Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F4 I1 R1 87 834.8 39728352 98.4% 39092698 137.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 5,660,005 A→C H350P (CAC→CCC)  metK CDS (methionine adenosyltransferase) →

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,660,0050AC99.9% 3215.2 / NA 772H350P (CAC→CCC) metK CDS (methionine adenosyltransferase)
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base C (277/493);  minor base G (0/1);  total (278/494)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 792 total aligned reads displayed.

GCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCG  >  AE015451_phaM1/5659870‑5660139
                                                                                                                                       |                                                                                                                                      
gCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCcccc                                                                                                                                     >  2:1221086/1‑139 (MQ=255)
gCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCcccc                                                                                                                                     >  2:14624870/1‑139 (MQ=255)
gCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCcccc                                                                                                                                     >  2:17990399/1‑139 (MQ=255)
 cGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGTTCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCg                                                                                                                                    >  2:7589687/1‑139 (MQ=255)
 cGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCg                                                                                                                                    <  1:2737262/139‑1 (MQ=255)
        cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGtctc                                                                                                                             >  2:7718033/1‑139 (MQ=255)
        cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATc                                                                                                                             >  2:9716414/1‑139 (MQ=255)
        cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATc                                                                                                                             >  2:15357065/1‑139 (MQ=255)
        cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATc                                                                                                                             >  2:17612645/1‑139 (MQ=255)
        cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATc                                                                                                                             >  2:10222386/1‑139 (MQ=255)
        cAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATc                                                                                                                             >  1:15015108/1‑139 (MQ=255)
           ccGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAgg                                                                                                                          <  1:13343551/139‑1 (MQ=255)
             gACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGaa                                                                                                                        >  2:448920/1‑139 (MQ=255)
             gACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGaa                                                                                                                        >  2:16292958/1‑139 (MQ=255)
              aCCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGaaa                                                                                                                       <  2:18139681/139‑1 (MQ=255)
               ccTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAAc                                                                                                                      <  2:3707649/139‑1 (MQ=255)
                   cATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCt                                                                                                                  >  1:19126320/1‑139 (MQ=255)
                   cATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCt                                                                                                                  >  2:8009142/1‑139 (MQ=255)
                       tcGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCt                                                                                                              <  1:9234908/139‑1 (MQ=255)
                        cGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTa                                                                                                             >  2:13548546/1‑139 (MQ=255)
                        cGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTACTGCCCCCGATGTATCAGGAAACCGCTGCCTa                                                                                                             >  1:6293172/1‑139 (MQ=255)
                        cGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCAGCTCCACCCGATGTATCAGGAAACCCCTGCCTa                                                                                                             >  2:18457623/1‑139 (MQ=255)
                            cAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGTAAACCGCTGCCTACGGc                                                                                                         <  1:15557155/139‑1 (MQ=255)
                            cAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGc                                                                                                         <  2:11212667/139‑1 (MQ=255)
                             aaCACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGcc                                                                                                        >  2:690289/1‑139 (MQ=255)
                              acacCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCa                                                                                                       <  2:19350318/139‑1 (MQ=255)
                              acacCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCa                                                                                                       <  2:4342766/139‑1 (MQ=255)
                              acacCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCa                                                                                                       <  1:4923296/139‑1 (MQ=255)
                                acCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACt                                                                                                     >  2:8649364/1‑139 (MQ=255)
                                 ccTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACtt                                                                                                    <  2:12027487/139‑1 (MQ=255)
                                 ccTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACtt                                                                                                    <  2:10904530/139‑1 (MQ=255)
                                 ccTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACtt                                                                                                    <  1:5252173/139‑1 (MQ=255)
                                  cTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTc                                                                                                   >  2:6585744/1‑139 (MQ=255)
                                    tgggTACCGGCAAGGTCTCCGACTACAAGATCATCCAGCTGGTGCCCGAGTGGTTCGACCTGCGTCCGTACGCCATCACCACCATGCGCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCAGTTCgg                                                                                                 <  2:2317212/137‑1 (MQ=255)
                                    tCGGTACCGCCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCgg                                                                                                 <  2:12989742/139‑1 (MQ=255)
                                      ggTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGcc                                                                                               <  2:19029539/139‑1 (MQ=255)
                                       gTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCg                                                                                              >  1:7291024/1‑139 (MQ=255)
                                       gTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCg                                                                                              >  1:10017429/1‑139 (MQ=255)
                                         aCCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTa                                                                                            <  1:11056705/139‑1 (MQ=255)
                                           cGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                          <  2:18799521/139‑1 (MQ=255)
                                           cGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                          <  1:15311316/139‑1 (MQ=255)
                                           cGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                          <  2:15058802/139‑1 (MQ=255)
                                           cGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACt                                                                                          <  2:10474771/139‑1 (MQ=255)
                                                     tcCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCaga                                                                                >  1:11663688/1‑139 (MQ=255)
                                                           gacAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTg                                                                          <  2:2680741/139‑1 (MQ=255)
                                                            acAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGt                                                                         <  1:1295210/139‑1 (MQ=255)
                                                                gATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGc                                                                     >  1:4105743/1‑139 (MQ=255)
                                                                gATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGc                                                                     >  1:17871451/1‑139 (MQ=255)
                                                                gATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGc                                                                     >  2:9208869/1‑139 (MQ=255)
                                                                 ctcttcCAGCTGGTGCGCCAGTGCATCCACCGCCGACCGTACCCCAACACCACCATGCTCGACCTCCTGCCCCCGATCTATCAGGAAACCGCTGCCTACGGCCACTTCGCCCGTACTCCGCAGCAGACGACAGTCGCcg                                                                    <  2:3381767/135‑1 (MQ=255)
                                                                 atcatcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcg                                                                    <  1:6545960/139‑1 (MQ=255)
                                                                  tcatcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcga                                                                   <  1:18049701/139‑1 (MQ=255)
                                                                   catcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcgac                                                                  <  2:1150765/139‑1 (MQ=255)
                                                                   catcCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGcgac                                                                  <  2:4012037/139‑1 (MQ=255)
                                                                        aGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGacac                                                             >  1:2320067/1‑139 (MQ=255)
                                                                          cTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                           <  2:11781512/139‑1 (MQ=255)
                                                                          cTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                           <  1:13963003/139‑1 (MQ=255)
                                                                          cTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                           <  2:12555983/139‑1 (MQ=255)
                                                                          cTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacct                                                           <  2:17436101/139‑1 (MQ=255)
                                                                           tGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcacctt                                                          <  1:8153742/139‑1 (MQ=255)
                                                                            ggTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttc                                                         <  1:18387399/139‑1 (MQ=255)
                                                                            ggTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttc                                                         <  1:15846048/139‑1 (MQ=255)
                                                                              tGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcac                                                       <  1:16810851/139‑1 (MQ=255)
                                                                              tGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcac                                                       >  1:4700765/1‑139 (MQ=255)
                                                                                cgcgAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcacca                                                     >  2:5760582/1‑139 (MQ=255)
                                                                                     gTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGAcaccttcaccaccttc                                                <  1:6766746/139‑1 (MQ=255)
                                                                                     ctGCTTCGACCTGCGTCCGGACGCCATCACCACCATGCTCGACCTGCTGCCCCCGAGGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGGCGGCGACGAcaccgtcaccaccgtc                                                <  2:16903828/138‑1 (MQ=255)
                                                                                           cGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGcc                                                                                               >  1:18958163/1‑86 (MQ=255)
                                                                                           cGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTgg                                          >  2:1585087/1‑139 (MQ=255)
                                                                                           cGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTgg                                          >  2:16543839/1‑139 (MQ=255)
                                                                                           cGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTgg                                          >  2:111104/1‑139 (MQ=255)
                                                                                                    tCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                                 <  1:11334388/139‑1 (MQ=255)
                                                                                                    tCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                                 <  1:12113643/139‑1 (MQ=255)
                                                                                                    tCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                                 >  1:14326511/1‑139 (MQ=255)
                                                                                                    tCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                                 >  1:1050475/1‑139 (MQ=255)
                                                                                                    tCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                                 <  1:14709291/139‑1 (MQ=255)
                                                                                                    tCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                                 <  1:3536717/139‑1 (MQ=255)
                                                                                                    tCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                                 <  1:7720166/139‑1 (MQ=255)
                                                                                                    tCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCacc                                 <  1:9816225/139‑1 (MQ=255)
                                                                                                     ccGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCaccg                                <  1:58968/139‑1 (MQ=255)
                                                                                                     ccGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCaccg                                >  1:8392164/1‑139 (MQ=255)
                                                                                                          cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                           <  1:4294578/139‑1 (MQ=255)
                                                                                                          cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                           <  1:14534987/139‑1 (MQ=255)
                                                                                                          cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                           <  1:8875946/139‑1 (MQ=255)
                                                                                                          cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                           <  1:19545432/139‑1 (MQ=255)
                                                                                                          cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                           <  2:5458738/139‑1 (MQ=255)
                                                                                                          cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                           <  1:17590443/139‑1 (MQ=255)
                                                                                                          cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                           <  1:16474198/139‑1 (MQ=255)
                                                                                                          cGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgc                           <  1:16156329/139‑1 (MQ=255)
                                                                                                           gCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgcg                          >  1:3211236/1‑139 (MQ=255)
                                                                                                           gCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACcgcg                          >  2:3031/1‑139 (MQ=255)
                                                                                                                caccacCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAg                     <  1:11916763/139‑1 (MQ=255)
                                                                                                                       aTGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGc              <  2:6242404/139‑1 (MQ=255)
                                                                                                                        tGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCg             >  2:4773003/1‑139 (MQ=255)
                                                                                                                        tGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTACACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCg             >  2:4917304/1‑139 (MQ=255)
                                                                                                                        tGCTCGAACTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCg             <  2:8917167/139‑1 (MQ=255)
                                                                                                                               ccTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTTCGGGCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCt      <  2:7175050/139‑1 (MQ=255)
                                                                                                                               ccTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCt      <  1:12495866/139‑1 (MQ=255)
                                                                                                                               ccTGCTGCCCCCGAAGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCt      <  2:13910339/139‑1 (MQ=255)
                                                                                                                                  gctgcCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGTAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGcc   >  2:5255649/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCG  >  AE015451_phaM1/5659870‑5660139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 445 total aligned reads displayed.
GGTGTCGTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGC  >  AE015451_phaM1/5659853‑5660156
                                                                                                                                                        |                                                                                                                                                       
GGTGTCGTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCC                                                                                                                                                         <  VH01111:79:AACJV3JHV:2:1205:60041:27694/153‑1 (MQ=60)
 GTGTCGTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCC                                                                                                                                                        >  VH01111:79:AACJV3JHV:1:2114:76553:29398/1‑153 (MQ=60)
   GTCGTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCC                                                                                                                                                      <  VH01111:79:AACJV3JHV:2:1301:23022:26047/153‑1 (MQ=60)
     CGTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGA                                                                                                                                                    <  VH01111:79:AACJV3JHV:1:2308:25635:47856/153‑1 (MQ=60)
      GTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGAT                                                                                                                                                   <  VH01111:79:AACJV3JHV:2:1113:50895:21768/153‑1 (MQ=60)
      GTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGAT                                                                                                                                                   <  VH01111:79:AACJV3JHV:1:1212:34516:9481/153‑1 (MQ=60)
      GTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGAT                                                                                                                                                   >  VH01111:79:AACJV3JHV:1:1510:54796:1170/1‑153 (MQ=60)
      GTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGAT                                                                                                                                                   <  VH01111:79:AACJV3JHV:1:1406:19197:23586/153‑1 (MQ=60)
      GTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGAT                                                                                                                                                   >  VH01111:79:AACJV3JHV:2:1614:41920:29455/1‑153 (MQ=60)
         CGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTA                                                                                                                                                <  VH01111:79:AACJV3JHV:2:1308:58924:49939/153‑1 (MQ=60)
           CCATTGGNGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATC                                                                                                                                              >  VH01111:79:AACJV3JHV:1:2303:23060:12416/1‑153 (MQ=60)
           CCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATN                                                                                                                                              >  VH01111:79:AACJV3JHV:2:2413:57617:19818/1‑153 (MQ=60)
           CCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATC                                                                                                                                              <  VH01111:79:AACJV3JHV:1:1213:45158:50829/153‑1 (MQ=60)
           CCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATC                                                                                                                                              >  VH01111:79:AACJV3JHV:2:1602:49551:45035/1‑153 (MQ=60)
           CCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATC                                                                                                                                              >  VH01111:79:AACJV3JHV:1:1502:41030:4635/1‑153 (MQ=60)
           CCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATC                                                                                                                                              >  VH01111:79:AACJV3JHV:2:2101:32395:29511/1‑153 (MQ=60)
           CCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATC                                                                                                                                              <  VH01111:79:AACJV3JHV:1:1214:72141:31083/153‑1 (MQ=60)
               TGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGA                                                                                                                                          <  VH01111:79:AACJV3JHV:2:2505:54815:18872/153‑1 (MQ=60)
                GGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAA                                                                                                                                         >  VH01111:79:AACJV3JHV:1:2411:38833:15956/1‑153 (MQ=60)
                      GCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCT                                                                                                                                   >  VH01111:79:AACJV3JHV:2:1509:66877:7191/1‑153 (MQ=60)
                      GCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCT                                                                                                                                   >  VH01111:79:AACJV3JHV:1:2206:77045:19250/1‑153 (MQ=60)
                      GCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCT                                                                                                                                   >  VH01111:79:AACJV3JHV:2:2109:12020:26785/1‑153 (MQ=60)
                      GCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCT                                                                                                                                   >  VH01111:79:AACJV3JHV:2:2602:56519:21257/1‑153 (MQ=60)
                      GCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCT                                                                                                                                   >  VH01111:79:AACJV3JHV:2:1613:32812:16789/1‑153 (MQ=60)
                           GCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTACTGCCCCCGATGTATCAGGAAACCGCTGCCTA                                                                                                                              >  VH01111:79:AACJV3JHV:1:2208:52580:1265/1‑153 (MQ=60)
                            CCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCCCCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTAC                                                                                                                             <  VH01111:79:AACJV3JHV:1:1403:45726:3802/153‑1 (MQ=60)
                            CCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTAC                                                                                                                             <  VH01111:79:AACJV3JHV:1:2211:36940:37652/153‑1 (MQ=60)
                            CCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTAC                                                                                                                             <  VH01111:79:AACJV3JHV:2:1501:30274:24059/153‑1 (MQ=60)
                            CCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTAC                                                                                                                             <  VH01111:79:AACJV3JHV:2:2309:68202:24078/153‑1 (MQ=60)
                            CCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTAC                                                                                                                             <  VH01111:79:AACJV3JHV:2:2610:29725:34869/153‑1 (MQ=60)
                               ACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:2301:11206:46001/153‑1 (MQ=60)
                               ACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGC                                                                                                                          <  VH01111:79:AACJV3JHV:2:1209:21091:6244/153‑1 (MQ=60)
                               ACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGC                                                                                                                          <  VH01111:79:AACJV3JHV:2:2408:35292:29985/153‑1 (MQ=60)
                               ACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGC                                                                                                                          >  VH01111:79:AACJV3JHV:2:2406:27529:46115/1‑153 (MQ=60)
                               ACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:1108:22965:25839/153‑1 (MQ=60)
                                CCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCC                                                                                                                         >  VH01111:79:AACJV3JHV:2:1407:18193:6907/1‑153 (MQ=60)
                                CCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCC                                                                                                                         <  VH01111:79:AACJV3JHV:2:2204:35898:55278/153‑1 (MQ=60)
                                        TCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGC                                                                                                                 <  VH01111:79:AACJV3JHV:2:2209:50308:56319/153‑1 (MQ=60)
                                        TCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGC                                                                                                                 <  VH01111:79:AACJV3JHV:2:2201:63942:17736/153‑1 (MQ=60)
                                        TCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGC                                                                                                                 <  VH01111:79:AACJV3JHV:1:1505:50403:34453/153‑1 (MQ=60)
                                          GATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCG                                                                                                               >  VH01111:79:AACJV3JHV:2:1101:20447:45887/1‑153 (MQ=60)
                                          GATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCG                                                                                                               >  VH01111:79:AACJV3JHV:2:1312:70020:32673/1‑153 (MQ=60)
                                          GATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCG                                                                                                               >  VH01111:79:AACJV3JHV:2:2210:60779:46493/1‑153 (MQ=60)
                                          GATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCG                                                                                                               >  VH01111:79:AACJV3JHV:2:1302:53432:3215/1‑153 (MQ=60)
                                          GATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCG                                                                                                               >  VH01111:79:AACJV3JHV:1:2311:21204:5941/1‑153 (MQ=60)
                                             CAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGTAAACCGCTGCCTACGGCCACTTCGGCCGTAG                                                                                                            <  VH01111:79:AACJV3JHV:2:2110:67180:50620/153‑1 (MQ=60)
                                             CAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTAG                                                                                                            <  VH01111:79:AACJV3JHV:2:2110:70720:44467/153‑1 (MQ=60)
                                               ACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTC                                                                                                          <  VH01111:79:AACJV3JHV:1:1612:41408:30571/153‑1 (MQ=60)
                                               ACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTC                                                                                                          <  VH01111:79:AACJV3JHV:1:1614:51539:15028/153‑1 (MQ=60)
                                                CACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCC                                                                                                         >  VH01111:79:AACJV3JHV:1:1403:71743:30269/1‑153 (MQ=60)
                                                  CCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGC                                                                                                       <  VH01111:79:AACJV3JHV:1:2104:55591:9728/153‑1 (MQ=60)
                                                  CCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGC                                                                                                       <  VH01111:79:AACJV3JHV:1:2305:43718:47421/153‑1 (MQ=60)
                                                  CCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGC                                                                                                       <  VH01111:79:AACJV3JHV:1:1504:26033:45944/153‑1 (MQ=60)
                                                  CCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGC                                                                                                       <  VH01111:79:AACJV3JHV:1:1407:28608:17206/153‑1 (MQ=60)
                                                  CCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGC                                                                                                       <  VH01111:79:AACJV3JHV:1:1210:42753:48765/153‑1 (MQ=60)
                                                  CCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGC                                                                                                       <  VH01111:79:AACJV3JHV:1:1101:11433:39299/153‑1 (MQ=60)
                                                   CTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCA                                                                                                      >  VH01111:79:AACJV3JHV:2:1408:57257:39829/1‑153 (MQ=60)
                                                   CTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCA                                                                                                      >  VH01111:79:AACJV3JHV:1:2414:39325:56281/1‑153 (MQ=60)
                                                   CTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCA                                                                                                      >  VH01111:79:AACJV3JHV:1:1601:38303:2136/1‑153 (MQ=60)
                                                   CTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCA                                                                                                      >  VH01111:79:AACJV3JHV:1:1313:16054:34377/1‑153 (MQ=60)
                                                     TCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGC                                                                                                    <  VH01111:79:AACJV3JHV:2:1401:62086:16676/153‑1 (MQ=60)
                                                     TCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGC                                                                                                    <  VH01111:79:AACJV3JHV:2:2207:21848:7191/153‑1 (MQ=60)
                                                     TCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGC                                                                                                    <  VH01111:79:AACJV3JHV:1:1213:40272:32086/153‑1 (MQ=60)
                                                     TCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGC                                                                                                    <  VH01111:79:AACJV3JHV:1:1212:18402:42328/153‑1 (MQ=60)
                                                       GGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAG                                                                                                  <  VH01111:79:AACJV3JHV:1:2502:25503:37841/153‑1 (MQ=60)
                                                       GGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAG                                                                                                  <  VH01111:79:AACJV3JHV:1:1501:69281:38901/153‑1 (MQ=60)
                                                        GTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGA                                                                                                 >  VH01111:79:AACJV3JHV:2:1301:59643:13968/1‑153 (MQ=60)
                                                        GTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCACGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGA                                                                                                 >  VH01111:79:AACJV3JHV:1:1102:12058:18720/1‑153 (MQ=60)
                                                          ACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAG                                                                                               <  VH01111:79:AACJV3JHV:2:2214:44173:30988/153‑1 (MQ=60)
                                                          ACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAG                                                                                               <  VH01111:79:AACJV3JHV:1:1608:32793:33695/153‑1 (MQ=60)
                                                           CCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGA                                                                                              >  VH01111:79:AACJV3JHV:2:2404:45536:13230/1‑153 (MQ=60)
                                                            CGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCCGCAGAAGAC                                                                                             <  VH01111:79:AACJV3JHV:2:2514:55761:52949/153‑1 (MQ=60)
                                                            CGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGAC                                                                                             <  VH01111:79:AACJV3JHV:1:2503:65797:19440/153‑1 (MQ=60)
                                                            CGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGAC                                                                                             <  VH01111:79:AACJV3JHV:2:2106:34610:32067/153‑1 (MQ=60)
                                                            CGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGAC                                                                                             <  VH01111:79:AACJV3JHV:1:1613:23420:32540/153‑1 (MQ=60)
                                                            CGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGAC                                                                                             <  VH01111:79:AACJV3JHV:1:1513:15618:12511/153‑1 (MQ=60)
                                                            CGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGAC                                                                                             <  VH01111:79:AACJV3JHV:1:2402:25938:42025/153‑1 (MQ=60)
                                                            CGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGAC                                                                                             <  VH01111:79:AACJV3JHV:2:1401:40746:26804/153‑1 (MQ=60)
                                                            CGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGAC                                                                                             <  VH01111:79:AACJV3JHV:2:1106:23931:32673/153‑1 (MQ=60)
                                                            CGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGNGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGAC                                                                                             <  VH01111:79:AACJV3JHV:2:1202:73523:15502/153‑1 (MQ=60)
                                                             GGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACT                                                                                            >  VH01111:79:AACJV3JHV:2:2102:63014:15899/1‑153 (MQ=60)
                                                                  GGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGG                                                                                       >  VH01111:79:AACJV3JHV:2:1604:10165:44846/1‑153 (MQ=60)
                                                                  GGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGG                                                                                       >  VH01111:79:AACJV3JHV:1:1603:51842:27486/1‑153 (MQ=60)
                                                                   GTCTCCGACGACAAGATCATCCNGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGC                                                                                      >  VH01111:79:AACJV3JHV:2:2213:36504:53271/1‑153 (MQ=60)
                                                                   GTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCTCCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTACGGCCGTACTCCGCAGCAGAAGTCTGTCGGC                                                                                      >  VH01111:79:AACJV3JHV:2:2113:55875:28678/1‑153 (MQ=60)
                                                                   GTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGC                                                                                      >  VH01111:79:AACJV3JHV:1:2610:71099:7285/1‑153 (MQ=60)
                                                                   GTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGC                                                                                      >  VH01111:79:AACJV3JHV:1:2501:67520:23358/1‑153 (MQ=60)
                                                                   GTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGC                                                                                      >  VH01111:79:AACJV3JHV:1:2313:62730:1757/1‑153 (MQ=60)
                                                                    ccagcggcaactacgtgttctacggcGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCG                                                                                     >  VH01111:79:AACJV3JHV:2:1403:58867:17243/27‑153 (MQ=60)
                                                                           CGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACAC                                                                              >  VH01111:79:AACJV3JHV:1:1304:38246:17565/1‑153 (MQ=60)
                                                                           CGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACAC                                                                              >  VH01111:79:AACJV3JHV:2:2101:51331:35797/1‑153 (MQ=60)
                                                                            GACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACC                                                                             <  VH01111:79:AACJV3JHV:2:2606:21905:43483/153‑1 (MQ=60)
                                                                             ACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCT                                                                            <  VH01111:79:AACJV3JHV:1:1611:44684:32067/153‑1 (MQ=60)
                                                                             ACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCT                                                                            <  VH01111:79:AACJV3JHV:2:2106:70020:26577/153‑1 (MQ=60)
                                                                             ACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCT                                                                            <  VH01111:79:AACJV3JHV:1:1105:62446:39526/153‑1 (MQ=60)
                                                                             ACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCT                                                                            <  VH01111:79:AACJV3JHV:1:2205:73012:11734/153‑1 (MQ=60)
                                                                             ACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCT                                                                            <  VH01111:79:AACJV3JHV:2:2603:52997:48216/153‑1 (MQ=60)
                                                                             ACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCT                                                                            <  VH01111:79:AACJV3JHV:1:1208:10203:27391/153‑1 (MQ=60)
                                                                             ACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCT                                                                            <  VH01111:79:AACJV3JHV:2:1612:8555:38731/153‑1 (MQ=60)
                                                                                 GATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCAC                                                                        <  VH01111:79:AACJV3JHV:1:2603:28419:49958/153‑1 (MQ=60)
                                                                                 GATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCAC                                                                        >  VH01111:79:AACJV3JHV:1:1608:42355:48670/1‑153 (MQ=60)
                                                                                 GATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCAC                                                                        >  VH01111:79:AACJV3JHV:2:1303:43832:45944/1‑153 (MQ=60)
                                                                                 GATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCAC                                                                        >  VH01111:79:AACJV3JHV:2:2303:56954:40776/1‑153 (MQ=60)
                                                                                 GATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCAC                                                                        >  VH01111:79:AACJV3JHV:2:1514:40026:34225/1‑153 (MQ=60)
                                                                                  ATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACC                                                                       <  VH01111:79:AACJV3JHV:1:2212:14331:19099/153‑1 (MQ=60)
                                                                                  ATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACC                                                                       <  VH01111:79:AACJV3JHV:1:1114:19670:4067/153‑1 (MQ=60)
                                                                                   TCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCA                                                                      >  VH01111:79:AACJV3JHV:1:2212:65532:40189/1‑153 (MQ=60)
                                                                                   TCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCA                                                                      >  VH01111:79:AACJV3JHV:1:1309:73731:27561/1‑153 (MQ=60)
                                                                                   TCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCA                                                                      <  VH01111:79:AACJV3JHV:2:2509:52126:50109/153‑1 (MQ=60)
                                                                                   TCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCA                                                                      >  VH01111:79:AACJV3JHV:1:2407:49683:36838/1‑153 (MQ=60)
                                                                                    CATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCAC                                                                     <  VH01111:79:AACJV3JHV:1:2511:9332:17206/153‑1 (MQ=60)
                                                                                    CATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCAC                                                                     <  VH01111:79:AACJV3JHV:1:2111:45176:20140/153‑1 (MQ=60)
                                                                                    CATCCAGCAGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCAGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCAC                                                                     <  VH01111:79:AACJV3JHV:1:2101:49683:3139/153‑1 (MQ=60)
                                                                                      TCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCT                                                                   >  VH01111:79:AACJV3JHV:2:1411:62919:35910/1‑153 (MQ=60)
                                                                                      TCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCT                                                                   >  VH01111:79:AACJV3JHV:1:2610:50441:6055/1‑153 (MQ=60)
                                                                                           CTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACC                                                              <  VH01111:79:AACJV3JHV:2:2505:28816:45774/153‑1 (MQ=60)
                                                                                           CTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACC                                                              <  VH01111:79:AACJV3JHV:2:1511:52675:42025/153‑1 (MQ=60)
                                                                                           CTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACC                                                              <  VH01111:79:AACJV3JHV:1:1205:75530:27050/153‑1 (MQ=60)
                                                                                           CTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACACCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACC                                                              <  VH01111:79:AACJV3JHV:2:1511:55004:42423/153‑1 (MQ=60)
                                                                                            TGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCT                                                             <  VH01111:79:AACJV3JHV:1:2213:71459:14347/153‑1 (MQ=60)
                                                                                            TGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCT                                                             <  VH01111:79:AACJV3JHV:1:1301:72747:47818/153‑1 (MQ=60)
                                                                                            TGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCT                                                             <  VH01111:79:AACJV3JHV:1:2411:20504:49844/153‑1 (MQ=60)
                                                                                              GTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGctgtctcttatacacatctccgagcccacgagacattgg                                                           >  VH01111:79:AACJV3JHV:2:2513:7949:27448/1‑114 (MQ=60)
                                                                                              GTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACctgtctcttatacacatctccgagcccacgagac                                                           >  VH01111:79:AACJV3JHV:2:1103:23912:42536/1‑119 (MQ=60)
                                                                                               TGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTNACCTGGG                                                          <  VH01111:79:AACJV3JHV:1:2403:38454:9481/153‑1 (MQ=60)
                                                                                               TGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGG                                                          <  VH01111:79:AACJV3JHV:2:1609:52429:54350/153‑1 (MQ=60)
                                                                                               TGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGG                                                          <  VH01111:79:AACJV3JHV:2:1108:44608:38352/153‑1 (MQ=60)
                                                                                               TGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGG                                                          <  VH01111:79:AACJV3JHV:1:1207:11831:16145/153‑1 (MQ=60)
                                                                                               TGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGG                                                          <  VH01111:79:AACJV3JHV:1:1205:63923:10220/153‑1 (MQ=60)
                                                                                                      GTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCAC                                                   <  VH01111:79:AACJV3JHV:1:1411:59984:35588/153‑1 (MQ=60)
                                                                                                      GTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCAC                                                   <  VH01111:79:AACJV3JHV:2:1507:33645:46664/153‑1 (MQ=60)
                                                                                                      GTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCAC                                                   <  VH01111:79:AACJV3JHV:1:2302:61518:6490/153‑1 (MQ=60)
                                                                                                      GTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCAC                                                   <  VH01111:79:AACJV3JHV:1:1604:58488:13230/153‑1 (MQ=60)
                                                                                                      GTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCAC                                                   <  VH01111:79:AACJV3JHV:1:2203:65911:47004/153‑1 (MQ=60)
                                                                                                       TGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACC                                                  >  VH01111:79:AACJV3JHV:2:1603:21696:51908/1‑153 (MQ=60)
                                                                                                       TGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACC                                                  >  VH01111:79:AACJV3JHV:1:1204:71364:24627/1‑153 (MQ=60)
                                                                                                        GCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCG                                                 >  VH01111:79:AACJV3JHV:2:2114:34554:42801/1‑153 (MQ=60)
                                                                                                        GCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCG                                                 >  VH01111:79:AACJV3JHV:1:2412:53110:48557/1‑153 (MQ=60)
                                                                                                        GCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTCGGAGCGCACCG                                                 >  VH01111:79:AACJV3JHV:2:1112:9199:41117/1‑153 (MQ=60)
                                                                                                              ACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCG                                           >  VH01111:79:AACJV3JHV:1:1412:62976:12946/1‑153 (MQ=60)
                                                                                                                 TGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCC                                        <  VH01111:79:AACJV3JHV:2:2514:62843:19705/153‑1 (MQ=60)
                                                                                                                 TGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCC                                        <  VH01111:79:AACJV3JHV:2:2502:42374:39564/153‑1 (MQ=60)
                                                                                                                     TCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTC                                    <  VH01111:79:AACJV3JHV:2:1608:33910:39242/153‑1 (MQ=60)
                                                                                                                     TCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTC                                    <  VH01111:79:AACJV3JHV:2:1308:21583:35588/153‑1 (MQ=60)
                                                                                                                     TCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTC                                    <  VH01111:79:AACJV3JHV:2:1610:9691:18436/153‑1 (MQ=60)
                                                                                                                     TCCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAACGCACCGACCGCGCCCAGTC                                    <  VH01111:79:AACJV3JHV:1:1104:24631:29625/153‑1 (MQ=60)
                                                                                                                      CCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCG                                   <  VH01111:79:AACJV3JHV:2:2410:49153:40132/153‑1 (MQ=60)
                                                                                                                      CCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCG                                   <  VH01111:79:AACJV3JHV:2:2410:48793:40302/153‑1 (MQ=60)
                                                                                                                      CCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCG                                   <  VH01111:79:AACJV3JHV:2:2410:53262:42347/153‑1 (MQ=60)
                                                                                                                      CCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCG                                   <  VH01111:79:AACJV3JHV:2:2604:64263:22374/153‑1 (MQ=60)
                                                                                                                      CCGTACGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACAGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCG                                   <  VH01111:79:AACJV3JHV:2:2410:49020:40302/153‑1 (MQ=60)
                                                                                                                           CGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAGACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCG                              <  VH01111:79:AACJV3JHV:2:2404:51103:15047/153‑1 (MQ=60)
                                                                                                                           CGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCG                              <  VH01111:79:AACJV3JHV:1:2510:33096:19193/153‑1 (MQ=60)
                                                                                                                           CGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCG                              <  VH01111:79:AACJV3JHV:1:2513:30975:38012/153‑1 (MQ=60)
                                                                                                                           CGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCG                              <  VH01111:79:AACJV3JHV:2:2212:58261:32540/153‑1 (MQ=60)
                                                                                                                           CGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCG                              <  VH01111:79:AACJV3JHV:2:1410:52107:3707/153‑1 (MQ=60)
                                                                                                                           CGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCG                              <  VH01111:79:AACJV3JHV:2:1207:58204:17376/153‑1 (MQ=60)
                                                                                                                           CGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCG                              <  VH01111:79:AACJV3JHV:2:1607:39685:23245/153‑1 (MQ=60)
                                                                                                                           CGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCG                              <  VH01111:79:AACJV3JHV:2:1309:45517:32446/153‑1 (MQ=60)
                                                                                                                           CGCCATCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCG                              <  VH01111:79:AACJV3JHV:1:1602:15088:11034/153‑1 (MQ=60)
                                                                                                                                TCACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACG                         >  VH01111:79:AACJV3JHV:2:1604:59321:14858/1‑153 (MQ=60)
                                                                                                                                 CACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGGCTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGC                        <  VH01111:79:AACJV3JHV:1:2613:18780:18777/153‑1 (MQ=60)
                                                                                                                                 CACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGC                        <  VH01111:79:AACJV3JHV:2:1305:13706:23510/153‑1 (MQ=60)
                                                                                                                                 CACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGC                        <  VH01111:79:AACJV3JHV:2:1211:15524:33241/153‑1 (MQ=60)
                                                                                                                                 CACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGC                        <  VH01111:79:AACJV3JHV:2:2207:55458:40378/153‑1 (MQ=60)
                                                                                                                                 CACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGC                        <  VH01111:79:AACJV3JHV:2:1207:46256:32805/153‑1 (MQ=60)
                                                                                                                                 CACCACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCACACGTTGCGTGACGC                        >  VH01111:79:AACJV3JHV:2:1308:42166:42347/1‑153 (MQ=60)
                                                                                                                                     ACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGNGCGCANCGACCGCGCCCAGTCGTTGCGTGACGCTGCC                    >  VH01111:79:AACJV3JHV:2:2305:64604:17982/1‑153 (MQ=60)
                                                                                                                                     ACCATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCC                    >  VH01111:79:AACJV3JHV:1:2304:30312:34661/1‑153 (MQ=60)
                                                                                                                                        ATGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGC                 >  VH01111:79:AACJV3JHV:1:1411:38549:6358/1‑153 (MQ=60)
                                                                                                                                         TGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCC                >  VH01111:79:AACJV3JHV:1:2401:21394:23131/1‑153 (MQ=60)
                                                                                                                                         TGCTCGACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCC                >  VH01111:79:AACJV3JHV:2:1201:37224:28394/1‑153 (MQ=60)
                                                                                                                                              GACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAA           <  VH01111:79:AACJV3JHV:1:2104:12948:29606/153‑1 (MQ=60)
                                                                                                                                              GACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAA           <  VH01111:79:AACJV3JHV:1:1612:17152:16278/153‑1 (MQ=60)
                                                                                                                                              GACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGACCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAA           <  VH01111:79:AACJV3JHV:1:1402:63752:27732/153‑1 (MQ=60)
                                                                                                                                               ACCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAG          >  VH01111:79:AACJV3JHV:1:1506:64718:50696/1‑153 (MQ=60)
                                                                                                                                                CCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGC         <  VH01111:79:AACJV3JHV:2:1307:25256:29303/153‑1 (MQ=60)
                                                                                                                                                CCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGC         <  VH01111:79:AACJV3JHV:1:1609:33929:43275/153‑1 (MQ=60)
                                                                                                                                                CCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGC         <  VH01111:79:AACJV3JHV:1:2106:60514:22298/153‑1 (MQ=60)
                                                                                                                                                CCTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGC         <  VH01111:79:AACJV3JHV:2:1304:12835:41684/153‑1 (MQ=60)
                                                                                                                                                 CTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCT        <  VH01111:79:AACJV3JHV:2:2208:6510:27561/153‑1 (MQ=60)
                                                                                                                                                 CTGCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCT        <  VH01111:79:AACJV3JHV:1:2602:18932:31310/153‑1 (MQ=60)
                                                                                                                                                   GCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTC      >  VH01111:79:AACJV3JHV:1:2511:54114:30136/1‑153 (MQ=60)
                                                                                                                                                   GCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTC      >  VH01111:79:AACJV3JHV:2:2407:49570:47478/1‑153 (MQ=60)
                                                                                                                                                   GCTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTC      >  VH01111:79:AACJV3JHV:1:2611:68675:28224/1‑153 (MQ=60)
                                                                                                                                                    CTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCC     <  VH01111:79:AACJV3JHV:2:2503:35311:30458/153‑1 (MQ=60)
                                                                                                                                                    CTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCC     <  VH01111:79:AACJV3JHV:1:2209:50535:22734/153‑1 (MQ=60)
                                                                                                                                                    CTGCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCC     <  VH01111:79:AACJV3JHV:2:1306:62616:52192/153‑1 (MQ=60)
                                                                                                                                                      GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCTCTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTG   >  VH01111:79:AACJV3JHV:2:2414:44078:28356/1‑153 (MQ=60)
                                                                                                                                                      GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTgggggggggggggcgggcgggggggggaggg   >  VH01111:79:AACJV3JHV:1:1514:22340:30060/1‑122 (MQ=60)
                                                                                                                                                      GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTG   >  VH01111:79:AACJV3JHV:1:2506:60590:52248/1‑153 (MQ=60)
                                                                                                                                                      GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTG   >  VH01111:79:AACJV3JHV:2:2414:43169:28356/1‑153 (MQ=60)
                                                                                                                                                      GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTG   >  VH01111:79:AACJV3JHV:1:1408:35747:45584/1‑153 (MQ=60)
                                                                                                                                                      GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTG   >  VH01111:79:AACJV3JHV:2:2312:19879:30855/1‑153 (MQ=60)
                                                                                                                                                      GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTG   >  VH01111:79:AACJV3JHV:1:1601:62086:31859/1‑153 (MQ=60)
                                                                                                                                                      GCCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTC   >  VH01111:79:AACJV3JHV:1:1114:68069:23983/1‑153 (MQ=60)
                                                                                                                                                      GCCCCCGATGTATCAGGAAACCGCT‑CCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGC  >  VH01111:79:AACJV3JHV:2:2210:73125:32143/1‑153 (MQ=60)
                                                                                                                                                       CCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGC  <  VH01111:79:AACJV3JHV:1:2401:12286:17774/153‑1 (MQ=60)
                                                                                                                                                       CCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGC  <  VH01111:79:AACJV3JHV:1:1306:16054:27978/153‑1 (MQ=60)
                                                                                                                                                       CCCCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGC  <  VH01111:79:AACJV3JHV:2:2608:52391:40681/153‑1 (MQ=60)
                                                                                                                                                        |                                                                                                                                                       
GGTGTCGTACGCCATTGGCGTGGCCCAGCCGACCTCCATCTCGATCAACACCTTCGGTACCGGCAAGGTCTCCGACGACAAGATCATCCAGCTGGTGCGCGAGTGCTTCGACCTGCGTCCGTACGCCATCACCACCATGCTCGACCTGCTGCACCCGATGTATCAGGAAACCGCTGCCTACGGCCACTTCGGCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGC  >  AE015451_phaM1/5659853‑5660156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑