New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 4179572	44 (1.380)	5 (0.160)	5/272	3.6	12.2%	intergenic (+73/‑340)	rplA/rplJ	50S ribosomal subunit protein L1/50S ribosomal subunit protein L10
	?	NC_000913_3_pae_tpiA	= 4179682	30 (0.980)					intergenic (+183/‑230)	rplA/rplJ	50S ribosomal subunit protein L1/50S ribosomal subunit protein L10
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCT  >  NC_000913_3_pae_tpiA/4179540‑4179823                                                                                                                                               |                                                                                                                                              ccACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATa                                                                                                                                         >  1:202784/1‑149 (MQ=255)  cACGTATAATGCTTAATGCAGACGTATATCCGAGATATTAGGGGTGTGTCAAGGCGGCAACTGTGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAg                                                                                                                                        >  2:263438/1‑149 (MQ=255)        taATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGa                                                                                                                                  <  2:394713/149‑1 (MQ=255)           tGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGAttt                                                                                                                               <  2:202784/149‑1 (MQ=255)                  tGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGtt                                                                                                                        <  1:135065/149‑1 (MQ=255)                   gCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg                                                                                                                       <  1:474284/149‑1 (MQ=255)                     aGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg                                                                                                                       >  2:320999/1‑147 (MQ=255)                     aGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg                                                                                                                       >  2:320883/1‑147 (MQ=255)                     aGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg                                                                                                                       <  1:320883/147‑1 (MQ=255)                     aGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTg                                                                                                                       <  1:320999/147‑1 (MQ=255)                       agggtaatCCGAGATAGTGGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGc                                                                                                                   <  2:229145/143‑1 (MQ=255)                          tacacccGAGATATTCGGGTTGTGGCAACGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTg                                                                                                                <  1:511571/144‑1 (MQ=255)                              tCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCt                                                                                                            >  1:64005/1‑149 (MQ=255)                                          ggggTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTcc                                                                                                >  2:99183/1‑149 (MQ=255)                                             atgtaGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGGATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTCGCCTATCCAGGCCTCCGTc                                                                                             >  1:540106/2‑149 (MQ=255)                                                  gCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGa                                                                                        >  2:454868/1‑149 (MQ=255)                                                         ggcAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTTCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAgg                                                                                 >  1:513484/1‑149 (MQ=255)                                                           cAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAg                                                                               <  1:263438/149‑1 (MQ=255)                                                            aaCTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGATGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGACCAGACTGGACCCTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGt                                                                              >  1:158354/1‑149 (MQ=255)                                                                          ccAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACtt                                                                >  2:385973/1‑149 (MQ=255)                                                                                    aGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCGAAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAACCTGGCCTATCCAGGCCTCAGTCGAAGAACGCAGGAGTTTGGCGAGACAATTTATcccatac                                                      >  2:390597/1‑145 (MQ=255)                                                                                          cTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGAc                                                <  2:64005/149‑1 (MQ=255)                                                                                               tggcTGGTGGGAATGAAGGAAGCCAACGCCGTTACAAGGTGAATAGCGACGGATAGAAAAGATTCGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGa                                           <  2:457514/146‑1 (MQ=255)                                                                                                    ggTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGTTTTGTTCGTTGGATCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCTTGCGTAGACGGTGACAGaa                                      <  2:481094/149‑1 (MQ=255)                                                                                                      tGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACGCCAGGAGTTTCGCAAGAAACTTATTGCCCTGCGTAGACGGTGACCGAACg                                    >  2:376080/1‑149 (MQ=255)                                                                                                          aaTGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGAGAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGTAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTaa                                <  2:236274/149‑1 (MQ=255)                                                                                                            tGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGa                              <  1:257192/149‑1 (MQ=255)                                                                                                                     ccaatcgcGTCACAAGCTTAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCttt                     <  2:333464/142‑1 (MQ=255)                                                                                                                     ccAACGCCGTCACAAGCTGAAGAGCGACGGATAGAAAAGATTTGTTCGTGGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCttt                     <  2:221716/149‑1 (MQ=255)                                                                                                                              tCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTg            >  1:15358/1‑149 (MQ=255)                                                                                                                                        aaTAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCt  <  2:225717/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                              CCACGTATAATGCTTAATGCAGACGTATATCCGAGATATTCGGGTTGTGGCAAGGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGGTGACAGAACGCTAAGATTATTCTTTTATATTCTGGCTTGTTTCT  >  NC_000913_3_pae_tpiA/4179540‑4179823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.