New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 4042456	39 (1.180)	5 (0.160)	4/268	4.2	14.0%	coding (42/987 nt)	srkA	Cpx stress response Thr/Ser protein kinase; MazF antagonist protein
	?	NC_000913_3_pae_tpiA	= 4042486	25 (0.810)					coding (72/987 nt)	srkA	Cpx stress response Thr/Ser protein kinase; MazF antagonist protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAA  >  NC_000913_3_pae_tpiA/4042343‑4042613                                                                                                                                                |                                                                                                                                ttGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCg                                                                                                                            >  1:326830/1‑149 (MQ=255) ttGTAAGCGGCGCCACCAAAACCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCg                                                                                                                            <  1:15173/149‑1 (MQ=255)     aaGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTg                                                                                                                        <  2:301942/149‑1 (MQ=255)          gcgcCACCAAAATCATCGTGAAATGATATCCCTCGTCATGCGTAATGATTTCCGGATGATGGGATGAATAACCGCGCTTTTACTTTCCAGACACTCAACCCGGATACCATCATGGATCTCCTGTCTGCGCATGGGCCCCCGCTGGATTc                                                                                                                   >  2:235997/1‑149 (MQ=255)                             gAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAAc                                                                                                <  2:326830/149‑1 (MQ=255)                                   atatCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACCCCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTAt                                                                                          <  1:444303/149‑1 (MQ=255)                                      tCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGATTTTACTTTCCAGACACTACACCCGGGTACCATCATGGACGCTCTGTTTGAGCATGGGATCAGAGTGGATTCCAGTCTTACCCCGCTTAACCGATATgca                                                                                       >  2:251810/1‑147 (MQ=255)                                          tCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAAcc                                                                                   <  2:192731/149‑1 (MQ=255)                                           ccgaatccGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGAGGCTGTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCg                                                                                  <  1:161170/142‑1 (MQ=255)                                                 tCGTAATGTTTTCCGGATGATGGGATGAATAAGAGCGCTTTTACTTTCCAGTCACTCCACTCGGATATCATCATGGACGCTCTGTTTGTGGATGGGATCCGGGGGGATTCCGGTCTTACCCCGCTTAACAGCTATGAACACCGTGTCTa                                                                            <  2:207289/149‑1 (MQ=255)                                                  cGTAATGTTTTCCGGAGGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGGTCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTAt                                                                           <  2:437369/149‑1 (MQ=255)                                                    tAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTATTACCCCGCTTAACGGCTATGCAAACAGTGTCTATCa                                                                         >  1:562425/1‑149 (MQ=255)                                                           ttCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAg                                                                  <  1:313987/149‑1 (MQ=255)                                                                        gATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCgg                                                                                                                           >  2:43921/1‑79 (MQ=255)                                                                        gATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCgg                                                                                                                           <  1:43921/79‑1 (MQ=255)                                                                               aaCAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGc                                                                                             >  2:394938/1‑102 (MQ=255)                                                                               aaCAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGc                                                                                             <  1:394938/102‑1 (MQ=255)                                                                                   gcgcTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACCGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTgtc                                          <  1:376261/149‑1 (MQ=255)                                                                                   gcgcTTGTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTgtc                                          <  1:89960/149‑1 (MQ=255)                                                                                    cgcTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTgtcg                                         <  2:154045/149‑1 (MQ=255)                                                                                     gcTTTTACTTTCCAGACACTACACCCGGGTACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTgtcgt                                        <  2:138201/149‑1 (MQ=255)                                                                                                  aaacagTAAACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATACGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGc                           <  2:214476/147‑1 (MQ=255)                                                                                                  aGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGc                           >  2:68574/1‑149 (MQ=255)                                                                                                   gACACTACACCCGGATACCATGATGGTCGCTCTGTTTGAGCATGCGGTTCGTGTGGATTCCGGTCTTACCCCGCGTAACAGCTATTAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGcc                          <  2:399890/149‑1 (MQ=255)                                                                                                      acTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTg                       >  2:135919/1‑149 (MQ=255)                                                                                                        tACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGaa                     >  1:525981/1‑149 (MQ=255)                                                                                                             cgggATACCAGCATGGACGCTCTGGTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTg                <  2:551344/147‑1 (MQ=255)                                                                                                             ccGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTg                <  1:68574/149‑1 (MQ=255)                                                                                                                        catGGACGCTCTGTTGGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATc     <  2:444514/149‑1 (MQ=255)                                                                                                                           ggACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCaaa  >  1:493431/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                TTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATACCATCATGGACGCTCTGTTTGAGCATGGGATCCGGGTGGATTCCGGTCTTACCCCGCTTAACAGCTATGAAAACCGTGTCTATCAATTTCAGGACGAAGATCGTCGACGTTTTGTCGTCAAATTTTATCGCCCTGAACGTTGGACAGCCGATCAAA  >  NC_000913_3_pae_tpiA/4042343‑4042613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.