breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 264,098 G→T L50L (CTC→CTA ykfH ← uncharacterized protein
RA 482,230 G→A P725L (CCG→CTG)  acrB ← multidrug efflux system protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,106,741 A→T S162S (TCT→TCA wbbH ← O‑antigen polymerase
RA 2,111,423 G→T A185E (GCG→GAG)  rfbD ← dTDP‑L‑rhamnose synthase, NAD(P)‑dependent dTDP‑4‑dehydrorhamnose reductase subunit
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,679,525 G→T A1074D (GCT→GAT)  yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,795,396 C→A Q382K (CAG→AAG)  gabP → gamma‑aminobutyrate transporter
RA 2,817,170 C→A E112* (GAA→TAA)  yqaB ← fructose‑1‑P and 6‑phosphogluconate phosphatase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,810 Δ1 bp intergenic (‑42/+24) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 4,296,411 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,604,746 C→A A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_vch_tpiA 366237 366398 162 6 [4] [5] 7 [lacZ] [lacZ]
* * ÷ NC_000913_3_vch_tpiA 1196270 1211448 15179 6 [5] [5] 6 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfI,ymfJ,cohE,croE,ymfL,ymfM,oweE,aaaE,ymfR,beeE,jayE,ymfQ,stfP,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913_3_vch_tpiA 3423775–3424542 3424542 1–768 6 [5] [5] 6 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_tpiA 1096961 =13 (0.520)4 (0.240)
+47 bp
3/192 2.4 31.5% noncoding (1096961/4641682 nt) repeat_region repeat region
?NC_000913_3_vch_tpiA 1097243 = NA (NA)noncoding (1097243/4641682 nt) repeat_region repeat region
* ? NC_000913_3_vch_tpiA = 12994980 (0.000)20 (0.830) 18/278 0.4 100% noncoding (1299498/4641682 nt) repeat_region repeat region
?NC_000913_3_vch_tpiA 1300698 = 0 (0.000)noncoding (1300698/4641682 nt) repeat_region repeat region
* ? NC_000913_3_vch_tpiA 1552101 =NA (NA)7 (0.310) 7/262 1.8 NA noncoding (31/97 nt) RIP120 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP120 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913_3_vch_tpiA 1552249 = NA (NA)noncoding (1/97 nt) RIP121 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP121 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913_3_vch_tpiA 2161648 =15 (0.600)5 (0.200) 5/282 2.6 23.4% noncoding (2161648/4641682 nt) repeat_region repeat region
?NC_000913_3_vch_tpiA 2161675 = 18 (0.730)noncoding (2161675/4641682 nt) repeat_region repeat region
* ? NC_000913_3_vch_tpiA = 3674455NA (NA)4 (0.170) 4/276 2.9 NA noncoding (75/93 nt) REP263 (repetitive extragenic palindromic) element; contains 2 REP sequences REP263 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913_3_vch_tpiA = 3674459 NA (NA)noncoding (79/93 nt) REP263 (repetitive extragenic palindromic) element; contains 2 REP sequences REP263 (repetitive extragenic palindromic) element; contains 2 REP sequences