Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,483,617 Δ5 bp coding (557‑561/3903 nt) hrpA → putative ATP‑dependent helicase

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1483617 1483621 5 24 [0] [0] 24 hrpA putative ATP‑dependent helicase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 14836160 (0.000)24 (0.660) 22/282 0.8 100% coding (556/3903 nt) hrpA putative ATP‑dependent helicase
?NC_000913_3_pae_tpiA 1483622 = 0 (0.000)coding (562/3903 nt) hrpA putative ATP‑dependent helicase

AACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGA  >  NC_000913_3_pae_tpiA/1483478‑1483758
                                                                                                                                                |                                                                                                                                        
tggcgaaccgtattgcggaagagctgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGAt                                                                                                                                      >  1:597666‑M2/145‑149 (MQ=255)
    gaaccgtattgcggaagagctgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAg                                                                                                                                  <  2:549881‑M2/9‑1 (MQ=255)
       ccgtattgcggaagagctgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTAc                                                                                                                               >  1:405493‑M2/138‑149 (MQ=255)
                      gctgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgctcacgaaagtgcacaaacgatgtacaacctgataaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCattatt                                                                                                                <  2:547329‑M2/27‑1 (MQ=255)
                        tgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGa                                                                                                              >  1:391995‑M2/121‑149 (MQ=255)
                        tgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGa                                                                                                              >  1:616701‑M2/121‑149 (MQ=255)
                        tgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGa                                                                                                              <  2:326413‑M2/29‑1 (MQ=255)
                        gcaagacggagccgggcggttacatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGa                                                                                                              <  2:30607‑M2/29‑1 (MQ=255)
                                            tgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGcc                                                                                          <  2:405493‑M2/49‑1 (MQ=255)
                                               atcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGa                                                                                       >  1:101709‑M2/98‑149 (MQ=255)
                                                tcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGGGCACGAACGCAGCCTGaa                                                                                      >  1:295357‑M2/97‑149 (MQ=255)
                                                    ttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATc                                                                                  <  1:202788‑M2/57‑1 (MQ=255)
                                                                               aagtgataacctggtggtcatgctgatcaccgacggtatcctgcaggcggagatccagcaagaccCTGATGCAGTACTACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAagag                                                       <  1:362663‑M2/84‑1 (MQ=255)
                                                                                  tgagaacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTtg                                                    <  1:603117‑M2/87‑1 (MQ=255)
                                                                                         acgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCcgc                                             >  2:89199‑M2/56‑149 (MQ=255)
                                                                                          cgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaaggccCTGATGCAGTACGAGGCTATGATTATTGACGAAGCGCACGAACGCGGCCTGAATATCGATTTTTTGCTCGGCTTTTTGGAGGAGTTGCTgccgag                                            >  2:149427‑M2/55‑147 (MQ=255)
                                                                                               gtcaagaggatgacggacggtatcctgctgccggagatccagcaagcccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCAGGAACGCAGCTTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTc                                       <  2:493170‑M2/100‑1 (MQ=255)
                                                                                                tcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTcc                                      >  1:387724‑M2/49‑149 (MQ=255)
                                                                                                        atgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCCGTACGACACTATCATTATTGACGAAGGGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTaa                              >  1:256743‑M2/41‑149 (MQ=255)
                                                                                                                 ggtatcctgctggcggagatccagcaagaccGTGATGCAGTAGGACACTATGATTATTGACGAGGCGCACGAACGCAGCCTGGATATCGATTTTTTGCTAGGCTTTTTGAAAGAGTTGGTGCAGCGGCGTTCTGACCTTAAAATCATTa                     >  2:28487‑M2/32‑149 (MQ=255)
                                                                                                                           tggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCcgc           >  1:398094‑M2/22‑149 (MQ=255)
                                                                                                                               ggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACt       >  2:474631‑M2/18‑149 (MQ=255)
                                                                                                                                  gatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATc    <  2:391995‑M2/135‑1 (MQ=255)
                                                                                                                                    tccagcaagaccCTGATGCAGTACGACACTATCATTATTGTCGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGa  <  2:231893‑M2/137‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                        
AACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGA  >  NC_000913_3_pae_tpiA/1483478‑1483758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.