Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,756,121	Δ557 bp	coding (424‑980/1413 nt)	pykF →	pyruvate kinase I

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1756121	1756677	557	98 [0]	[0] 98	pykF	pyruvate kinase I

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1756120	0 (0.000)	98 (0.830)	80/280	0.4	100%	coding (423/1413 nt)	pykF	pyruvate kinase I
	?	NC_000913_3_pae_tpiA	1756678 =	0 (0.000)					coding (981/1413 nt)	pykF	pyruvate kinase I

TTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCT  >  NC_000913_3_pae_tpiA/1755973‑1756267                                                                                                                                                    |                                                                                                                                                    tttCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAc                                                                                                                                                    >  1:123600‑M1/1‑148 (MQ=255)   tCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcat                                                                                                                                                  <  2:1089067‑M1/149‑4 (MQ=255)     accacTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGGATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatgg                                                                                                                                                >  1:106757‑M1/1‑144 (MQ=255)      ccacTGATAAATCTGTTATCGGCCACCGCTAAATGGTTGCGGTTCCCCGCCAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggc                                                                                                                                               <  1:1008573‑M1/149‑7 (MQ=255)         cTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgac                                                                                                                                            <  1:1953159‑M1/149‑10 (MQ=255)           gATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgacca                                                                                                                                          >  1:402534‑M1/1‑138 (MQ=255)            aTAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccat                                                                                                                                         <  2:868623‑M1/149‑13 (MQ=255)                     ttATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacg                                                                                                                                >  2:1247270‑M1/1‑128 (MQ=255)                       aTCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTAATGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgta                                                                                                                              >  2:760412‑M1/1‑126 (MQ=255)                              aCAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGGACTGATTGACGATGGTCTGATCGGTATGGAGGTTACCGCCATTGAGTGTAACAAAgatggctaccatctgcgaaggtaccggccg                                                                                                                       >  1:181000‑M1/1‑119 (MQ=255)                               cAGCGAAATGGTTGCGGTAACGTATGATGGTTTCACTATTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGCTCGGTATGGAAGTTGGATTGATTGAAGGTAACAAAcatggtgactatctgcgaacgtaccgaccgc                                                                                                                      <  2:347893‑M1/149‑32 (MQ=255)                                aGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcg                                                                                                                     >  1:194218‑M1/1‑117 (MQ=255)                                 gCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgt                                                                                                                    <  1:786126‑M1/149‑34 (MQ=255)                                 gCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcaaccatcaaccaacgtacagaccacgt                                                                                                                    >  1:407657‑M1/1‑116 (MQ=255)                                    aaaTGGTTGGGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgat                                                                                                                 <  1:3813‑M1/149‑37 (MQ=255)                                    aaaCGGTCGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGAGGGTCTGATCGGTCTGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgat                                                                                                                 <  1:466904‑M1/149‑37 (MQ=255)                                           tGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgacagcgtgatgaccagc                                                                                                          >  2:577444‑M1/1‑106 (MQ=255)                                                 aaagtatGACGGTTTCATTACTAACCTGTCTGTTGGCAACACCGGATTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgccaacgtaccgaccgcgtgatgaacagccgtctc                                                                                                    <  2:481671‑M1/146‑50 (MQ=255)                                                   cGTATGAAGGTTTCACTACTGACCTGTCTGGTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcagggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcga                                                                                                  <  2:346042‑M1/149‑52 (MQ=255)                                                      aTGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagtt                                                                                               <  1:1076919‑M1/149‑55 (MQ=255)                                                        gAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctcagaacgtactgaccgggtgctgaacagccgtctcgatgtga                                                                                             >  2:1449494‑M1/1‑93 (MQ=255)                                                          aGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaac                                                                                           >  1:1331914‑M1/1‑91 (MQ=255)                                                            gTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgggtgatgaacagcggtctcgggttccacaa                                                                                         >  2:425815‑M1/1‑89 (MQ=255)                                                            gTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggc                                                                                                                                               >  2:564898‑M1/1‑89 (MQ=255)                                                            gTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggc                                                                                                                                               <  1:564898‑M1/95‑7 (MQ=255)                                                            gTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGATCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacca                                                                                         >  2:1874542‑M1/1‑89 (MQ=255)                                                            gTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTCGACGATGGTCTGATCGGGTTGGCAGTTACCCCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgataaacagccgtctcgagttcaacaa                                                                                         <  2:1920724‑M1/149‑61 (MQ=255)                                                            gTTTCACTACTGACCTGTCTCTTGGCAACACCATACTGGTTGACGATGGGCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgagaacgtaacgaccgcgtgatgaacagccgtgtcgatatcaacaa                                                                                         >  2:580120‑M1/1‑89 (MQ=255)                                                                  ctactGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgta                                                                                                                              >  2:342854‑M1/1‑83 (MQ=255)                                                                  ctactGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgta                                                                                                                              <  1:342854‑M1/106‑24 (MQ=255)                                                                  ctactGACCTGTCTGTTGGCAACACCGTACGGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgtacagccgtctcgagttcaacaatgacaa                                                                                   <  1:1670248‑M1/149‑67 (MQ=255)                                                                   cactgACCTGTCTGTTGGCAACACCGTACTGGTTGAAGATGGTCTGGTCGGTATGGAATTTACCGCCATTGAAGGTAACAAAcagggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaac                                                                                  <  2:1999020‑M1/148‑68 (MQ=255)                                                                    actGATCTGGCTGTTGGCAACACCGGACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaacc                                                                                 <  2:2033619‑M1/149‑69 (MQ=255)                                                                    actGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctctagttcaacaatgacaacc                                                                                 <  2:247877‑M1/149‑69 (MQ=255)                                                                       gACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgta                                                                              >  2:1342743‑M1/1‑78 (MQ=255)                                                                         ccTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaa                                                                            <  1:891556‑M1/149‑74 (MQ=255)                                                                         ccTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaa                                                                            >  1:2021953‑M1/1‑76 (MQ=255)                                                                          ctgtctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacactgacaaccgtaagc                                                                           >  2:1729624‑M1/1‑75 (MQ=255)                                                                          ctgtctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaac                                                                           >  1:1579199‑M1/1‑75 (MQ=255)                                                                           tgtctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaact                                                                          <  1:1536988‑M1/149‑76 (MQ=255)                                                                              ctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtatcgaccccgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaaccacg                                                                       >  2:1010055‑M1/1‑71 (MQ=255)                                                                              ctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcg                                                                       >  1:1576807‑M1/1‑71 (MQ=255)                                                                              ctgtTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcg                                                                       <  2:1617227‑M1/149‑79 (MQ=255)                                                                                  tGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcatt                                                                   <  1:349494‑M1/149‑83 (MQ=255)                                                                                    gCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccggccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattac                                                                 >  1:1970539‑M1/1‑65 (MQ=255)                                                                                    gCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattac                                                                 <  1:1414057‑M1/149‑85 (MQ=255)                                                                                      aaCACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccg                                                               >  2:1070303‑M1/1‑63 (MQ=255)                                                                                      aaCACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagctcaacaatcacaaccgtaaactccgcattaccc                                                               >  2:834490‑M1/1‑63 (MQ=255)                                                                                       acacCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccga                                                              >  2:1426508‑M1/1‑62 (MQ=255)                                                                                        cacCGTACTGGTTGACGATGGGCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaa                                                             <  2:1596433‑M1/149‑89 (MQ=255)                                                                                         acCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaag                                                            >  2:511496‑M1/1‑60 (MQ=255)                                                                                         acCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaag                                                            >  1:1179642‑M1/1‑60 (MQ=255)                                                                                         acCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaag                                                            >  1:1064712‑M1/1‑60 (MQ=255)                                                                                           cGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcg                                                          >  2:108660‑M1/1‑58 (MQ=255)                                                                                             tACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggt                                                        >  1:671471‑M1/1‑56 (MQ=255)                                                                                              aCTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaaagataatatccttaaactgcgcattaccgaagcggta                                                       <  1:1553100‑M1/149‑95 (MQ=255)                                                                                                  gTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgcc                                                   <  2:1064712‑M1/149‑99 (MQ=255)                                                                                                     gACGATGGTCTGATCGGGATGGAAGTTACCGCCATTGAATGTAGCAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtg                                                <  2:1841102‑M1/149‑102 (MQ=255)                                                                                                           ggTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccg                                          >  1:1647112‑M1/1‑42 (MQ=255)                                                                                                            gTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgt                                         >  1:676900‑M1/1‑41 (MQ=255)                                                                                                             tCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgtt                                        <  1:12503‑M1/149‑110 (MQ=255)                                                                                                               tGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacattgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttga                                      >  1:1781376‑M1/1‑38 (MQ=255)                                                                                                               tGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttga                                      <  1:1247270‑M1/149‑112 (MQ=255)                                                                                                                  tCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgctcattaccgtagcggtatgccgtggtgccgttgaaac                                   >  2:796135‑M1/1‑35 (MQ=255)                                                                                                                    ggTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatttgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactg                                 <  2:209066‑M1/149‑117 (MQ=255)                                                                                                                     gTATGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgc                                >  1:1566024‑M1/1‑32 (MQ=255)                                                                                                                      tagggaagTTACCGCCATTGAAGGCAACAAAcctggcgaccatctgcgaacgtaccgaccgcatgatgaccagccgtcacgagttcaacaatgccaaccgtaaactgcgcattaccgaagcggtatgccgccgtgccgttaaacctgct                               >  2:865266‑M1/4‑31 (MQ=255)                                                                                                                        tGGAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgacgcggtatgccatggtgccgttgaaactgctga                             >  2:1199480‑M1/1‑29 (MQ=255)                                                                                                                          gAAGTTACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaa                           >  1:900334‑M1/1‑27 (MQ=255)                                                                                                                              ttACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaact                       >  2:161539‑M1/1‑23 (MQ=255)                                                                                                                              ttACCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaaacggtatgccgtggttcccttgaacatgctgacagatt                       >  2:2035932‑M1/1‑23 (MQ=255)                                                                                                                               tACCGCCATGGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactg                      <  1:637461‑M1/149‑128 (MQ=255)                                                                                                                               gagtaccagtgaAGGTAACAAAcatggcgaccatctgacaacgtagcgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcacattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactg                      <  2:2076838‑M1/144‑128 (MQ=255)                                                                                                                                aCCGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactgg                     <  1:108660‑M1/149‑129 (MQ=255)                                                                                                                                 ccGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactgga                    <  1:760412‑M1/149‑130 (MQ=255)                                                                                                                                 ccGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactgga                    >  2:1183069‑M1/1‑20 (MQ=255)                                                                                                                                 ccGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactgga                    <  2:2057097‑M1/149‑130 (MQ=255)                                                                                                                                  cGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaacctgctgaaacactggat                   >  1:880678‑M1/1‑19 (MQ=255)                                                                                                                                  cGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccggtgaaactgctgaaaaactgggt                   >  1:668840‑M1/1‑19 (MQ=255)                                                                                                                                  cGCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccggggtgccgttgaaactgctgaaaaactggat                   >  1:954667‑M1/1‑19 (MQ=255)                                                                                                                                   gCCATTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatg                  >  1:1460661‑M1/1‑18 (MQ=255)                                                                                                                                     cactgaaGGTAACAAAcatggcgaccatctgcgaacatcccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgct                <  1:857851‑M1/146‑134 (MQ=255)                                                                                                                                      aTTGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctc               >  1:1673765‑M1/1‑15 (MQ=255)                                                                                                                                       ttGAAGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgcaactgctgaaaaactggatgctcc              >  2:1088475‑M1/1‑14 (MQ=255)                                                                                                                                          aaGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcg                                                          >  2:1348893‑M1/1‑11 (MQ=255)                                                                                                                                          aaGGTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcg                                                          <  1:1348893‑M1/102‑92 (MQ=255)                                                                                                                                             gTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagc                                                                                                          >  1:361412‑M1/1‑8 (MQ=255)                                                                                                                                             gTAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagc                                                                                                          <  2:361412‑M1/51‑44 (MQ=255)                                                                                                                                              tAACAAAcatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaaaaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgcaaaactggatgctccgctgatc       >  1:257018‑M1/1‑7 (MQ=255)                                                                                                                                               aacaaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatcg      >  1:290721‑M1/1‑6 (MQ=255)                                                                                                                                                acaaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcga                                                                                                  >  1:1627501‑M1/1‑5 (MQ=255)                                                                                                                                                acaaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcga                                                                                                  <  2:1627501‑M1/56‑52 (MQ=255)                                                                                                                                                 caaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatcgtg    >  2:125992‑M1/1‑4 (MQ=255)                                                                                                                                                 caaacagggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatcgtg    <  2:181000‑M1/149‑146 (MQ=255)                                                                                                                                                  aaacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatcgtgg   >  1:1204084‑M1/1‑3 (MQ=255)                                                                                                                                                   aacatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcggtatgccgtggtgccgttgaaactgctgaaaaactggatgctccgctgatcgtggt  >  1:1430858‑M1/1‑2 (MQ=255)                                                                                                                                                    acatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcgg                                                         >  2:735612‑M1/1‑1 (MQ=255)                                                                                                                                                    acatggcgaccatctgcgaacgtaccgaccgcgtgatgaacagccgtctcgagttcaacaatgacaaccgtaaactgcgcattaccgaagcgg                                                         <  1:735612‑M1/93‑93 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCT  >  NC_000913_3_pae_tpiA/1755973‑1756267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑