Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F4 I5 R1	61	40.1	2005705	98.2%	1969602	131.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	5,660,005	A→C	H350P (CAC→CCC)	metK CDS (methionine adenosyltransferase) →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	5,660,005	0	A	C	100.0%	118.6 / NA	32	H350P (CAC→CCC)	metK CDS (methionine adenosyltransferase)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (20/12); total (20/12)

ATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTG  >  AE015451_phaM1/5659866‑5660144

aTTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCg                                                                                                                                         <  2:136765/144‑1 (MQ=255)

        ggCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATc                                                                                                                                  >  5:36666/1‑143 (MQ=255)

                              aTCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCAc                                                                                                           >  1:479803/1‑144 (MQ=255)

                                cAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTACCCCCGATGTATCAGGAAAACGCTGCCTACGGCCACt                                                                                                          >  6:82610/1‑143 (MQ=255)

                                                 gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCcccc                                                                                                                                          >  4:93312/1‑94 (MQ=255)

                                                 gCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCcccc                                                                                                                                          <  4:78445/94‑1 (MQ=255)

                                                       tctcCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCagaa                                                                                    <  2:422990/142‑1 (MQ=255)

                                                       tctcCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCATACTCCGCAGCagaa                                                                                    >  1:422990/1‑142 (MQ=255)

                                                        ctcCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTAt                                                                                                                                   >  3:94568/1‑94 (MQ=255)

                                                        ctcCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTAt                                                                                                                                   >  3:94567/1‑94 (MQ=255)

                                                         tcCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATc                                                                                                                                  <  3:17754/94‑1 (MQ=255)

                                                         tcCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATc                                                                                                                                  <  3:17753/94‑1 (MQ=255)

                                                             acgacAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCg                                                                            >  1:301563/1‑144 (MQ=255)

                                                             acgacAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCg                                                                            >  1:301562/1‑144 (MQ=255)

                                                                   aGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcgacg                                                                      >  2:277759/1‑144 (MQ=255)

                                                                   aGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcgacg                                                                      >  2:277758/1‑144 (MQ=255)

                                                                      tcatcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGaca                                                                   <  1:94807/144‑1 (MQ=255)

                                                                      tcatcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGaca                                                                   <  1:94808/144‑1 (MQ=255)

                                                                         tcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTa                                                                                                                  <  3:93312/94‑1 (MQ=255)

                                                                            aGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTCTCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGCCGAcaccttca                                                             >  2:18785/1‑144 (MQ=255)

                                                                            aGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttca                                                             >  2:18783/1‑144 (MQ=255)

                                                                                ggTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcaccac                                                         <  1:70755/144‑1 (MQ=255)

                                                                                    cgcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCg                                                                                                       >  3:179180/1‑94 (MQ=255)

                                                                                    cgcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCg                                                                                                       >  3:179179/1‑94 (MQ=255)

                                                                                    cgcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCg                                                                                                       >  3:179178/1‑94 (MQ=255)

                                                                                     gcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCgg                                                                                                      <  4:101885/94‑1 (MQ=255)

                                                                                        aGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCt                                                 >  1:381697/1‑144 (MQ=255)

                                                                                            cTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGa                                             <  1:360170/144‑1 (MQ=255)

                                                                                               cGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTTCg                                                                                            >  4:171652/1‑94 (MQ=255)

                                                                                                          cGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                                >  6:109759/1‑143 (MQ=255)

                                                                                                               gCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAg                          <  2:381697/144‑1 (MQ=255)

                                                                                                                         ccaTGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGacac                                                                  <  4:89054/94‑1 (MQ=255)

                                                                                                                                   ccTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCg       >  6:78522/1‑143 (MQ=255)

                                                                                                                                        tgcCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTg  >  5:83077/1‑143 (MQ=255)

ATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTG  >  AE015451_phaM1/5659866‑5660144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCC  >  AE015451_phaM1/5659866‑5660153

ATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATC                                                                                                                                           >  LH00444:90:22JGJLLT3:8:1212:1379:23127/1‑151 (MQ=60)

                       CATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCAC                                                                                                                    >  LH00444:58:22357VLT4:2:2308:12855:24351/1‑151 (MQ=60)

                                                TGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCATACTCCGCAGCAGAAGA                                                                                           >  LH00444:58:22357VLT4:2:2243:48582:1420/1‑151 (MQ=60)

                                                 GCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTAT                                                                                                                                            >  LH00444:62:22GH52LT3:8:1259:49200:22774/1‑101 (MQ=60)

                                                 GCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTAT                                                                                                                                            >  LH00444:62:22GH52LT3:8:1259:49182:22774/1‑101 (MQ=60)

                                                      GTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCG                                                                                     >  LH00444:58:22357VLT4:2:1493:3314:28539/1‑151 (MQ=60)

                                                      GTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCG                                                                                     >  LH00444:58:22357VLT4:2:1493:3331:28539/1‑151 (MQ=60)

                                                         TCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAAC                                                                                                                                    <  LH00444:62:22GH52LT3:8:1130:6927:28831/101‑1 (MQ=60)

                                                         TCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAAC                                                                                                                                    <  LH00444:62:22GH52LT3:8:1130:6918:28815/101‑1 (MQ=60)

                                                                      TCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCAA                                                                     <  LH00444:58:22357VLT4:2:1226:48550:17753/151‑1 (MQ=60)

                                                                      TCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCAA                                                                     <  LH00444:58:22357VLT4:2:1226:48566:17753/151‑1 (MQ=60)

                                                                         TCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCAC                                                                                                                    <  LH00444:62:22GH52LT3:8:1257:30578:17743/101‑1 (MQ=60)

                                                                             GCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCG                                                                                                                >  LH00444:62:22GH52LT3:8:2197:8665:15724/1‑101 (MQ=60)

                                                                             GCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCG                                                                                                                >  LH00444:62:22GH52LT3:8:2197:8674:15740/1‑101 (MQ=60)

                                                                             GCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCG                                                                                                                >  LH00444:62:22GH52LT3:8:2197:8693:15740/1‑101 (MQ=60)

                                                                                GGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACC                                                           <  LH00444:58:22357VLT4:2:1190:48251:28749/151‑1 (MQ=60)

                                                                                 GTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCT                                                          >  LH00444:58:22357VLT4:2:2188:26167:16549/1‑151 (MQ=60)

                                                                                            CTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACC                                               <  LH00444:58:22357VLT4:2:2163:19976:21942/151‑1 (MQ=60)

                                                                                                                                CGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTG           >  LH00444:90:22JGJLLT3:8:2161:8915:16542/1‑151 (MQ=60)

                                                                                                                                         GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCC  <  LH00444:58:22357VLT4:2:2234:6422:18398/151‑1 (MQ=60)

                                                                                                                                         GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCC  <  LH00444:58:22357VLT4:2:2234:6414:18384/151‑1 (MQ=60)

ATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCC  >  AE015451_phaM1/5659866‑5660153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`