breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 275,065 G→A T18I (ACC→ATC)  insH1 ← IS5 transposase and trans‑activator
RA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,263,669 G→A intergenic (‑169/‑45) lolB ← / → hemA lipoprotein localization factor/glutamyl tRNA reductase
RA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,106,741 A→T S162S (TCT→TCA wbbH ← O‑antigen polymerase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,795,396 C→A Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter
RA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,110,747 G→T intergenic (+62/+784) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
RA 4,296,297 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,604,632 C→A A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 3423748–3424234 3424578–3424238 5–831 42 [39] [39] 41 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 105054680 (0.860)47 (0.510) 44/248 2.0 49.9% intergenic (+16/+417) insB1/cspH IS1 transposase B/stress protein, member of the CspA‑family
?NC_000913_3_pae_tpiA = 2490626 15 (0.160)coding (1325/1695 nt) oxc oxalyl CoA decarboxylase, ThDP‑dependent
* ? NC_000913_3_pae_tpiA 1207790 =24 (0.260)41 (0.510) 37/218 2.1 61.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_pae_tpiA 1209619 = 30 (0.370)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_pae_tpiA = 120780524 (0.260)44 (0.540) 36/218 2.2 63.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913_3_pae_tpiA = 1209602 30 (0.370)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)95 (1.050) 72/242 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913_3_pae_tpiA 1467910 =NA (NA)114 (1.220) 81/250 0.1 90.5% intergenic (+6/+11) ydbA/insD1 pseudogene, autotransporter homolog; interrupted by IS2 and IS30/IS2 transposase TnpB
?NC_000913_3_pae_tpiA = 1755832 12 (0.130)coding (135/1413 nt) pykF pyruvate kinase I
* ? NC_000913_3_pae_tpiA = 1469240NA (NA)94 (1.010) 68/250 0.4 80.3% intergenic (‑91/‑1) insC1/ydbA IS2 repressor TnpA/pseudogene, autotransporter homolog; interrupted by IS2 and IS30
?NC_000913_3_pae_tpiA 1755828 = 23 (0.250)coding (131/1413 nt) pykF pyruvate kinase I
* ? NC_000913_3_pae_tpiA = 1979269NA (NA)80 (0.870) 73/248 0.2 82.6% intergenic (‑54/‑484) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913_3_pae_tpiA 2490618 = 17 (0.180)coding (1333/1695 nt) oxc oxalyl CoA decarboxylase, ThDP‑dependent