Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,756,121 Δ557 bp coding (424‑980/1413 nt) pykF → pyruvate kinase I

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1756121 1756677 557 98 [0] [0] 98 pykF pyruvate kinase I

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 17561200 (0.000)98 (0.830) 80/280 0.4 100% coding (423/1413 nt) pykF pyruvate kinase I
?NC_000913_3_pae_tpiA 1756678 = 0 (0.000)coding (981/1413 nt) pykF pyruvate kinase I

CCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGT  >  NC_000913_3_pae_tpiA/1756530‑1756824
                                                                                                                                                    |                                                                                                                                                  
tttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaac                                                                                                                                                    >  1:123600‑M2/149‑149 (MQ=255)
  tcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCAt                                                                                                                                                  <  2:1089067‑M2/3‑1 (MQ=255)
    accactgataaatctgttatcggcaacagcgaaatggttgcggtaacggatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATgg                                                                                                                                                >  1:106757‑M2/145‑149 (MQ=255)
     ccactgataaatctgttatcggccaccgctaaatggttgcggttccccgccaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGc                                                                                                                                               <  1:1008573‑M2/6‑1 (MQ=255)
        ctgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGAc                                                                                                                                            <  1:1953159‑M2/9‑1 (MQ=255)
          gataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCa                                                                                                                                          >  1:402534‑M2/139‑149 (MQ=255)
           ataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCAt                                                                                                                                         <  2:868623‑M2/12‑1 (MQ=255)
                    ttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACg                                                                                                                                >  2:1247270‑M2/129‑149 (MQ=255)
                      atcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtaatggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTa                                                                                                                              >  2:760412‑M2/127‑149 (MQ=255)
                             acagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccggactgattgacgatggtctgatcggtatggaggttaccgccattgagtgtaacaaaGATGGCTACCATCTGCGAAGGTaccggccg                                                                                                                       >  1:181000‑M2/120‑149 (MQ=255)
                              cagcgaaatggttgcggtaacgtatgatggtttcactattgacctgtctgttggcaacaccgtactggttgacgatggtctgctcggtatggaagttggattgattgaaggtaacaaaCATGGTGACTATCTGCGAACGTACCGACcgc                                                                                                                      <  2:347893‑M2/31‑1 (MQ=255)
                               agcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACcgcg                                                                                                                     >  1:194218‑M2/118‑149 (MQ=255)
                                gcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGt                                                                                                                    <  1:786126‑M2/33‑1 (MQ=255)
                                gcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCAACCATCAACCAACGTACAGACCACGt                                                                                                                    >  1:407657‑M2/117‑149 (MQ=255)
                                   aaatggttggggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGtgat                                                                                                                 <  1:3813‑M2/36‑1 (MQ=255)
                                   aaacggtcgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgagggtctgatcggtctggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGtgat                                                                                                                 <  1:466904‑M2/36‑1 (MQ=255)
                                          tgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACAGCGTGATGACCAGc                                                                                                          >  2:577444‑M2/107‑149 (MQ=255)
                                                aaagtatgacggtttcattactaacctgtctgttggcaacaccggattggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCCAACGTACCGACCGCGTGATGAACAGCCGtctc                                                                                                    <  2:481671‑M2/49‑1 (MQ=255)
                                                  cgtatgaaggtttcactactgacctgtctggtggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCAGGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGa                                                                                                  <  2:346042‑M2/51‑1 (MQ=255)
                                                     atgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGtt                                                                                               <  1:1076919‑M2/54‑1 (MQ=255)
                                                       gaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTCAGAACGTACTGACCGGGTGCTGAACAGCCGtctcgatgtga                                                                                             >  2:1449494‑M2/94‑144 (MQ=255)
                                                         aggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaac                                                                                           >  1:1331914‑M2/92‑149 (MQ=255)
                                                           gtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGc                                                                                                                                               >  2:564898‑M2/90‑95 (MQ=255)
                                                           gtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGc                                                                                                                                               <  1:564898‑M2/6‑1 (MQ=255)
                                                           gtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGGGTGATGAACAGCGGTCTCGGGTTccacaa                                                                                         >  2:425815‑M2/90‑149 (MQ=255)
                                                           gtttcactactgacctgtctgttggcaacaccgtactggttgacgatgatctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGttcaacca                                                                                         >  2:1874542‑M2/90‑147 (MQ=255)
                                                           gtttcactactgacctgtctgttggcaacaccgtactggtcgacgatggtctgatcgggttggcagttacccccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATAAACAGCCGTCTCGAGTTcaacaa                                                                                         <  2:1920724‑M2/60‑1 (MQ=255)
                                                           gtttcactactgacctgtctcttggcaacaccatactggttgacgatgggctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGAGAACGTAACGACCGCGTGATGAACAGCCGTGTCGATATcaacaa                                                                                         >  2:580120‑M2/90‑149 (MQ=255)
                                                                 ctactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTa                                                                                                                              >  2:342854‑M2/84‑106 (MQ=255)
                                                                 ctactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTa                                                                                                                              <  1:342854‑M2/23‑1 (MQ=255)
                                                                 ctactgacctgtctgttggcaacaccgtacgggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGTACAGCCGTCTCGAGTTCAACAATGACaa                                                                                   <  1:1670248‑M2/66‑1 (MQ=255)
                                                                  cactgacctgtctgttggcaacaccgtactggttgaagatggtctggtcggtatggaatttaccgccattgaaggtaacaaaCAGGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAAc                                                                                  <  2:1999020‑M2/67‑1 (MQ=255)
                                                                   actgatctggctgttggcaacaccggactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAAcc                                                                                 <  2:2033619‑M2/68‑1 (MQ=255)
                                                                   actgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCTAGTTCAACAATGACAAcc                                                                                 <  2:247877‑M2/68‑1 (MQ=255)
                                                                      gacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTa                                                                              >  2:1342743‑M2/79‑149 (MQ=255)
                                                                        cctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTaaa                                                                            <  1:891556‑M2/73‑1 (MQ=255)
                                                                        cctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTaaa                                                                            >  1:2021953‑M2/77‑149 (MQ=255)
                                                                         ctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACACTGACAACCgtaagc                                                                           >  2:1729624‑M2/76‑147 (MQ=255)
                                                                         ctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAAc                                                                           >  1:1579199‑M2/76‑149 (MQ=255)
                                                                          tgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACt                                                                          <  1:1536988‑M2/75‑1 (MQ=255)
                                                                             ctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTATCGACCCCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGtaaaccacg                                                                       >  2:1010055‑M2/72‑145 (MQ=255)
                                                                             ctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTgcg                                                                       >  1:1576807‑M2/72‑149 (MQ=255)
                                                                             ctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTgcg                                                                       <  2:1617227‑M2/78‑1 (MQ=255)
                                                                                 tggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCAtt                                                                   <  1:349494‑M2/82‑1 (MQ=255)
                                                                                   gcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGGCCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAc                                                                 >  1:1970539‑M2/66‑149 (MQ=255)
                                                                                   gcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAc                                                                 <  1:1414057‑M2/84‑1 (MQ=255)
                                                                                     aacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCg                                                               >  2:1070303‑M2/64‑149 (MQ=255)
                                                                                     aacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGCTCAACAATCACAACCGTAAACTCCGCATTaccc                                                               >  2:834490‑M2/64‑148 (MQ=255)
                                                                                      acaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGa                                                              >  2:1426508‑M2/63‑149 (MQ=255)
                                                                                       caccgtactggttgacgatgggctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGaa                                                             <  2:1596433‑M2/88‑1 (MQ=255)
                                                                                        accgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAg                                                            >  2:511496‑M2/61‑149 (MQ=255)
                                                                                        accgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAg                                                            >  1:1179642‑M2/61‑149 (MQ=255)
                                                                                        accgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAg                                                            >  1:1064712‑M2/61‑149 (MQ=255)
                                                                                          cgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCg                                                          >  2:108660‑M2/59‑149 (MQ=255)
                                                                                            tactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGt                                                        >  1:671471‑M2/57‑149 (MQ=255)
                                                                                             actggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAAAGATAATATCCTTAAACTGCGCATTACCGAAGCGGTa                                                       <  1:1553100‑M2/94‑1 (MQ=255)
                                                                                                 gttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGcc                                                   <  2:1064712‑M2/98‑1 (MQ=255)
                                                                                                    gacgatggtctgatcgggatggaagttaccgccattgaatgtagcaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtg                                                <  2:1841102‑M2/101‑1 (MQ=255)
                                                                                                          ggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCg                                          >  1:1647112‑M2/43‑149 (MQ=255)
                                                                                                           gtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGt                                         >  1:676900‑M2/42‑149 (MQ=255)
                                                                                                            tctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGtt                                        <  1:12503‑M2/109‑1 (MQ=255)
                                                                                                              tgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACATTGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGa                                      >  1:1781376‑M2/39‑149 (MQ=255)
                                                                                                              tgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGa                                      <  1:1247270‑M2/111‑1 (MQ=255)
                                                                                                                 tcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCTCATTACCGTAGCGGTATGCCGTGGTGCCGTTGAAAc                                   >  2:796135‑M2/36‑149 (MQ=255)
                                                                                                                   ggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATTTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActg                                 <  2:209066‑M2/116‑1 (MQ=255)
                                                                                                                    gtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgc                                >  1:1566024‑M2/33‑149 (MQ=255)
                                                                                                                     tagggaagttaccgccattgaaggcaacaaaCCTGGCGACCATCTGCGAACGTACCGACCGCATGATGACCAGCCGTCACGAGTTCAACAATGCCAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGCCGTGCCGTTAAACctgct                               >  2:865266‑M2/32‑149 (MQ=255)
                                                                                                                       tggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGACGCGGTATGCCATGGTGCCGTTGAAACTGCTGa                             >  2:1199480‑M2/30‑149 (MQ=255)
                                                                                                                         gaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaaa                           >  1:900334‑M2/28‑149 (MQ=255)
                                                                                                                             ttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                       >  2:161539‑M2/24‑149 (MQ=255)
                                                                                                                             ttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAACGGTATGCCGTGGTTCCCTTGAACatgctgacagatt                       >  2:2035932‑M2/24‑143 (MQ=255)
                                                                                                                              taccgccatggaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTg                      <  1:637461‑M2/127‑1 (MQ=255)
                                                                                                                              gagtaccagtgaaggtaacaaaCATGGCGACCATCTGACAACGTAGCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCACATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTg                      <  2:2076838‑M2/127‑1 (MQ=255)
                                                                                                                               accgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTgg                     <  1:108660‑M2/128‑1 (MQ=255)
                                                                                                                                ccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGa                    <  1:760412‑M2/129‑1 (MQ=255)
                                                                                                                                ccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGa                    >  2:1183069‑M2/21‑149 (MQ=255)
                                                                                                                                ccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGa                    <  2:2057097‑M2/129‑1 (MQ=255)
                                                                                                                                 cgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAACCTGCTGAAACACTGGAt                   >  1:880678‑M2/20‑149 (MQ=255)
                                                                                                                                 cgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGGTGAAACTGCTGAAAAActgggt                   >  1:668840‑M2/20‑147 (MQ=255)
                                                                                                                                 cgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGGGGTGCCGTTGAAACTGCTGAAAAACTGGAt                   >  1:954667‑M2/20‑149 (MQ=255)
                                                                                                                                  gccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATg                  >  1:1460661‑M2/19‑149 (MQ=255)
                                                                                                                                    cactgaaggtaacaaaCATGGCGACCATCTGCGAACATCCCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCt                <  1:857851‑M2/133‑1 (MQ=255)
                                                                                                                                     attgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTc               >  1:1673765‑M2/16‑149 (MQ=255)
                                                                                                                                      ttgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGCAACTGCTGAAAAACTGGATGCTcc              >  2:1088475‑M2/15‑149 (MQ=255)
                                                                                                                                         aaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCg                                                          >  2:1348893‑M2/12‑102 (MQ=255)
                                                                                                                                         aaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCg                                                          <  1:1348893‑M2/91‑1 (MQ=255)
                                                                                                                                            gtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGc                                                                                                          >  1:361412‑M2/9‑51 (MQ=255)
                                                                                                                                            gtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGc                                                                                                          <  2:361412‑M2/43‑1 (MQ=255)
                                                                                                                                             taacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAAAAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGCAAAACTGGATGCTCCGCTGATc       >  1:257018‑M2/8‑149 (MQ=255)
                                                                                                                                              aacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCg      >  1:290721‑M2/7‑149 (MQ=255)
                                                                                                                                               acaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGa                                                                                                  >  1:1627501‑M2/6‑56 (MQ=255)
                                                                                                                                               acaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGa                                                                                                  <  2:1627501‑M2/51‑1 (MQ=255)
                                                                                                                                                caaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTg    >  2:125992‑M2/5‑149 (MQ=255)
                                                                                                                                                caaaCAGGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTg    <  2:181000‑M2/145‑1 (MQ=255)
                                                                                                                                                 aaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTgg   >  1:1204084‑M2/4‑149 (MQ=255)
                                                                                                                                                  aaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGt  >  1:1430858‑M2/3‑149 (MQ=255)
                                                                                                                                                   aCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCgg                                                         >  2:735612‑M2/2‑93 (MQ=255)
                                                                                                                                                   aCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCgg                                                         <  1:735612‑M2/92‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGT  >  NC_000913_3_pae_tpiA/1756530‑1756824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.