Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300697 1300697 1–1199 51 [0] [0] 51 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)45 (0.840) 41/276 0.3 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299354‑1299644
                                                                                                                                                |                                                                                                                                                  
tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAAAGGAAAATGCTAAataa                                                                                                                                                >  2:575544‑M1/1‑145 (MQ=255)
   aaatacaGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATGAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                             <  2:360513‑M1/146‑8 (MQ=255)
                  taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATGGAAATGATATTATTAATTCAACTGCATTTGGTAGAGGAAAGTGCTAAataataataaattgttagatta                                                                                                                              >  2:17179‑M1/1‑127 (MQ=255)
                   aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                                                                                                                             >  1:907776‑M1/1‑126 (MQ=255)
                    aGGTTTTCGTTAACTGCTTCTCCTCACCATTATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGTCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                            <  2:442539‑M1/149‑25 (MQ=255)
                          tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTGGCCATATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAttaataatcaattgttaatttattttgctt                                                                                                                      >  2:706266‑M1/1‑119 (MQ=255)
                          tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTTGTAGAGGAAAGTGCTAActagtaatcaatttttaaatttttgttaat                                                                                                                      >  1:175622‑M1/1‑119 (MQ=255)
                            cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaatttattatgcgtgt                                                                                                                    >  2:255450‑M1/1‑117 (MQ=255)
                             ttAACTGCTTCTCCTCACCATCATGGTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTAAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgctaaattattgtgcatttc                                                                                                                   >  1:751124‑M1/1‑116 (MQ=255)
                              tgagtgcttCTCCGAACCAGCATGTTATTTACGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAGATGATATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                                  <  2:344971‑M1/145‑35 (MQ=255)
                                aCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGACTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattctccatttccct                                                                                                                >  2:46740‑M1/1‑113 (MQ=255)
                                  tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac                                                                                                              <  2:649249‑M1/149‑39 (MQ=255)
                                    cTTCACCTCTCCATTATGTTATTTTCGCCACATTATAATCCTGGGTTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                            <  2:798680‑M1/149‑41 (MQ=255)
                                           tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                                     >  1:902296‑M1/1‑102 (MQ=255)
                                                atcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                                <  1:810106‑M1/149‑53 (MQ=255)
                                                atcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                                <  2:240908‑M1/149‑53 (MQ=255)
                                                        aTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                        >  2:79393‑M1/1‑89 (MQ=255)
                                                              gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                  >  1:643424‑M1/1‑83 (MQ=255)
                                                              gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                  >  2:389389‑M1/1‑83 (MQ=255)
                                                               ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggcactgtaatcagaaaagatagacatg                                                                                 >  2:392043‑M1/1‑82 (MQ=255)
                                                                cacaTCACAATCCCTGTCTGGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                                <  2:402708‑M1/149‑69 (MQ=255)
                                                                 acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgct                                                                               >  1:646238‑M1/1‑80 (MQ=255)
                                                                 acaTCATAATCCTGCGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggcactgtaatcagaaaagatagacatgct                                                                               <  2:968306‑M1/149‑70 (MQ=255)
                                                                      atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatggtgcatttcactactggaactgtaatcataaaagatagaaaggtttagcc                                                                          >  1:976735‑M1/1‑75 (MQ=255)
                                                                        aaTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaa                                                                        >  2:273038‑M1/1‑73 (MQ=255)
                                                                            cTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccactctc                                                                    >  2:544428‑M1/1‑69 (MQ=255)
                                                                            cTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctc                                                                    <  1:389389‑M1/149‑81 (MQ=255)
                                                                                 cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttcgcaaatctctattt                                                               >  1:120657‑M1/1‑64 (MQ=255)
                                                                                    gCTGAAGAATAATTGAAATGATATTATTAAGTCCACACTGCTTGTTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagctagacatgattagcaaatctctatttgat                                                            <  2:454287‑M1/149‑89 (MQ=255)
                                                                                     cTGAAGAATAATTGGAATGATATTATTAGTTCCACTGCCTGTGGTAGAGGAAAGTGCTAAataataatcaattgttaaactattgtgcatttcactactggacctgtaatcagaacagatagacatgcttagccaatctctatttgatt                                                           <  2:338304‑M1/149‑90 (MQ=255)
                                                                                       gaagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcatttgttaaattattgtgcatttcactactggaacagtagtcagaaaagatagacatgcttagccaatctctatttgattga                                                         >  2:850424‑M1/1‑58 (MQ=255)
                                                                                            ataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattga                                                    <  2:248376‑M1/149‑97 (MQ=255)
                                                                                                ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactcatggaactgtaatcagaaaagatagacatgcttcgccaatctgtatatgatggaattgaaaga                                                >  2:686364‑M1/1‑49 (MQ=255)
                                                                                                    aaTGATATTAATAATTCCACTGCCTTTCGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcagttctctactgtaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtt                                            <  2:727154‑M1/149‑105 (MQ=255)
                                                                                                          attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                      <  2:643424‑M1/149‑111 (MQ=255)
                                                                                                                   tacacTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                             <  2:327731‑M1/147‑120 (MQ=255)
                                                                                                                            tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                    >  2:316481‑M1/1‑21 (MQ=255)
                                                                                                                            tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                           >  2:502231‑M1/1‑21 (MQ=255)
                                                                                                                            tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                           <  1:502231‑M1/126‑106 (MQ=255)
                                                                                                                              tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactagtggaactgtaatcagaaaagatagacatgcttagacaatccctagttgattgaattgaaagatgtttgttaaggcatggatgcgagataaag                  >  2:647499‑M1/1‑19 (MQ=255)
                                                                                                                                  agagGGAAGTGCTAAataataatcgattgtttaattattgggtattgcactactggaactgtaatcagaaaaggtagcaatgcttagcgaagctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc              <  2:410848‑M1/149‑135 (MQ=255)
                                                                                                                                    agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctg            >  1:583276‑M1/1‑13 (MQ=255)
                                                                                                                                         aGTGCCAAataataagcaattgtgaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg       <  2:372820‑M1/149‑142 (MQ=255)
                                                                                                                                          gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacgg      <  1:708314‑M1/149‑143 (MQ=255)
                                                                                                                                           tGCTAAataataatcaattgttaaattattgtgcatttaactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt     <  2:515228‑M1/149‑144 (MQ=255)
                                                                                                                                             cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata                   >  1:758561‑M1/1‑4 (MQ=255)
                                                                                                                                             cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata                   <  2:758561‑M1/133‑130 (MQ=255)
                                                                                                                                              tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcataaaagatagacatgcttagctaatctctatttgatcgaattaaatgatgtttgttaatgaatggttgcaagctaattattctgataccgtcaa  >  2:464874‑M1/1‑3 (MQ=255)
                                                                                                                                              tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaa  <  1:414129‑M1/149‑147 (MQ=255)
                                                                                                                                              tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                               >  1:162572‑M1/1‑3 (MQ=255)
                                                                                                                                              tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                               <  2:162572‑M1/104‑102 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299354‑1299644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.