Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,483,617	Δ5 bp	coding (557‑561/3903 nt)	hrpA →	putative ATP‑dependent helicase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1483617	1483621	5	24 [0]	[0] 24	hrpA	putative ATP‑dependent helicase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1483616	0 (0.000)	24 (0.660)	22/282	0.8	100%	coding (556/3903 nt)	hrpA	putative ATP‑dependent helicase
	?	NC_000913_3_pae_tpiA	1483622 =	0 (0.000)					coding (562/3903 nt)	hrpA	putative ATP‑dependent helicase

AACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGA  >  NC_000913_3_pae_tpiA/1483478‑1483758                                                                                                                                                 |                                                                                                                                         tggcgaaccgtattgcggaagagctgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGAt                                                                                                                                      >  1:597666‑M2/145‑149 (MQ=255)     gaaccgtattgcggaagagctgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAg                                                                                                                                  <  2:549881‑M2/9‑1 (MQ=255)        ccgtattgcggaagagctgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTAc                                                                                                                               >  1:405493‑M2/138‑149 (MQ=255)                       gctgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgctcacgaaagtgcacaaacgatgtacaacctgataaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCattatt                                                                                                                <  2:547329‑M2/27‑1 (MQ=255)                         tgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGa                                                                                                              >  1:391995‑M2/121‑149 (MQ=255)                         tgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGa                                                                                                              >  1:616701‑M2/121‑149 (MQ=255)                         tgaaaacggagccgggcggttgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGa                                                                                                              <  2:326413‑M2/29‑1 (MQ=255)                         gcaagacggagccgggcggttacatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGa                                                                                                              <  2:30607‑M2/29‑1 (MQ=255)                                             tgcatcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGcc                                                                                          <  2:405493‑M2/49‑1 (MQ=255)                                                atcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGa                                                                                       >  1:101709‑M2/98‑149 (MQ=255)                                                 tcggttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGGGCACGAACGCAGCCTGaa                                                                                      >  1:295357‑M2/97‑149 (MQ=255)                                                     ttacaaagtgcgtttcagcgatcacgtaagtgataacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATc                                                                                  <  1:202788‑M2/57‑1 (MQ=255)                                                                                aagtgataacctggtggtcatgctgatcaccgacggtatcctgcaggcggagatccagcaagaccCTGATGCAGTACTACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAagag                                                       <  1:362663‑M2/84‑1 (MQ=255)                                                                                   tgagaacacgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTtg                                                    <  1:603117‑M2/87‑1 (MQ=255)                                                                                          acgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCcgc                                             >  2:89199‑M2/56‑149 (MQ=255)                                                                                           cgatggtcaagctgatgaccgacggtatcctgctggcggagatccagcaaggccCTGATGCAGTACGAGGCTATGATTATTGACGAAGCGCACGAACGCGGCCTGAATATCGATTTTTTGCTCGGCTTTTTGGAGGAGTTGCTgccgag                                            >  2:149427‑M2/55‑147 (MQ=255)                                                                                                gtcaagaggatgacggacggtatcctgctgccggagatccagcaagcccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCAGGAACGCAGCTTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTc                                       <  2:493170‑M2/100‑1 (MQ=255)                                                                                                 tcaagctgatgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTcc                                      >  1:387724‑M2/49‑149 (MQ=255)                                                                                                         atgaccgacggtatcctgctggcggagatccagcaagaccCTGATGCCGTACGACACTATCATTATTGACGAAGGGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTaa                              >  1:256743‑M2/41‑149 (MQ=255)                                                                                                                  ggtatcctgctggcggagatccagcaagaccGTGATGCAGTAGGACACTATGATTATTGACGAGGCGCACGAACGCAGCCTGGATATCGATTTTTTGCTAGGCTTTTTGAAAGAGTTGGTGCAGCGGCGTTCTGACCTTAAAATCATTa                     >  2:28487‑M2/32‑149 (MQ=255)                                                                                                                            tggcggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCcgc           >  1:398094‑M2/22‑149 (MQ=255)                                                                                                                                ggagatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACt       >  2:474631‑M2/18‑149 (MQ=255)                                                                                                                                   gatccagcaagaccCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATc    <  2:391995‑M2/135‑1 (MQ=255)                                                                                                                                     tccagcaagaccCTGATGCAGTACGACACTATCATTATTGTCGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGa  <  2:231893‑M2/137‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                         AACCGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTCAGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCGGAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGA  >  NC_000913_3_pae_tpiA/1483478‑1483758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑