Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300697 1300697 1–1199 51 [0] [0] 51 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)45 (0.840) 41/276 0.3 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300553‑1300843
                                                                                                                                                 |                                                                                                                                                 
tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtaaaggaaaatgctaaataa                                                                                                                                                >  2:575544‑M2/146‑149 (MQ=255)
   aaatacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattatgaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                             <  2:360513‑M2/7‑1 (MQ=255)
                  taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataatggaaatgatattattaattcaactgcatttggtagaggaaagtgctaaATAATAATAAATTGTTAGatta                                                                                                                              >  2:17179‑M2/128‑149 (MQ=255)
                   aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                             >  1:907776‑M2/127‑149 (MQ=255)
                    aggttttcgttaactgcttctcctcaccattatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgtctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                            <  2:442539‑M2/24‑1 (MQ=255)
                          tccttaactgcttctcctcaccatcatgttattttggccatatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaTTAATAATCAATTGTTAATTTATTTtgctt                                                                                                                      >  2:706266‑M2/120‑147 (MQ=255)
                          tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgccttttgtagaggaaagtgctaaCTAGTAATCAATTTTTAAAtttttgttaat                                                                                                                      >  1:175622‑M2/120‑145 (MQ=255)
                            cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAATTTATtatgcgtgt                                                                                                                    >  2:255450‑M2/118‑145 (MQ=255)
                             ttaactgcttctcctcaccatcatggtattttcgccacatcataatcctgggcttgctgaagaataattaaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGCTAAATTATTGTGCATTTc                                                                                                                   >  1:751124‑M2/117‑149 (MQ=255)
                              tgagtgcttctccgaaccagcatgttatttacgccacatcataatcctgggcttgctgaagaataattgagatgatattattaattccactgcttttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                                  <  2:344971‑M2/34‑1 (MQ=255)
                                actgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagactaattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTCTCCATTTCcct                                                                                                                >  2:46740‑M2/114‑149 (MQ=255)
                                  tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactac                                                                                                              <  2:649249‑M2/38‑1 (MQ=255)
                                    cttcacctctccattatgttattttcgccacattataatcctgggtttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                            <  2:798680‑M2/40‑1 (MQ=255)
                                           tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGt                                                                                                     >  1:902296‑M2/103‑149 (MQ=255)
                                                atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                                <  1:810106‑M2/52‑1 (MQ=255)
                                                atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                                <  2:240908‑M2/52‑1 (MQ=255)
                                                        attttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                        >  2:79393‑M2/90‑149 (MQ=255)
                                                              gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                                  >  1:643424‑M2/84‑149 (MQ=255)
                                                              gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                                  >  2:389389‑M2/84‑149 (MQ=255)
                                                               ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGCACTGTAATCAGAAAAGATAGACATg                                                                                 >  2:392043‑M2/83‑149 (MQ=255)
                                                                cacatcacaatccctgtctggctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                                <  2:402708‑M2/68‑1 (MQ=255)
                                                                 acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCt                                                                               >  1:646238‑M2/81‑149 (MQ=255)
                                                                 acatcataatcctgcgcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGCACTGTAATCAGAAAAGATAGACATGCt                                                                               <  2:968306‑M2/69‑1 (MQ=255)
                                                                      ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATGGTGCATTTCACTACTGGAACTGTAATCATAAAAGATAGAAAGGTTTAGcc                                                                          >  1:976735‑M2/76‑149 (MQ=255)
                                                                        aatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCaa                                                                        >  2:273038‑M2/74‑149 (MQ=255)
                                                                            ctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCACtctc                                                                    >  2:544428‑M2/70‑149 (MQ=255)
                                                                            ctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtctc                                                                    <  1:389389‑M2/80‑1 (MQ=255)
                                                                                 cttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTCGCAAATCTCTAttt                                                               >  1:120657‑M2/65‑149 (MQ=255)
                                                                                    gctgaagaataattgaaatgatattattaagtccacactgcttgttagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGCTAGACATGATTAGCAAATCTCTATttgat                                                            <  2:454287‑M2/88‑1 (MQ=255)
                                                                                     ctgaagaataattggaatgatattattagttccactgcctgtggtagaggaaagtgctaaATAATAATCAATTGTTAAACTATTGTGCATTTCACTACTGGACCTGTAATCAGAACAGATAGACATGCTTAGCCAATCTCTATttgatt                                                           <  2:338304‑M2/89‑1 (MQ=255)
                                                                                       gaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCATTTGTTAAATTATTGTGCATTTCACTACTGGAACAGTAGTCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattga                                                         >  2:850424‑M2/59‑149 (MQ=255)
                                                                                            ataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattga                                                    <  2:248376‑M2/96‑1 (MQ=255)
                                                                                                ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTCATGGAACTGTAATCAGAAAAGATAGACATGCTTCGCCAATCTGTATATGATGGAATTGAAAGa                                                >  2:686364‑M2/50‑149 (MQ=255)
                                                                                                    aatgatattaataattccactgcctttcgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAGTTCTCTACTGTAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtt                                            <  2:727154‑M2/104‑1 (MQ=255)
                                                                                                          attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                      <  2:643424‑M2/110‑1 (MQ=255)
                                                                                                                   tacactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                             <  2:327731‑M2/119‑1 (MQ=255)
                                                                                                                            tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                           >  2:502231‑M2/22‑126 (MQ=255)
                                                                                                                            tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                           <  1:502231‑M2/105‑1 (MQ=255)
                                                                                                                            tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                    >  2:316481‑M2/22‑149 (MQ=255)
                                                                                                                              tggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTAGTGGAACTGTAATCAGAAAAGATAGACATGCTTAGACAATCCCTAGTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCGAGATAAAg                  >  2:647499‑M2/20‑149 (MQ=255)
                                                                                                                                  agagggaagtgctaaATAATAATCGATTGTTTAATTATTGGGTATTGCACTACTGGAACTGTAATCAGAAAAGGTAGCAATGCTTAGCGAAGCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc              <  2:410848‑M2/134‑1 (MQ=255)
                                                                                                                                    aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTg            >  1:583276‑M2/14‑149 (MQ=255)
                                                                                                                                         agtgccaaATAATAAGCAATTGTGAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg       <  2:372820‑M2/141‑1 (MQ=255)
                                                                                                                                          gtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACgg      <  1:708314‑M2/142‑1 (MQ=255)
                                                                                                                                           tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTAACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt     <  2:515228‑M2/143‑1 (MQ=255)
                                                                                                                                             ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata                   >  1:758561‑M2/5‑133 (MQ=255)
                                                                                                                                             ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata                   <  2:758561‑M2/129‑1 (MQ=255)
                                                                                                                                              taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCATAAAAGATAGACATGCTTAGCTAATCTCTATTTGATCGAATTAAATGATGTTTGTTAATGAATGGTTGCAAGCTAATTATTCTGATACCGTCaa  >  2:464874‑M2/4‑149 (MQ=255)
                                                                                                                                              taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                               >  1:162572‑M2/4‑104 (MQ=255)
                                                                                                                                              taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                               <  2:162572‑M2/101‑1 (MQ=255)
                                                                                                                                              taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCaa  <  1:414129‑M2/146‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300553‑1300843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.