New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 4179572	44 (1.380)	5 (0.160)	5/272	3.6	12.2%	intergenic (+73/‑340)	rplA/rplJ	50S ribosomal subunit protein L1/50S ribosomal subunit protein L10
	?	NC_000913_3_pae_tpiA	= 4179682	30 (0.980)					intergenic (+183/‑230)	rplA/rplJ	50S ribosomal subunit protein L1/50S ribosomal subunit protein L10
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCT  >  NC_000913_3_pae_tpiA/4179425‑4179717                                                                                                                                                    |                                                                                                                                                  aaaGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCga                                                                                                                                                  <  1:194764/149‑1 (MQ=255)    gTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGat                                                                                                                                               >  2:44746/1‑149 (MQ=255)    gTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGACat                                                                                                                                               >  2:44723/1‑149 (MQ=255)     ttAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGata                                                                                                                                              >  1:261996/1‑149 (MQ=255)          ctctcCACCACCATGGGGGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAACCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCgg                                                                                                                                         <  1:253815/148‑1 (MQ=255)          aTCTGCACCACCATGGGTGCAGGTGTTGCAGTGGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCgg                                                                                                                                         <  2:239611/149‑1 (MQ=255)                accaccaTGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTtgtg                                                                                                                                   >  1:144087/1‑149 (MQ=255)                     caTGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAg                                                                                                                              >  2:241102/1‑149 (MQ=255)                               ggTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCga                                                                                                                                                  <  1:131793/119‑1 (MQ=255)                               ggTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCga                                                                                                                                                  >  2:131793/1‑119 (MQ=255)                                 tgtTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACtgag                                                                                                                  <  2:308961/149‑1 (MQ=255)                                  gtTTCAGGTGACTCGGGTGGCCTGAGCGCTTCTGTCAACTAATGCCTTTACGTGGGCGGTGATGTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACtgagc                                                                                                                 <  1:7481/149‑2 (MQ=255)                                  gtTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACtgagt                                                                                                                 <  2:261996/149‑1 (MQ=255)                                    tGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACtgagtga                                                                                                               <  2:77508/149‑1 (MQ=255)                                               aGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAg                                                                                                    <  2:435484/149‑1 (MQ=255)                                                          aGCGCTTCTCTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACt                                                                                         <  1:165769/149‑1 (MQ=255)                                                           gcgcTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTa                                                                                        >  2:135065/1‑149 (MQ=255)                                                                                 ttACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGAAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATgaagga                                                                  >  1:394713/1‑149 (MQ=255)                                                                                  tACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCAAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATgaaggaa                                                                 <  1:499288/149‑1 (MQ=255)                                                                                   aCGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATgaaggaag                                                                >  2:257192/1‑149 (MQ=255)                                                                                    cGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAGGGAAGc                                                               <  1:146544/149‑1 (MQ=255)                                                                                       gggCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGACACCAGGAGCATAGATAACTATGTGACTGGGGCGGAGGGAAGAAGCCaa                                                            >  1:206866/1‑149 (MQ=38)                                                                                         gCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACg                                                          <  2:144087/149‑1 (MQ=255)                                                                                                  ttGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACaa                                                 <  1:47473/149‑1 (MQ=255)                                                                                                       tACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGa                                            >  1:528528/1‑149 (MQ=255)                                                                                                            tCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACCAACTGAATAGc                                       >  2:413368/1‑149 (MQ=255)                                                                                                              ttACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTCACTATGTGACTGGTGCGAATGCAGGAACGCAACGCCGTCACATGGTGactcggga                                     >  1:545183/1‑142 (MQ=255)                                                                                                                  ccccACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTAt                                                                                       <  1:460974/95‑1 (MQ=255)                                                                                                                  ccccACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTAt                                                                                       >  2:460974/1‑95 (MQ=255)                                                                                                                    ccACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATa                               >  1:202784/1‑149 (MQ=255)                                                                                                                     cACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATgaaggaa                                                                 <  1:25389/114‑1 (MQ=255)                                                                                                                     cACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATgaaggaa                                                                 >  2:25389/1‑114 (MQ=255)                                                                                                                     cACGTATAATGCTTAATGCAGACGTATATCCGAGATATTAGGGGTGTGTCAAGGCGGCAACTGTGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAg                              >  2:263438/1‑149 (MQ=255)                                                                                                                           taATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGa                        <  2:394713/149‑1 (MQ=255)                                                                                                                              tGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGAttt                     <  2:202784/149‑1 (MQ=255)                                                                                                                                     tGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGtt              <  1:135065/149‑1 (MQ=255)                                                                                                                                      gCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg             <  1:474284/149‑1 (MQ=255)                                                                                                                                        aGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg             >  2:320999/1‑147 (MQ=255)                                                                                                                                        aGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg             >  2:320883/1‑147 (MQ=255)                                                                                                                                        aGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg             <  1:320883/147‑1 (MQ=255)                                                                                                                                        aGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg             <  1:320999/147‑1 (MQ=255)                                                                                                                                          agggtaatCCGAGATAGTGGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGc         <  2:229145/143‑1 (MQ=255)                                                                                                                                             tacacccGAGATATTCGGGTTGTGGCAACGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTg      <  1:511571/144‑1 (MQ=255)                                                                                                                                                 tCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCt  >  1:64005/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  AAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGTAAACTAATGCCTTTACGTGGGCGGTGATTTTGTCTACAATCTTACCCCCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCT  >  NC_000913_3_pae_tpiA/4179425‑4179717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.