New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 4150329	63 (1.800)	5 (0.150)	5/266	3.9	7.7%	intergenic (‑148/+34)	eptC/ppc	LPS heptose I phosphoethanolamine transferase/phosphoenolpyruvate carboxylase
	?	NC_000913_3_pae_tpiA	= 4150339	61 (1.860)					intergenic (‑158/+24)	eptC/ppc	LPS heptose I phosphoethanolamine transferase/phosphoenolpyruvate carboxylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4150175‑4150329 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaaccctcgTGCTTTTGCGCGAGGGTTTTCTGAAATACTTCTGTTCTAACACCCTCGTTTTCAATATATTTCTGTCTGCATTTTATTCAAATTCTGAATATACCTTCAGATATCCTTAAGGAATTGTCGTTACATTCGGCGATATTTTTTCAA  <  NC_000913_3_pae_tpiA/4150339‑4150196                                                                                                                                                                                                                                                                                                             AATGTATAGTAAGAACCTGGCATGAAAAAATATCGCCGAATGTAACGACAATTGCTGAAGGATATCTGAAGGTATATTCAGAATTTGAAGAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAA                                                                                                                                                        <  2:63048/149‑1      ATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGACATATATTGAAAACGAAGGTGTTAGAACCAAAGTAATAAAGACAACCCTC                                                                                                                                                   >  2:328952/1‑149      ATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGACAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTC                                                                                                                                                   >  2:510074/1‑149                 CTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGTGCTTTTGCG                                                                                                                                        >  2:570316/1‑149                     CTTGAAAAAATATCGCCGAATGTACGTACAATTCCTTAAGGATATCTGAAGTTATATTCAGAATTTGAATAAAATGCAGACCGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGTGCTTTTGCGCGAG                                                                                                                                    <  2:482754/149‑1                      TTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGTGCTTTTGCGCGAGG                                                                                                                                   <  1:570316/149‑1                                                                   TGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGTGCTTTTGCGCGAGGGTTTTCTGAAATAC                                                                                                                     <  1:30481/118‑1                                                                   TGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCGTGCTTTTGCGCGAGGGTTTTCTGAAATAC                                                                                                                     >  2:30481/1‑118                                                                                         ATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAAC                                                                                                                                                       <  1:31390/62‑1                                                                                         ATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAAC                                                                                                                                                       >  2:31390/1‑62                                                                                                                                                       CCTCGTGCTTTTGCGCGAGGGTTTTCTGAAATACTTCTGTTCTAACACCCTCGTTTTCAATATATTTCTGTCTGCATTTTATTCAAATTCTGAATATACCTTCAGATATCCTTAAGGAATTGTCGTTACATTCGGCGATATTTTTTCAA  <  1:108084/149‑1                                                                                                                                                       CCTCGTGCTTTTGCGCGAGGGTTTTCTGAAATACTTCTGTTCTAACACCCTCGTTTTCAATATATTTCTGTCTGCATTTTATTCAAATTCTGAATATACCTTCAGATATCCTTAAGGAATTGTCGTTACATTCGGCGATATTTTTTCAA  >  2:316164/1‑149                                                                                                                                                                                                                                                                                                             AATGCATAGTAAGAACCTGTCTTGAAAAAATATCGCCGAATGTAACGACAATTCCTTAAGGATATCTGAAGGTATATTCAGAATTTGAATAAAATGCAGACAGAAATATATTGAAAACGAGGGTGTTAGAACAGAAGTATTTCAGAAAACCCTCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4150175‑4150329 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaaccctcgTGCTTTTGCGCGAGGGTTTTCTGAAATACTTCTGTTCTAACACCCTCGTTTTCAATATATTTCTGTCTGCATTTTATTCAAATTCTGAATATACCTTCAGATATCCTTAAGGAATTGTCGTTACATTCGGCGATATTTTTTCAA  <  NC_000913_3_pae_tpiA/4150339‑4150196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.