Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A3 F50 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) glycine(2mM),L-serine(),NaCl(0.5) 37 511145 E. coli K12 MG1655 ΔsdaA ΔsdaB ΔtdcG ΔglyA NC_000913_3.gb
A3 F50 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) glycine(2mM),L-serine(),NaCl(0.5) 37 511145 E. coli K12 MG1655 ΔsdaA ΔsdaB ΔtdcG ΔglyA NC_000913_3.gb
A4 F50 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) glycine(2mM),L-serine(),NaCl(0.5) 37 511145 E. coli K12 MG1655 ΔsdaA ΔsdaB ΔtdcG ΔglyA NC_000913_3.gb
A4 F50 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) glycine(2mM),L-serine(),NaCl(0.5) 37 511145 E. coli K12 MG1655 ΔsdaA ΔsdaB ΔtdcG ΔglyA NC_000913_3.gb
A5 F50 I1 R1 clonal M9 glucose(2g/L) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) glycine(2mM),L-serine(),NaCl(0.5) 37 511145 E. coli K12 MG1655 ΔsdaA ΔsdaB ΔtdcG ΔglyA NC_000913_3.gb
A5 F50 I2 R1 clonal M9 glucose(2g/L) NH4Cl(1) KH2PO4(3),Na2HPO4(6.8) MgSO4(0.24) CaCl2(0.1) glycine(2mM),L-serine(),NaCl(0.5) 37 511145 E. coli K12 MG1655 ΔsdaA ΔsdaB ΔtdcG ΔglyA NC_000913_3.gb