New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 404245639 (1.180)5 (0.160) 4/268 4.2 14.0% coding (42/987 nt) srkA Cpx stress response Thr/Ser protein kinase; MazF antagonist protein
?NC_000913_3_pae_tpiA = 4042486 25 (0.810)coding (72/987 nt) srkA Cpx stress response Thr/Ser protein kinase; MazF antagonist protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTG  >  NC_000913_3_pae_tpiA/4042317‑4042599
                                                                                                                                           |                                                                                                                                               
tCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATg                                                                                                                                        >  2:477818/1‑149 (MQ=255)
   cggcgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACTAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGAc                                                                                                                                     <  1:192087/149‑1 (MQ=255)
   cagcgTAATTAAAGAGTAAACGCTTGTAAGCGCCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGAc                                                                                                                                     <  1:47189/147‑1 (MQ=255)
      cgTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGCACGct                                                                                                                                  >  2:218695/1‑149 (MQ=255)
        tAATTAAAGAGTAAAAGCTTGTAAGCGGCGGCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGctct                                                                                                                                <  2:303909/149‑1 (MQ=255)
         aaTTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGCCACTCCACCCGGATACCATCATGGACGctcgg                                                                                                                               >  1:214476/1‑147 (MQ=255)
          aTTAAAGAGTAAAAGCTTGTAAGCGGATCCACCAAAGTCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGACGACGGGATGAATAACAGCGCTGTTACTTTCCAGACACTACCCCCGGATACCATCATGGACGCTCTGt                                                                                                                              <  1:25168/149‑1 (MQ=255)
              aaGAGTAAAAGGTTGGAAGCCGCGCCACCAAAATCATCCTGAAATGGTAGCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACTGCGCTTTTACTTTCCAGACACTACACTCGGATACCATCATGGACGCTCTGTTTGa                                                                                                                          <  2:36267/149‑1 (MQ=255)
               agagTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAg                                                                                                                         <  2:180017/149‑1 (MQ=255)
                   tAAAAGCTTGTAAGCGGCGCCACCAAAATCATCATGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACCACACCCGGATACCATCATGGACGCTCTGTTTGAGCATg                                                                                                                     <  1:481318/149‑1 (MQ=255)
                   tAAAAGCGTGTAAGCGGCGCCACGAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCTGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATg                                                                                                                     <  2:561472/149‑1 (MQ=255)
                          ttGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCg                                                                                                              >  1:326830/1‑149 (MQ=255)
                          ttGTAAGCGGCGCCACCAAAACCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCg                                                                                                              <  1:15173/149‑1 (MQ=255)
                              aaGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTg                                                                                                          <  2:301942/149‑1 (MQ=255)
                                   gcgcCACCAAAATCATCGTGAAATGATATCCCTCGTCATGCGTAATGATTTCCGGATGATGGGATGAATAACCGCGCTTTTACTTTCCAGACACTCAACCCGGATACCATCATGGATCTCCTGTCTGCGCATGGGCCCCCGCTGGATTc                                                                                                     >  2:235997/1‑149 (MQ=255)
                                                      gAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAAc                                                                                  <  2:326830/149‑1 (MQ=255)
                                                            atatCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACCCCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTAt                                                                            <  1:444303/149‑1 (MQ=255)
                                                               tCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGATTTTACTTTCCAGACACTACACCCGGGTACCATCATGGACGCTCTGTTTGAGCATGGGATCAGAGTGGATTCCAGTCTTACCCCGCTTAACCGATATgca                                                                         >  2:251810/1‑147 (MQ=255)
                                                                   tCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAAcc                                                                     <  2:192731/149‑1 (MQ=255)
                                                                    ccgaatccGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGAGGCTGTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCg                                                                    <  1:161170/142‑1 (MQ=255)
                                                                          tCGTAATGTTTTCCGGATGATGGGATGAATAAGAGCGCTTTTACTTTCCAGTCACTCCACTCGGATATCATCATGGACGCTCTGTTTGTGGATGGGATCCGGGGGGATTCCGGTCTTACCCCGCTTAACAGCTATGAACACCGTGTCTa                                                              <  2:207289/149‑1 (MQ=255)
                                                                           cGTAATGTTTTCCGGAGGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGGTCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTAt                                                             <  2:437369/149‑1 (MQ=255)
                                                                             tAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTATTACCCCGCTTAACGGCTATGCAAACAGTGTCTATCa                                                           >  1:562425/1‑149 (MQ=255)
                                                                                    ttCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAg                                                    <  1:313987/149‑1 (MQ=255)
                                                                                                 gATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCgg                                                                                                             <  1:43921/79‑1 (MQ=255)
                                                                                                 gATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCgg                                                                                                             >  2:43921/1‑79 (MQ=255)
                                                                                                        aaCAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGc                                                                               >  2:394938/1‑102 (MQ=255)
                                                                                                        aaCAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGc                                                                               <  1:394938/102‑1 (MQ=255)
                                                                                                            gcgcTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACCGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTgtc                            <  1:376261/149‑1 (MQ=255)
                                                                                                            gcgcTTGTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTgtc                            <  1:89960/149‑1 (MQ=255)
                                                                                                             cgcTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTgtcg                           <  2:154045/149‑1 (MQ=255)
                                                                                                              gcTTTTACTTTCCAGACACTACACCCGGGTACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTgtcgt                          <  2:138201/149‑1 (MQ=255)
                                                                                                                           aaacagTAAACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATACGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGc             <  2:214476/147‑1 (MQ=255)
                                                                                                                           aGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGc             >  2:68574/1‑149 (MQ=255)
                                                                                                                            gACACTACACCCGGATACCATGATGGTCGCTCTGTTTGAGCATGCGGTTCGTGTGGATTCCGGTCTTACCCCGCGTAACAGCTATTAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGcc            <  2:399890/149‑1 (MQ=255)
                                                                                                                               acTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTg         >  2:135919/1‑149 (MQ=255)
                                                                                                                                 tACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGaa       >  1:525981/1‑149 (MQ=255)
                                                                                                                                      cgggATACCAGCATGGACGCTCTGGTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTg  <  2:551344/147‑1 (MQ=255)
                                                                                                                                      ccGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTg  <  1:68574/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
TCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTG  >  NC_000913_3_pae_tpiA/4042317‑4042599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.