Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A0 F0 I1 R1 clonal BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A26 F2 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A26 F12 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A26 F36 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A27 F2 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A27 F12 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A27 F36 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A28 F2 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A28 F11 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A28 F35 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A29 F2 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A29 F11 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A29 F36 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A30 F2 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A30 F12 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]
A30 F36 I0 R1 population BM_ALE (NH4)2SO4(7.6) KH2PO4(15.7) MgSO4(0.5) None 37 316435 Nissle_1917 ['/var/data/NZ_MW240712.gbk', ['/var/data/NZ_CP058219.gbk', ['/var/data/NZ_CP022686.gbk', '/var/data/pHH-29_RBS_Adafermin.gbk']]]