breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC 1,110 +66 bp intergenic (+1035/+2045) TMARI_RS09660 → / ← TMARI_RS00005 hypothetical protein/IGHMBP2 family helicase
RA 8,781 C→T C558Y (TGT→TAT)  TMARI_RS00030 ← NADH‑quinone oxidoreductase subunit NuoF
RA 51,724 G→A L644L (CTC→CTT TMARI_RS00255 ← xylan alpha‑1,2‑glucuronosidase
RA 70,289 G→A D116N (GAT→AAT)  TMARI_RS00325 → mannonate dehydratase
RA 153,436 C→T intergenic (‑8/+10) TMARI_RS00715 ← / ← TMARI_RS00720 ribosome silencing factor/glutamine‑‑fructose‑6‑phosphate aminotransferase
RA 174,525 C→T V182V (GTC→GTT TMARI_RS00820 → leucine‑‑tRNA ligase
RA 189,999 C→T noncoding (1033/1564 nt) TMARI_RS00875 → 16S ribosomal RNA
RA 252,862 C→T G171G (GGG→GGA TMARI_RS01220 ← UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑LD‑ lysine ligase
RA 253,934 G→A D165N (GAT→AAT)  TMARI_RS01225 → hypothetical protein
RA 254,907 C→T A295V (GCA→GTA)  TMARI_RS01230 → glucose‑1‑phosphate adenylyltransferase subunit GlgD
RA 255,452 C→T A102V (GCC→GTC)  TMARI_RS01235 → glucose‑1‑phosphate adenylyltransferase
RA 263,426 C→T A154V (GCG→GTG)  TMARI_RS01285 → DNA‑protecting protein DprA
RA 292,337 C→T P288S (CCT→TCT)  TMARI_RS01420 → sugar ABC transporter substrate‑binding protein
RA 323,757 C→T P242S (CCT→TCT)  TMARI_RS01555 → ABC transporter ATP‑binding protein
RA 324,273 G→A D92N (GAT→AAT)  TMARI_RS01560 → endoglucanase
RA 329,640 C→T R520K (AGA→AAA)  TMARI_RS01575 ← alpha‑xylosidase
RA 334,236 C→T L233L (CTC→CTT TMARI_RS01585 → beta‑galactosidase
RA 374,924 C→T S31N (AGC→AAC)  TMARI_RS01815 ← TolC family protein
RA 380,678 C→T F99F (TTC→TTT TMARI_RS01855 → fibronectin‑binding domain‑containing protein
RA 391,330 C→T R236R (AGG→AGA TMARI_RS01900 ← AcrB/AcrD/AcrF family protein
RA 423,580 C→T T29I (ACT→ATT)  TMARI_RS02045 → P‑II family nitrogen regulator
RA 441,508 +G coding (365/1095 nt) gldA ← glycerol dehydrogenase
RA 453,685 G→A intergenic (‑55/‑192) TMARI_RS02190 ← / → TMARI_RS02195 carbohydrate ABC transporter substrate‑binding protein/pectate trisaccharide‑lyase
RA 464,696 C→T H310H (CAC→CAT TMARI_RS02240 → DUF2088 domain‑containing protein
RA 529,260 C→T M94I (ATG→ATA TMARI_RS02570 ← ABC transporter ATP‑binding protein
RA 531,887 Δ1 bp intergenic (‑320/‑355) TMARI_RS02580 ← / → TMARI_RS02585 ABC transporter permease/molecular chaperone GroES
RA 566,333 G→A V134M (GTG→ATG)  TMARI_RS02765 → fumarate hydratase
RA 596,931 A→T M26K (ATG→AAG)  TMARI_RS02905 ← Rrf2 family transcriptional regulator
RA 622,429 C→T T107M (ACG→ATG)  TMARI_RS03015 → rod shape‑determining protein
RA 653,212 T→C R25R (AGA→AGG TMARI_RS03175 ← glycosyltransferase family 4 protein
RA 692,396 C→T E232E (GAG→GAA TMARI_RS03390 ← ribonuclease J
RA 700,621 C→T P516S (CCC→TCC)  TMARI_RS03435 → flagellar hook‑length control protein FliK
RA 712,512 (A)7→8 coding (1061/1437 nt) TMARI_RS03505 → DNA polymerase III subunit gamma/tau
RA 712,650 +A coding (1199/1437 nt) TMARI_RS03505 → DNA polymerase III subunit gamma/tau
RA 725,314 C→T V283V (GTG→GTA TMARI_RS03590 ← cinA‑like protein
RA 732,714 C→T P79L (CCA→CTA)  TMARI_RS03625 → MarR family transcriptional regulator
RA 771,217 C→T D42N (GAT→AAT)  TMARI_RS03825 ← tRNA (adenine‑N1)‑methyltransferase
RA 775,454 C→T E144E (GAG→GAA TMARI_RS03850 ← alpha‑glucosidase/alpha‑galactosidase
RA 886,742 C→T D109N (GAC→AAC)  TMARI_RS04415 ← glucose‑1‑phosphate thymidylyltransferase
RA 927,801 G→A I15I (ATC→ATT TMARI_RS04645 ← flagellar biosynthesis protein FlhA
RA 961,214 G→A K317K (AAG→AAA TMARI_RS04830 → hypothetical protein
RA 1,008,986 C→T L738F (CTT→TTT)  TMARI_RS05055 → DUF3883 domain‑containing protein
RA 1,050,568 C→T G155G (GGG→GGA TMARI_RS05270 ← mannose‑1‑phosphate guanylyltransferase
RA 1,079,864 C→T G187S (GGT→AGT)  TMARI_RS05430 ← ABC transporter permease
RA 1,107,515 G→A R372K (AGA→AAA)  TMARI_RS05560 → histidine‑‑tRNA ligase
RA 1,162,954 C→T T180T (ACG→ACA TMARI_RS05860 ← ABC transporter permease
RA 1,187,604 C→T R24R (CGG→CGA TMARI_RS05970 ← ABC transporter substrate‑binding protein
RA 1,236,482 G→A G62R (GGA→AGA)  TMARI_RS06190 → NADH dehydrogenase
RA 1,250,396 C→T G427E (GGA→GAA)  TMARI_RS06255 ← ABC transporter substrate‑binding protein
RA 1,339,657 G→A G319D (GGT→GAT)  TMARI_RS06710 → ABC transporter ATP‑binding protein
RA 1,351,908 A→G A207A (GCT→GCC TMARI_RS06775 ← hypothetical protein
RA 1,446,537 G→A G127D (GGT→GAT)  TMARI_RS07295 → glycerol kinase
RA 1,468,583 G→A A195V (GCG→GTG)  TMARI_RS07400 ← RNA polymerase sigma factor SigA
RA 1,530,195 T→G L24R (CTC→CGC)  TMARI_RS07850 → B12‑binding domain‑containing radical SAM protein
RA 1,569,199 C→A S246Y (TCT→TAT)  TMARI_RS08065 → peptide chain release factor 2
RA 1,645,038 C→T R111K (AGA→AAA)  TMARI_RS08475 ← DNA‑binding response regulator
RA 1,651,040 G→A S198S (TCC→TCT TMARI_RS08520 ← class I SAM‑dependent methyltransferase
RA 1,677,498 G→A M16I (ATG→ATA TMARI_RS08700 → HD‑GYP domain‑containing protein
RA 1,718,805 G→A R175K (AGA→AAA)  TMARI_RS08915 → haloacid dehalogenase
RA 1,755,576 G→A intergenic (‑37/+25) TMARI_RS09105 ← / ← TMARI_RS09110 ribosome maturation factor/tRNA‑Ser
RA 1,784,731 G→A P265S (CCA→TCA)  TMARI_RS09265 ← type III‑A CRISPR‑associated RAMP protein Csm4
RA 1,806,077 G→A H62Y (CAT→TAT)  TMARI_RS09375 ← IS200/IS605 family transposase
MC JC 1,814,273 (CCCT)2→1 intergenic (‑48/+60) TMARI_RS09830 ← / ← TMARI_RS09405 DUF4896 domain‑containing protein/maltose/maltodextrin ABC transporter substrate‑binding protein MalE
MC JC 1,815,540 Δ28 bp intergenic (‑23/‑201) TMARI_RS09405 ← / → TMARI_RS09410 maltose/maltodextrin ABC transporter substrate‑binding protein MalE/alpha‑amylase
RA 1,839,693 G→A G80R (GGA→AGA)  TMARI_RS09490 → DUF4382 domain‑containing protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_021214 1341068–1341894 1351798–1350987 9094–10731 88 [81] [85] 86 [TMARI_RS06725]–[TMARI_RS06775] [TMARI_RS06725], TMARI_RS06730, TMARI_RS06735, TMARI_RS06740, TMARI_RS09805, TMARI_RS06745, TMARI_RS06750, TMARI_RS06755, TMARI_RS06760, TMARI_RS06765, TMARI_RS06770, [TMARI_RS06775]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_021214 206152 =272 (0.970)135 (0.550) 80/358 1.3 33.9% noncoding (77/77 nt) TMARI_RS00975 tRNA‑Arg
?NC_021214 = 206320 289 (1.170)intergenic (‑11/+1) TMARI_RS00980/TMARI_RS00985 tRNA‑His/tRNA‑Arg