Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,756,121 Δ557 bp coding (424‑980/1413 nt) pykF → pyruvate kinase I

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1756121 1756677 557 42 [0] [0] 42 pykF pyruvate kinase I

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 17561200 (0.000)42 (1.280) 37/286 0.1 100% coding (423/1413 nt) pykF pyruvate kinase I
?NC_000913_3_pae_tpiA 1756678 = 0 (0.000)coding (981/1413 nt) pykF pyruvate kinase I

CCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCT  >  NC_000913_3_pae_tpiA/1755978‑1756267
                                                                                                                                              |                                                                                                                                                   
ccacTGATAAATCTGTTCTCTGGAACAGCGGACTTGTGTCGGGAACGTAGGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggc                                                                                                                                               <  1:220910‑M1/149‑7 (MQ=255)
 cacTGATAAATCTGTTACCGCCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcg                                                                                                                                              >  2:182621‑M1/1‑142 (MQ=255)
  acTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcga                                                                                                                                             >  2:398358‑M1/1‑141 (MQ=255)
           tCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCCTTGAAGGTACCAAAcatggcgcccatctccg                                                                                                                                    >  2:349649‑M1/1‑132 (MQ=255)
            cTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcga                                                                                                                                   <  1:433735‑M1/149‑19 (MQ=255)
                 aTCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGGAGGTAACAAAcatggcgaccatctgcgaacgta                                                                                                                              >  1:142967‑M1/1‑126 (MQ=255)
                  tCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtac                                                                                                                             <  1:389913‑M1/149‑25 (MQ=255)
                        aCAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCAATTGAAGGTAACAAAcatggcgaccatcttcgaacgtaccaaccg                                                                                                                       >  1:96011‑M1/1‑119 (MQ=255)
                          aGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTGTGGAAGTTACCGGCATTGAAGGTAACAAAcatggcgaccatctgcgactgtaccgggcggg                                                                                                                     >  2:156077‑M1/1‑117 (MQ=255)
                          aGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcg                                                                                                                     >  1:442153‑M1/1‑117 (MQ=255)
                             gAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgccaccgtaccgaccgcgtga                                                                                                                  >  2:399898‑M1/1‑114 (MQ=255)
                              aaaTGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgat                                                                                                                 >  2:463707‑M1/1‑113 (MQ=255)
                                   gTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcagggcgaccatctgcgaacgtaccgaccgcgtgatgaaca                                                                                                            <  1:190114‑M1/149‑42 (MQ=255)
                                   gTTGCGGTAACGTATGAAGGTTTCACTACCGAGCTGGAAATGGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaaca                                                                                                            >  1:137549‑M1/1‑108 (MQ=255)
                                      gCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagcc                                                                                                         <  1:180143‑M1/149‑45 (MQ=255)
                                             cGTATCGAGGTTTCACTCCTGACCTGTCTGTGGGCAACACCGTACGGGTCGACGATGGTCTGTTCGGTATGGAAGGTACCGCCACTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtcccaa                                                                                                  <  2:387952‑M1/149‑52 (MQ=255)
                                                aTGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgcaatgaacagccgtcgagagtt                                                                                               >  2:478416‑M1/1‑95 (MQ=255)
                                                   aaGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaa                                                                                            >  2:195846‑M1/1‑92 (MQ=255)
                                                   aaGGTTTCACTACTGACCAGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcagggcgaccatctgcgaacgtcccgaccgcgtgctgaacagccgtcgcgagttcta                                                                                            >  2:451647‑M1/1‑92 (MQ=255)
                                                     ggTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaaca                                                                                          <  1:340350‑M1/149‑60 (MQ=255)
                                                       tttCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaat                                                                                        <  1:554630‑M1/149‑62 (MQ=255)
                                                         tCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatga                                                                                      <  1:195846‑M1/149‑64 (MQ=255)
                                                             tactGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaac                                                                                  >  1:66354‑M1/1‑82 (MQ=255)
                                                                tGACCTGTCTGTTGGCAACACCGTACTGGTTAAAGAAGTTCTGATCGGAATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgcctcgagttcaaccatgacaaccgc                                                                               >  2:123729‑M1/1‑79 (MQ=255)
                                                                           ttGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaa                                                                                                                                  <  2:288160‑M1/87‑20 (MQ=255)
                                                                           ttGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaa                                                                                                                                  >  1:288160‑M1/1‑68 (MQ=255)
                                                                           tgggcaACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatgtcgaccatctgcgaacgtaccgaccgcgtgatgaccagccgtctcgagctcaacaatgacaaccgtaaactacccat                                                                    >  2:364790‑M1/3‑68 (MQ=255)
                                                                                     cGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaacggcgcattaccgaggcg                                                          >  1:149542‑M1/1‑58 (MQ=255)
                                                                                               gACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtg                                                <  1:85265‑M1/149‑102 (MQ=255)
                                                                                                 cGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggt                                              <  1:398358‑M1/149‑104 (MQ=255)
                                                                                                       tCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgtt                                        <  1:64473‑M1/149‑110 (MQ=255)
                                                                                                       tCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgtt                                        >  2:535539‑M1/1‑40 (MQ=255)
                                                                                                         tGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttga                                      <  1:535539‑M1/149‑112 (MQ=255)
                                                                                                           aTCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccggtgaaa                                    >  1:16217‑M1/1‑36 (MQ=255)
                                                                                                                  tGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctga                             >  1:300142‑M1/1‑29 (MQ=255)
                                                                                                                  tGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccatggagcagatgaaactgctgc                             >  2:376634‑M1/1‑29 (MQ=255)
                                                                                                                                aTTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctc               <  2:300142‑M1/149‑135 (MQ=255)
                                                                                                                                    aaGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacactgacaaccgtaaactgcgcattaccgaagcgggatgccgtggtcacgttgaacctgctgaaaaactggctgctccgct           >  2:333533‑M1/1‑11 (MQ=255)
                                                                                                                                         aacaaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtctgcagtggtgccgttgaaactgctgaaaaacgggatgctcccctgatcg      >  2:324483‑M1/1‑6 (MQ=255)
                                                                                                                                            aaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcatt                                                                   >  2:72569‑M1/1‑3 (MQ=255)
                                                                                                                                            aaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcatt                                                                   <  1:72569‑M1/85‑83 (MQ=255)
                                                                                                                                             aacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgccccgctgatcgtggt  >  1:9427‑M1/1‑2 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCT  >  NC_000913_3_pae_tpiA/1755978‑1756267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.