Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1299499–1300697	1300697	1–1199	51 [0]	[0] 51	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1299498	0 (0.000)	45 (0.840)	41/276	0.3	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_pae_tpiA	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299354‑1299644                                                                                                                                                 |                                                                                                                                                   tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAAAGGAAAATGCTAAataa                                                                                                                                                >  2:575544‑M1/1‑145 (MQ=255)    aaatacaGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATGAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                             <  2:360513‑M1/146‑8 (MQ=255)                   taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATGGAAATGATATTATTAATTCAACTGCATTTGGTAGAGGAAAGTGCTAAataataataaattgttagatta                                                                                                                              >  2:17179‑M1/1‑127 (MQ=255)                    aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattat                                                                                                                             >  1:907776‑M1/1‑126 (MQ=255)                     aGGTTTTCGTTAACTGCTTCTCCTCACCATTATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGTCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                            <  2:442539‑M1/149‑25 (MQ=255)                           tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTGGCCATATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAttaataatcaattgttaatttattttgctt                                                                                                                      >  2:706266‑M1/1‑119 (MQ=255)                           tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTTGTAGAGGAAAGTGCTAActagtaatcaatttttaaatttttgttaat                                                                                                                      >  1:175622‑M1/1‑119 (MQ=255)                             cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaatttattatgcgtgt                                                                                                                    >  2:255450‑M1/1‑117 (MQ=255)                              ttAACTGCTTCTCCTCACCATCATGGTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTAAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgctaaattattgtgcatttc                                                                                                                   >  1:751124‑M1/1‑116 (MQ=255)                               tgagtgcttCTCCGAACCAGCATGTTATTTACGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAGATGATATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                                  <  2:344971‑M1/145‑35 (MQ=255)                                 aCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGACTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattctccatttccct                                                                                                                >  2:46740‑M1/1‑113 (MQ=255)                                   tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac                                                                                                              <  2:649249‑M1/149‑39 (MQ=255)                                     cTTCACCTCTCCATTATGTTATTTTCGCCACATTATAATCCTGGGTTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                            <  2:798680‑M1/149‑41 (MQ=255)                                            tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                                     >  1:902296‑M1/1‑102 (MQ=255)                                                 atcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                                <  1:810106‑M1/149‑53 (MQ=255)                                                 atcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                                <  2:240908‑M1/149‑53 (MQ=255)                                                         aTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagat                                                                                        >  2:79393‑M1/1‑89 (MQ=255)                                                               gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                  >  1:643424‑M1/1‑83 (MQ=255)                                                               gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                  >  2:389389‑M1/1‑83 (MQ=255)                                                                ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggcactgtaatcagaaaagatagacatg                                                                                 >  2:392043‑M1/1‑82 (MQ=255)                                                                 cacaTCACAATCCCTGTCTGGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                                <  2:402708‑M1/149‑69 (MQ=255)                                                                  acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgct                                                                               >  1:646238‑M1/1‑80 (MQ=255)                                                                  acaTCATAATCCTGCGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggcactgtaatcagaaaagatagacatgct                                                                               <  2:968306‑M1/149‑70 (MQ=255)                                                                       atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatggtgcatttcactactggaactgtaatcataaaagatagaaaggtttagcc                                                                          >  1:976735‑M1/1‑75 (MQ=255)                                                                         aaTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaa                                                                        >  2:273038‑M1/1‑73 (MQ=255)                                                                             cTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccactctc                                                                    >  2:544428‑M1/1‑69 (MQ=255)                                                                             cTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctc                                                                    <  1:389389‑M1/149‑81 (MQ=255)                                                                                  cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttcgcaaatctctattt                                                               >  1:120657‑M1/1‑64 (MQ=255)                                                                                     gCTGAAGAATAATTGAAATGATATTATTAAGTCCACACTGCTTGTTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagctagacatgattagcaaatctctatttgat                                                            <  2:454287‑M1/149‑89 (MQ=255)                                                                                      cTGAAGAATAATTGGAATGATATTATTAGTTCCACTGCCTGTGGTAGAGGAAAGTGCTAAataataatcaattgttaaactattgtgcatttcactactggacctgtaatcagaacagatagacatgcttagccaatctctatttgatt                                                           <  2:338304‑M1/149‑90 (MQ=255)                                                                                        gaagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcatttgttaaattattgtgcatttcactactggaacagtagtcagaaaagatagacatgcttagccaatctctatttgattga                                                         >  2:850424‑M1/1‑58 (MQ=255)                                                                                             ataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattga                                                    <  2:248376‑M1/149‑97 (MQ=255)                                                                                                 ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactcatggaactgtaatcagaaaagatagacatgcttcgccaatctgtatatgatggaattgaaaga                                                >  2:686364‑M1/1‑49 (MQ=255)                                                                                                     aaTGATATTAATAATTCCACTGCCTTTCGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcagttctctactgtaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtt                                            <  2:727154‑M1/149‑105 (MQ=255)                                                                                                           attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                      <  2:643424‑M1/149‑111 (MQ=255)                                                                                                                    tacacTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                             <  2:327731‑M1/147‑120 (MQ=255)                                                                                                                             tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                    >  2:316481‑M1/1‑21 (MQ=255)                                                                                                                             tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                           >  2:502231‑M1/1‑21 (MQ=255)                                                                                                                             tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                           <  1:502231‑M1/126‑106 (MQ=255)                                                                                                                               tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactagtggaactgtaatcagaaaagatagacatgcttagacaatccctagttgattgaattgaaagatgtttgttaaggcatggatgcgagataaag                  >  2:647499‑M1/1‑19 (MQ=255)                                                                                                                                   agagGGAAGTGCTAAataataatcgattgtttaattattgggtattgcactactggaactgtaatcagaaaaggtagcaatgcttagcgaagctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc              <  2:410848‑M1/149‑135 (MQ=255)                                                                                                                                     agGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctg            >  1:583276‑M1/1‑13 (MQ=255)                                                                                                                                          aGTGCCAAataataagcaattgtgaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg       <  2:372820‑M1/149‑142 (MQ=255)                                                                                                                                           gTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacgg      <  1:708314‑M1/149‑143 (MQ=255)                                                                                                                                            tGCTAAataataatcaattgttaaattattgtgcatttaactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggt     <  2:515228‑M1/149‑144 (MQ=255)                                                                                                                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata                   >  1:758561‑M1/1‑4 (MQ=255)                                                                                                                                              cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata                   <  2:758561‑M1/133‑130 (MQ=255)                                                                                                                                               tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcataaaagatagacatgcttagctaatctctatttgatcgaattaaatgatgtttgttaatgaatggttgcaagctaattattctgataccgtcaa  >  2:464874‑M1/1‑3 (MQ=255)                                                                                                                                               tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaa  <  1:414129‑M1/149‑147 (MQ=255)                                                                                                                                               tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                               >  1:162572‑M1/1‑3 (MQ=255)                                                                                                                                               tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                               <  2:162572‑M1/104‑102 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299354‑1299644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑