Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,756,121 Δ557 bp coding (424‑980/1413 nt) pykF → pyruvate kinase I

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1756121 1756677 557 42 [0] [0] 42 pykF pyruvate kinase I

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 17561200 (0.000)42 (1.280) 37/286 0.1 100% coding (423/1413 nt) pykF pyruvate kinase I
?NC_000913_3_pae_tpiA 1756678 = 0 (0.000)coding (981/1413 nt) pykF pyruvate kinase I

CCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1755978‑1756120
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGT  >  NC_000913_3_pae_tpiA/1756678‑1756824
                                                                                                                                                                                                                                                                                                  
CCACTGATAAATCTGTTCTCTGGAACAGCGGACTTGTGTCGGGAACGTAGGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGC                                                                                                                                               <  1:220910/149‑1
 CACTGATAAATCTGTTACCGCCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCG                                                                                                                                              >  2:182621/1‑149
  ACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGA                                                                                                                                             >  2:398358/1‑149
           TCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCCTTGAAGGTACCAAACATGGCGCCCATCTCCG                                                                                                                                    >  2:349649/1‑149
            CTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGA                                                                                                                                   <  1:433735/149‑1
                 ATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGGAGGTAACAAACATGGCGACCATCTGCGAACGTA                                                                                                                              >  1:142967/1‑149
                  TCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTAC                                                                                                                             <  1:389913/149‑1
                        ACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCAATTGAAGGTAACAAACATGGCGACCATCTTCGAACGTACCAACCG                                                                                                                       >  1:96011/1‑149
                          AGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTGTGGAAGTTACCGGCATTGAAGGTAACAAACATGGCGACCATCTGCGACTGTACCGggcggg                                                                                                                     >  2:156077/1‑143
                          AGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCG                                                                                                                     >  1:442153/1‑149
                             GAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCCACCGTACCGACCGCGTGA                                                                                                                  >  2:399898/1‑149
                              AAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGAT                                                                                                                 >  2:463707/1‑149
                                   GTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACAGGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACA                                                                                                            <  1:190114/149‑1
                                   GTTGCGGTAACGTATGAAGGTTTCACTACCGAGCTGGAAATGGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACA                                                                                                            >  1:137549/1‑149
                                      GCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCC                                                                                                         <  1:180143/149‑1
                                             CGTATCGAGGTTTCACTCCTGACCTGTCTGTGGGCAACACCGTACGGGTCGACGATGGTCTGTTCGGTATGGAAGGTACCGCCACTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCccaa                                                                                                  <  2:387952/149‑5
                                                ATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGCAATGAACAGCCGTCGAGAGTT                                                                                               >  2:478416/1‑149
                                                   AAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAA                                                                                            >  2:195846/1‑149
                                                   AAGGTTTCACTACTGACCAGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACAGGGCGACCATCTGCGAACGTCCCGACCGCGTGCTGAACAGCCGTCGCGAGTTCta                                                                                            >  2:451647/1‑147
                                                     GGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACA                                                                                          <  1:340350/149‑1
                                                       TTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAAT                                                                                        <  1:554630/149‑1
                                                         TCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGA                                                                                      <  1:195846/149‑1
                                                             TACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAAC                                                                                  >  1:66354/1‑149
                                                                TGACCTGTCTGTTGGCAACACCGTACTGGTTAAAGAAGTTCTGATCGGAATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGCCTCGAGTTCAACCATGACAACCGc                                                                               >  2:123729/1‑148
                                                                           tgGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGTCGACCATCTGCGAACGTACCGACCGCGTGATGACCAGCCGTCTCGAGCTCAACAATGACAACCGTAAACTACCCAT                                                                    >  2:364790/3‑149
                                                                           TTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAA                                                                                                                                  <  2:288160/87‑1
                                                                           TTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAA                                                                                                                                  >  1:288160/1‑87
                                                                                     CGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACGGCGCATTACCGAGGCG                                                          >  1:149542/1‑149
                                                                                               GACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTG                                                <  1:85265/149‑1
                                                                                                 CGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGT                                              <  1:398358/149‑1
                                                                                                       TCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTT                                        <  1:64473/149‑1
                                                                                                       TCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTT                                        >  2:535539/1‑149
                                                                                                         TGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGA                                      <  1:535539/149‑1
                                                                                                           ATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGGTGAAA                                    >  1:16217/1‑149
                                                                                                                  TGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGA                             >  1:300142/1‑149
                                                                                                                  TGGAAGTTACCGCCATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCATGGAGCAGATGAAACTGCTGc                             >  2:376634/1‑148
                                                                                                                                ATTGAAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTC               <  2:300142/149‑1
                                                                                                                                    AAGGTAACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACACTGACAACCGTAAACTGCGCATTACCGAAGCGGGATGCCGTGGTCACGTTGAACCTGCTGAAAAACTGGCTGCTCCGCT           >  2:333533/1‑149
                                                                                                                                         AACAAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTCTGCAGTGGTGCCGTTGAAACTGCTGAAAAACGGGATGCTCCCCTGATCG      >  2:324483/1‑149
                                                                                                                                            AAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATT                                                                   >  2:72569/1‑85
                                                                                                                                            AAACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATT                                                                   <  1:72569/85‑1
                                                                                                                                             AACATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCCCCGCTGATCGTGGT  >  1:9427/1‑149
                                                                                                                                                                                                                                                                                                  
CCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/1755978‑1756120
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGT  >  NC_000913_3_pae_tpiA/1756678‑1756824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.