0.160) | coding (1325/1695 nt) | oxc | oxalyl CoA decarboxylase, ThDP‑dependent | |||||
* | ? | NC_000913_3_pae_tpiA | 1207790 = | 24 (0.260) | 41 (0.510) | 37/218 | 2.1 | 61.7% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913_3_pae_tpiA | 1209619 = | 30 (0.370) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913_3_pae_tpiA | = 1207805 | 24 (0.260) | 44 (0.540) | 36/218 | 2.2 | 63.3% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913_3_pae_tpiA | = 1209602 | 30 (0.370) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913_3_pae_tpiA | = 1299498 | 0 (0.000) | 95 (1.050) | 72/242 | 0.2 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913_3_pae_tpiA | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913_3_pae_tpiA | 1467910 = | NA (NA) | 114 (1.220) | 81/250 | 0.1 | 90.5% | intergenic (+6/+11) | ydbA/insD1 | pseudogene, autotransporter homolog; interrupted by IS2 and IS30/IS2 transposase TnpB |
? | NC_000913_3_pae_tpiA | = 1755832 | 12 (0.130) | coding (135/1413 nt) | pykF | pyruvate kinase I | |||||
* | ? | NC_000913_3_pae_tpiA | = 1469240 | NA (NA) | 94 (1.010) | 68/250 | 0.4 | 80.3% | intergenic (‑91/‑1) | insC1/ydbA | IS2 repressor TnpA/pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
? | NC_000913_3_pae_tpiA | 1755828 = | 23 (0.250) | coding (131/1413 nt) | pykF | pyruvate kinase I | |||||
* | ? | NC_000913_3_pae_tpiA | = 1979269 | NA (NA) | 80 (0.870) | 73/248 | 0.2 | 82.6% | intergenic (‑54/‑484) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913_3_pae_tpiA | 2490618 = | 17 (0.180) | coding (1333/1695 nt) | oxc | oxalyl CoA decarboxylase, ThDP‑dependent |