breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorseTMglyKat_S6_L001_R1_001.good.fq1,336,307273,382,221100.0%204.6 bases240 bases99.0%
errorseTMglyKat_S6_L001_R2_001.good.fq1,336,307274,348,383100.0%205.3 bases240 bases94.5%
total2,672,614547,730,604100.0%204.9 bases240 bases96.8%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0212141,869,612266.814.6100.0%Thermotoga maritima MSB8, complete genome.
total1,869,612100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000010086
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000150
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.039

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0212140.66135

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.8
R3.3.1

Execution Times

stepstartendelapsed
Read and reference sequence file input14:55:53 03 Mar 201814:56:48 03 Mar 201855 seconds
Read alignment to reference genome14:56:48 03 Mar 201815:01:07 03 Mar 20184 minutes 19 seconds
Preprocessing alignments for candidate junction identification15:01:07 03 Mar 201815:02:19 03 Mar 20181 minute 12 seconds
Preliminary analysis of coverage distribution15:02:19 03 Mar 201815:04:36 03 Mar 20182 minutes 17 seconds
Identifying junction candidates15:04:36 03 Mar 201815:05:59 03 Mar 20181 minute 23 seconds
Re-alignment to junction candidates15:05:59 03 Mar 201815:07:13 03 Mar 20181 minute 14 seconds
Resolving best read alignments15:07:13 03 Mar 201815:09:11 03 Mar 20181 minute 58 seconds
Creating BAM files15:09:11 03 Mar 201815:11:03 03 Mar 20181 minute 52 seconds
Tabulating error counts15:11:03 03 Mar 201815:12:24 03 Mar 20181 minute 21 seconds
Re-calibrating base error rates15:12:24 03 Mar 201815:12:26 03 Mar 20182 seconds
Examining read alignment evidence15:12:26 03 Mar 201815:27:43 03 Mar 201815 minutes 17 seconds
Polymorphism statistics15:27:43 03 Mar 201815:27:44 03 Mar 20181 second
Output15:27:44 03 Mar 201815:28:20 03 Mar 201836 seconds
Total 32 minutes 27 seconds