Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,756,121	Δ557 bp	coding (424‑980/1413 nt)	pykF →	pyruvate kinase I

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1756121	1756677	557	98 [0]	[0] 98	pykF	pyruvate kinase I

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1756120	0 (0.000)	98 (0.830)	80/280	0.4	100%	coding (423/1413 nt)	pykF	pyruvate kinase I
	?	NC_000913_3_pae_tpiA	1756678 =	0 (0.000)					coding (981/1413 nt)	pykF	pyruvate kinase I

CCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGT  >  NC_000913_3_pae_tpiA/1756530‑1756824                                                                                                                                                     |                                                                                                                                                   tttcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaac                                                                                                                                                    >  1:123600‑M2/149‑149 (MQ=255)   tcaccactgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCAt                                                                                                                                                  <  2:1089067‑M2/3‑1 (MQ=255)     accactgataaatctgttatcggcaacagcgaaatggttgcggtaacggatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATgg                                                                                                                                                >  1:106757‑M2/145‑149 (MQ=255)      ccactgataaatctgttatcggccaccgctaaatggttgcggttccccgccaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGc                                                                                                                                               <  1:1008573‑M2/6‑1 (MQ=255)         ctgataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGAc                                                                                                                                            <  1:1953159‑M2/9‑1 (MQ=255)           gataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCa                                                                                                                                          >  1:402534‑M2/139‑149 (MQ=255)            ataaatctgttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCAt                                                                                                                                         <  2:868623‑M2/12‑1 (MQ=255)                     ttatcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACg                                                                                                                                >  2:1247270‑M2/129‑149 (MQ=255)                       atcggcaacagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtaatggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTa                                                                                                                              >  2:760412‑M2/127‑149 (MQ=255)                              acagcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccggactgattgacgatggtctgatcggtatggaggttaccgccattgagtgtaacaaaGATGGCTACCATCTGCGAAGGTaccggccg                                                                                                                       >  1:181000‑M2/120‑149 (MQ=255)                               cagcgaaatggttgcggtaacgtatgatggtttcactattgacctgtctgttggcaacaccgtactggttgacgatggtctgctcggtatggaagttggattgattgaaggtaacaaaCATGGTGACTATCTGCGAACGTACCGACcgc                                                                                                                      <  2:347893‑M2/31‑1 (MQ=255)                                agcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACcgcg                                                                                                                     >  1:194218‑M2/118‑149 (MQ=255)                                 gcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGt                                                                                                                    <  1:786126‑M2/33‑1 (MQ=255)                                 gcgaaatggttgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCAACCATCAACCAACGTACAGACCACGt                                                                                                                    >  1:407657‑M2/117‑149 (MQ=255)                                    aaatggttggggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGtgat                                                                                                                 <  1:3813‑M2/36‑1 (MQ=255)                                    aaacggtcgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgagggtctgatcggtctggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGtgat                                                                                                                 <  1:466904‑M2/36‑1 (MQ=255)                                           tgcggtaacgtatgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACAGCGTGATGACCAGc                                                                                                          >  2:577444‑M2/107‑149 (MQ=255)                                                 aaagtatgacggtttcattactaacctgtctgttggcaacaccggattggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCCAACGTACCGACCGCGTGATGAACAGCCGtctc                                                                                                    <  2:481671‑M2/49‑1 (MQ=255)                                                   cgtatgaaggtttcactactgacctgtctggtggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCAGGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGa                                                                                                  <  2:346042‑M2/51‑1 (MQ=255)                                                      atgaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGtt                                                                                               <  1:1076919‑M2/54‑1 (MQ=255)                                                        gaaggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTCAGAACGTACTGACCGGGTGCTGAACAGCCGtctcgatgtga                                                                                             >  2:1449494‑M2/94‑144 (MQ=255)                                                          aggtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaac                                                                                           >  1:1331914‑M2/92‑149 (MQ=255)                                                            gtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGc                                                                                                                                               >  2:564898‑M2/90‑95 (MQ=255)                                                            gtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGc                                                                                                                                               <  1:564898‑M2/6‑1 (MQ=255)                                                            gtttcactactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGGGTGATGAACAGCGGTCTCGGGTTccacaa                                                                                         >  2:425815‑M2/90‑149 (MQ=255)                                                            gtttcactactgacctgtctgttggcaacaccgtactggttgacgatgatctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGttcaacca                                                                                         >  2:1874542‑M2/90‑147 (MQ=255)                                                            gtttcactactgacctgtctgttggcaacaccgtactggtcgacgatggtctgatcgggttggcagttacccccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATAAACAGCCGTCTCGAGTTcaacaa                                                                                         <  2:1920724‑M2/60‑1 (MQ=255)                                                            gtttcactactgacctgtctcttggcaacaccatactggttgacgatgggctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGAGAACGTAACGACCGCGTGATGAACAGCCGTGTCGATATcaacaa                                                                                         >  2:580120‑M2/90‑149 (MQ=255)                                                                  ctactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTa                                                                                                                              >  2:342854‑M2/84‑106 (MQ=255)                                                                  ctactgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTa                                                                                                                              <  1:342854‑M2/23‑1 (MQ=255)                                                                  ctactgacctgtctgttggcaacaccgtacgggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGTACAGCCGTCTCGAGTTCAACAATGACaa                                                                                   <  1:1670248‑M2/66‑1 (MQ=255)                                                                   cactgacctgtctgttggcaacaccgtactggttgaagatggtctggtcggtatggaatttaccgccattgaaggtaacaaaCAGGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAAc                                                                                  <  2:1999020‑M2/67‑1 (MQ=255)                                                                    actgatctggctgttggcaacaccggactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAAcc                                                                                 <  2:2033619‑M2/68‑1 (MQ=255)                                                                    actgacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCTAGTTCAACAATGACAAcc                                                                                 <  2:247877‑M2/68‑1 (MQ=255)                                                                       gacctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTa                                                                              >  2:1342743‑M2/79‑149 (MQ=255)                                                                         cctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTaaa                                                                            <  1:891556‑M2/73‑1 (MQ=255)                                                                         cctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTaaa                                                                            >  1:2021953‑M2/77‑149 (MQ=255)                                                                          ctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACACTGACAACCgtaagc                                                                           >  2:1729624‑M2/76‑147 (MQ=255)                                                                          ctgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAAc                                                                           >  1:1579199‑M2/76‑149 (MQ=255)                                                                           tgtctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACt                                                                          <  1:1536988‑M2/75‑1 (MQ=255)                                                                              ctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTATCGACCCCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGtaaaccacg                                                                       >  2:1010055‑M2/72‑145 (MQ=255)                                                                              ctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTgcg                                                                       >  1:1576807‑M2/72‑149 (MQ=255)                                                                              ctgttggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTgcg                                                                       <  2:1617227‑M2/78‑1 (MQ=255)                                                                                  tggcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCAtt                                                                   <  1:349494‑M2/82‑1 (MQ=255)                                                                                    gcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGGCCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAc                                                                 >  1:1970539‑M2/66‑149 (MQ=255)                                                                                    gcaacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAc                                                                 <  1:1414057‑M2/84‑1 (MQ=255)                                                                                      aacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCg                                                               >  2:1070303‑M2/64‑149 (MQ=255)                                                                                      aacaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGCTCAACAATCACAACCGTAAACTCCGCATTaccc                                                               >  2:834490‑M2/64‑148 (MQ=255)                                                                                       acaccgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGa                                                              >  2:1426508‑M2/63‑149 (MQ=255)                                                                                        caccgtactggttgacgatgggctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGaa                                                             <  2:1596433‑M2/88‑1 (MQ=255)                                                                                         accgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAg                                                            >  2:511496‑M2/61‑149 (MQ=255)                                                                                         accgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAg                                                            >  1:1179642‑M2/61‑149 (MQ=255)                                                                                         accgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAg                                                            >  1:1064712‑M2/61‑149 (MQ=255)                                                                                           cgtactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCg                                                          >  2:108660‑M2/59‑149 (MQ=255)                                                                                             tactggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGt                                                        >  1:671471‑M2/57‑149 (MQ=255)                                                                                              actggttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAAAGATAATATCCTTAAACTGCGCATTACCGAAGCGGTa                                                       <  1:1553100‑M2/94‑1 (MQ=255)                                                                                                  gttgacgatggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGcc                                                   <  2:1064712‑M2/98‑1 (MQ=255)                                                                                                     gacgatggtctgatcgggatggaagttaccgccattgaatgtagcaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtg                                                <  2:1841102‑M2/101‑1 (MQ=255)                                                                                                           ggtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCg                                          >  1:1647112‑M2/43‑149 (MQ=255)                                                                                                            gtctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGt                                         >  1:676900‑M2/42‑149 (MQ=255)                                                                                                             tctgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGtt                                        <  1:12503‑M2/109‑1 (MQ=255)                                                                                                               tgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACATTGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGa                                      >  1:1781376‑M2/39‑149 (MQ=255)                                                                                                               tgatcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGa                                      <  1:1247270‑M2/111‑1 (MQ=255)                                                                                                                  tcggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCTCATTACCGTAGCGGTATGCCGTGGTGCCGTTGAAAc                                   >  2:796135‑M2/36‑149 (MQ=255)                                                                                                                    ggtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATTTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActg                                 <  2:209066‑M2/116‑1 (MQ=255)                                                                                                                     gtatggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgc                                >  1:1566024‑M2/33‑149 (MQ=255)                                                                                                                      tagggaagttaccgccattgaaggcaacaaaCCTGGCGACCATCTGCGAACGTACCGACCGCATGATGACCAGCCGTCACGAGTTCAACAATGCCAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGCCGTGCCGTTAAACctgct                               >  2:865266‑M2/32‑149 (MQ=255)                                                                                                                        tggaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGACGCGGTATGCCATGGTGCCGTTGAAACTGCTGa                             >  2:1199480‑M2/30‑149 (MQ=255)                                                                                                                          gaagttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaaa                           >  1:900334‑M2/28‑149 (MQ=255)                                                                                                                              ttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                       >  2:161539‑M2/24‑149 (MQ=255)                                                                                                                              ttaccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAACGGTATGCCGTGGTTCCCTTGAACatgctgacagatt                       >  2:2035932‑M2/24‑143 (MQ=255)                                                                                                                               taccgccatggaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTg                      <  1:637461‑M2/127‑1 (MQ=255)                                                                                                                               gagtaccagtgaaggtaacaaaCATGGCGACCATCTGACAACGTAGCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCACATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTg                      <  2:2076838‑M2/127‑1 (MQ=255)                                                                                                                                accgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTgg                     <  1:108660‑M2/128‑1 (MQ=255)                                                                                                                                 ccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGa                    <  1:760412‑M2/129‑1 (MQ=255)                                                                                                                                 ccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGa                    >  2:1183069‑M2/21‑149 (MQ=255)                                                                                                                                 ccgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGa                    <  2:2057097‑M2/129‑1 (MQ=255)                                                                                                                                  cgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAACCTGCTGAAACACTGGAt                   >  1:880678‑M2/20‑149 (MQ=255)                                                                                                                                  cgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGGTGAAACTGCTGAAAAActgggt                   >  1:668840‑M2/20‑147 (MQ=255)                                                                                                                                  cgccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGGGGTGCCGTTGAAACTGCTGAAAAACTGGAt                   >  1:954667‑M2/20‑149 (MQ=255)                                                                                                                                   gccattgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATg                  >  1:1460661‑M2/19‑149 (MQ=255)                                                                                                                                     cactgaaggtaacaaaCATGGCGACCATCTGCGAACATCCCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCt                <  1:857851‑M2/133‑1 (MQ=255)                                                                                                                                      attgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTc               >  1:1673765‑M2/16‑149 (MQ=255)                                                                                                                                       ttgaaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGCAACTGCTGAAAAACTGGATGCTcc              >  2:1088475‑M2/15‑149 (MQ=255)                                                                                                                                          aaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCg                                                          >  2:1348893‑M2/12‑102 (MQ=255)                                                                                                                                          aaggtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCg                                                          <  1:1348893‑M2/91‑1 (MQ=255)                                                                                                                                             gtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGc                                                                                                          >  1:361412‑M2/9‑51 (MQ=255)                                                                                                                                             gtaacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGc                                                                                                          <  2:361412‑M2/43‑1 (MQ=255)                                                                                                                                              taacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAAAAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGCAAAACTGGATGCTCCGCTGATc       >  1:257018‑M2/8‑149 (MQ=255)                                                                                                                                               aacaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCg      >  1:290721‑M2/7‑149 (MQ=255)                                                                                                                                                acaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGa                                                                                                  >  1:1627501‑M2/6‑56 (MQ=255)                                                                                                                                                acaaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGa                                                                                                  <  2:1627501‑M2/51‑1 (MQ=255)                                                                                                                                                 caaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTg    >  2:125992‑M2/5‑149 (MQ=255)                                                                                                                                                 caaaCAGGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTg    <  2:181000‑M2/145‑1 (MQ=255)                                                                                                                                                  aaaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTgg   >  1:1204084‑M2/4‑149 (MQ=255)                                                                                                                                                   aaCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGt  >  1:1430858‑M2/3‑149 (MQ=255)                                                                                                                                                    aCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCgg                                                         >  2:735612‑M2/2‑93 (MQ=255)                                                                                                                                                    aCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCgg                                                         <  1:735612‑M2/92‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGT  >  NC_000913_3_pae_tpiA/1756530‑1756824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑