breseq version 0.32.0 revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_021214 | 318,288 | 0 | C | T | 64.7% | 102.3 / 122.3 | 118 | F11F (TTC→TTT) | TMARI_RS01535 | peptide ABC transporter substrate‑binding protein |
* | NC_021214 | 809,212 | 0 | C | T | 42.2% | 140.6 / 296.9 | 237 | pseudogene (292/790 nt) | TMARI_RS04015 | Maritimacin |
* | NC_021214 | 1,800,294 | 0 | G | A | 29.8% | 486.2 / 251.2 | 302 | Y209Y (TAC→TAT) | TMARI_RS09340 | hypothetical protein |
* | NC_021214 | 358,524 | 0 | T | A | 27.7% | 345.3 / 39.1 | 141 | intergenic (‑106/‑79) | TMARI_RS01730/TMARI_RS01735 | hypothetical protein/DUF2089 domain‑containing protein |
* | NC_021214 | 358,511 | 0 | G | A | 27.5% | 278.3 / 50.0 | 142 | intergenic (‑93/‑92) | TMARI_RS01730/TMARI_RS01735 | hypothetical protein/DUF2089 domain‑containing protein |
* | NC_021214 | 26,468 | 0 | C | A | 23.9% | 612.5 / 52.5 | 216 | E32* (GAG→TAG) | TMARI_RS00115 | ABC transporter ATP‑binding protein |
* | NC_021214 | 693,623 | 0 | G | T | 23.4% | 299.8 / 63.9 | 145 | A237A (GCC→GCA) | TMARI_RS03400 | cysteine synthase A |
* | NC_021214 | 693,637 | 0 | C | T | 22.9% | 287.6 / 44.0 | 144 | E233K (GAG→AAG) | TMARI_RS03400 | cysteine synthase A |
* | NC_021214 | 602,920 | 0 | T | G | 22.6% | 439.4 / 13.6 | 187 | V382G (GTT→GGT) L17V (TTG→GTG) | TMARI_RS02930 TMARI_RS02935 | DegT/DnrJ/EryC1/StrS aminotransferase family protein apolipoprotein N‑acyltransferase |
* | NC_021214 | 825,745 | 0 | C | T | 22.5% | 263.7 / 20.3 | 122 | P81S (CCC→TCC) | TMARI_RS04110 | histidinol‑phosphatase |
* | NC_021214 | 358,545 | 0 | G | T | 22.2% | 412.7 / 47.9 | 145 | intergenic (‑127/‑58) | TMARI_RS01730/TMARI_RS01735 | hypothetical protein/DUF2089 domain‑containing protein |
* | NC_021214 | 693,643 | 0 | T | A | 22.2% | 352.5 / 49.0 | 155 | T231S (ACC→TCC) | TMARI_RS03400 | cysteine synthase A |
* | NC_021214 | 1,284,753 | 0 | C | A | 22.2% | 243.1 / 17.8 | 90 | Q407K (CAA→AAA) | TMARI_RS06405 | ATP‑binding protein |
* | NC_021214 | 1,777,055 | 0 | A | T | 22.1% | 385.1 / 36.2 | 150 | N62K (AAT→AAA) | TMARI_RS09225 | type I‑B CRISPR‑associated protein Cas5 |
* | NC_021214 | 1,239,628 | 0 | T | G | 22.0% | 421.8 / 33.0 | 179 | C97G (TGT→GGT) | TMARI_RS06210 | oxidoreductase |
* | NC_021214 | 622,253 | 0 | G | A | 21.6% | 449.4 / 29.8 | 186 | A48A (GCG→GCA) | TMARI_RS03015 | rod shape‑determining protein |
* | NC_021214 | 910,896 | 0 | A | G | 21.6% | 258.7 / 18.4 | 113 | G27G (GGA→GGG) | TMARI_RS04545 | thiamine diphosphokinase |
* | NC_021214 | 1,869,446 | 0 | G | T | 20.9% | 565.5 / 43.0 | 202 | intergenic (+58/‑53) | TMARI_RS09635/TMARI_RS09660 | bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/hypothetical protein |
* | NC_021214 | 232,200 | 0 | G | A | 20.7% | 592.5 / 52.3 | 242 | R259Q (CGA→CAA) | TMARI_RS01120 | glycine‑‑tRNA ligase subunit beta |
* | NC_021214 | 1,232,534 | 0 | A | T | 20.6% | 464.3 / 35.2 | 186 | Y210F (TAC→TTC) | TMARI_RS06170 | cation:proton antiporter |
Marginal new junction evidence (lowest skew 10 of 83 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_021214 | = 697153 | 229 (0.820) | 12 (0.040) | 8/394 | 11.5 | 5.3% | coding (433/615 nt) | TMARI_RS03420 | hypothetical protein |
? | NC_021214 | = 697210 | 207 (0.760) | coding (376/615 nt) | TMARI_RS03420 | hypothetical protein | |||||
* | ? | NC_021214 | = 146936 | 260 (0.930) | 8 (0.030) | 8/392 | 11.5 | 2.5% | coding (878/1770 nt) | TMARI_RS00680 | bifunctional glutamine amidotransferase/anthranilate phosphoribosyltransferase |
? | NC_021214 | = 147166 | 372 (1.380) | coding (648/1770 nt) | TMARI_RS00680 | bifunctional glutamine amidotransferase/anthranilate phosphoribosyltransferase | |||||
* | ? | NC_021214 | 1071760 = | 277 (0.990) | 12 (0.040) | 7/398 | 12.0 | 4.1% | coding (667/1527 nt) | TMARI_RS05395 | FMN‑binding glutamate synthase family protein |
? | NC_021214 | 1071815 = | 294 (1.070) | coding (722/1527 nt) | TMARI_RS05395 | FMN‑binding glutamate synthase family protein | |||||
* | ? | NC_021214 | = 1774853 | 344 (1.230) | 8 (0.030) | 6/386 | 12.3 | 2.4% | coding (1762/2193 nt) | TMARI_RS09220 | CRISPR‑associated helicase/endonuclease Cas3 |
? | NC_021214 | = 1775154 | 339 (1.280) | coding (1461/2193 nt) | TMARI_RS09220 | CRISPR‑associated helicase/endonuclease Cas3 | |||||
* | ? | NC_021214 | = 1603565 | 231 (0.820) | 16 (0.060) | 6/386 | 12.3 | 6.7% | coding (245/258 nt) | TMARI_RS08270 | ATP synthase subunit C |
? | NC_021214 | = 1603631 | 227 (0.860) | coding (179/258 nt) | TMARI_RS08270 | ATP synthase subunit C | |||||
* | ? | NC_021214 | = 453135 | 405 (1.440) | 6 (0.020) | 6/392 | 12.4 | 1.6% | coding (496/1272 nt) | TMARI_RS02190 | carbohydrate ABC transporter substrate‑binding protein |
? | NC_021214 | = 453205 | 338 (1.250) | coding (426/1272 nt) | TMARI_RS02190 | carbohydrate ABC transporter substrate‑binding protein | |||||
* | ? | NC_021214 | 848026 = | 237 (0.840) | 6 (0.020) | 6/400 | 12.5 | 2.7% | coding (605/2367 nt) | TMARI_RS04210 | phenylalanine‑‑tRNA ligase subunit beta |
? | NC_021214 | 848219 = | 208 (0.760) | coding (798/2367 nt) | TMARI_RS04210 | phenylalanine‑‑tRNA ligase subunit beta | |||||
* | ? | NC_021214 | 334659 = | 376 (1.340) | 10 (0.040) | 6/398 | 12.5 | 2.8% | coding (1122/2019 nt) | TMARI_RS01585 | beta‑galactosidase |
? | NC_021214 | 334750 = | 336 (1.230) | coding (1213/2019 nt) | TMARI_RS01585 | beta‑galactosidase | |||||
* | ? | NC_021214 | 859217 = | 244 (0.870) | 6 (0.020) | 5/388 | 12.8 | 2.6% | coding (1544/1911 nt) | TMARI_RS04265 | DNA gyrase subunit B |
? | NC_021214 | 859476 = | 218 (0.820) | coding (1803/1911 nt) | TMARI_RS04265 | DNA gyrase subunit B | |||||
* | ? | NC_021214 | 645713 = | 247 (0.880) | 8 (0.030) | 5/388 | 12.8 | 3.2% | intergenic (‑224/‑113) | TMARI_RS03155/TMARI_RS03160 | ATPase/ATP‑binding protein |
? | NC_021214 | 645737 = | 243 (0.910) | intergenic (‑248/‑89) | TMARI_RS03155/TMARI_RS03160 | ATPase/ATP‑binding protein |