New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 404245639 (1.180)5 (0.160) 4/268 4.2 14.0% coding (42/987 nt) srkA Cpx stress response Thr/Ser protein kinase; MazF antagonist protein
?NC_000913_3_pae_tpiA = 4042486 25 (0.810)coding (72/987 nt) srkA Cpx stress response Thr/Ser protein kinase; MazF antagonist protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4042302‑4042456
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cacccggatCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTGGAAAGTAAAAGCGCTGTTATTCATCCCATCATCCGGAAAACATTACGAATGACGAAGGATATCATTTCACGATGATTTTGGTGGCGCCGCTTACAA  <  NC_000913_3_pae_tpiA/4042486‑4042343
                                                                                                                                                                                                                                                                                                           
GGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAC                                                                                                                                                        >  2:313987/1‑149
                                               GCGGCGCCACCAAAAGCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTA                                                                                                         <  2:332107/149‑1
                                                     CCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGG                                                                                                              >  1:328568/1‑138
                                                     CCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGG                                                                                                              <  2:328568/138‑1
                                                                                                             TGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGG                                                                                                              >  1:368076/1‑82
                                                                                                             TGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGG                                                                                                              <  2:368076/82‑1
                                                                                                                                                  CACCCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTGGAAAGTAAAAGCGCTGTTATTCATCCCATCATCCGGAAAACATTACGAATGACGAAGGATATCATTTCACGATGATTTTGGTGGCGCCGCTT      >  2:301942/1‑149
                                                                                                                                                     CCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTGGAAAGTAAAAGCGCTGTTATTCATC                                                                         >  1:43921/1‑79
                                                                                                                                                     CCGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTGGAAAGTAAAAGCGCTGTTATTCATC                                                                         <  2:43921/79‑1
                                                                                                                                                      CGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTGGAAAGTAAAAGCGCTGTTATTCATCCCATCATCCGGAAAACATTACGAATGACGAAGGATATCATTTCACGATGGTTTTGGTGGCGCCGCTTACAA  >  1:15173/1‑149
                                                                                                                                                      CGGATCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTGGAAAGTAAAAGCGCTGTTATTCATCCCATCATCCGGAAAACATTACGAATGACGAAGGATATCATTTCACGATGATTTTGGTGGCGCCGCTTACAA  <  1:326830/149‑1
                                                                                                                                                                                                                                                                                                           
GGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4042302‑4042456
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cacccggatCCCATGCTCAAACAGAGCGTCCATGATGGTATCCGGGTGTAGTGTCTGGAAAGTAAAAGCGCTGTTATTCATCCCATCATCCGGAAAACATTACGAATGACGAAGGATATCATTTCACGATGATTTTGGTGGCGCCGCTTACAA  <  NC_000913_3_pae_tpiA/4042486‑4042343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.