Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F6 I1 R1	81	160.8	7353682	98.1%	7213962	137.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	4,740,819	2 bp→CT	intergenic (+56/+696)	gltA CDS (citrate synthase) → / ← yeiW CDS (putative oxidoreductase)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,740,819	0	T	C	96.0%	343.7 / NA	103	intergenic (+56/+697)	gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)	–/–
Reads supporting (aligned to +/- strand): ref base T (1/2); major base C (57/39); minor base G (0/4); total (58/45)
Fisher's exact test for biased strand distribution p-value = 3.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AE015451_phaM1	4,740,820	0	G	T	95.1%	348.4 / 0.2	103	intergenic (+57/+696)	gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)	–/–
Reads supporting (aligned to +/- strand): ref base G (1/4); new base T (56/41); total (58/45)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGC  >  AE015451_phaM1/4740686‑4740958

||

tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                                        <  2:750782/139‑1 (MQ=255)

tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                                        <  2:752373/139‑1 (MQ=255)

tACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                                        <  2:1655621/139‑1 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGcg                                                                                                                                   >  2:1435963/1‑138 (MQ=255)

        cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGGT‑CC‑GGcccagt                                                                                                                               >  2:2319228/1‑135 (MQ=255)

        cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑CGCCTCtt                                                                                                                               >  2:2226982/1‑139 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCNACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCgg                                                                                                                          <  1:2012394/139‑1 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTGGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGGCTCTTCGCgg                                                                                                                          >  2:3308631/1‑139 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGGCTCTTCGCgg                                                                                                                          >  1:3107280/1‑139 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCgg                                                                                                                          <  1:2858505/139‑1 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCgg                                                                                                                          <  1:1120332/139‑1 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCgg                                                                                                                          <  1:1010683/139‑1 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCgg                                                                                                                          <  1:503861/139‑1 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCgg                                                                                                                          <  1:473616/139‑1 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCgg                                                                                                                          <  1:462652/139‑1 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCgg                                                                                                                          >  1:1811382/1‑139 (MQ=255)

              tCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGGCGCGTggcggc                                                                                                                         >  1:3636267/1‑139 (MQ=255)

              tCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGc                                                                                                                         >  1:1034240/1‑139 (MQ=255)

                cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCt                                                                                                                       <  1:2662251/139‑1 (MQ=255)

                cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCt                                                                                                                       <  1:1680505/139‑1 (MQ=255)

                       gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCcgc                                                                                                                <  2:1034240/139‑1 (MQ=255)

                                cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCG‑AAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCg                                                                                                      >  2:163145/1‑139 (MQ=255)

                                     aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcac                                                                                                  >  2:2226815/1‑139 (MQ=39)

                                      gCATAAGCGCGAATTGTTTGCCGTTAAATACCGAATTTAAGATGCTTTAGCCTAACGCAATACGGCTGCCCTCGGGCTGCGTTTTTTTTTTGTCCTGG‑GCC‑GGCCTCTGCGGGGCCTTGCCCGCGAAGAGGCCGGcaca                                                                                                 <  1:2482267/139‑1 (MQ=255)

                                      gCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTTGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGCcaca                                                                                                 >  2:267782/1‑139 (MQ=255)

                                      gCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcaca                                                                                                 >  2:2056152/1‑139 (MQ=39)

                                      gCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGcaca                                                                                                 >  2:237566/1‑139 (MQ=39)

                                        aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑NGCCTCTTCGCGGCCTTGCCCGCGAAGAGGGCGGCACAGa                                                                                               >  2:3374911/1‑139 (MQ=255)

                                        aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGGCTTGCCCGCGAAGAGGGCGGCAgaga                                                                                               >  2:2465072/1‑139 (MQ=38)

                                        aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGa                                                                                               >  2:355253/1‑139 (MQ=39)

                                        aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGa                                                                                               >  2:647031/1‑139 (MQ=39)

                                             cgcgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAcacca                                                                                          <  1:1347306/139‑1 (MQ=39)

                                             cgcgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCCGTGCC‑GGGCTCTTGGCGGCCGTGGCCCGGTAGTGGGCGGCCCCGAcacca                                                                                          >  1:2960090/1‑139 (MQ=255)

                                             cgccACATGGTTGCCGTTAAAGACCCATATTAAGCTCCAGTCGCCGAACCCATGAAGGCTGCCCGCGGCCAGCCTTTTTTT‑TTTGACCTGTCCC‑GGCCTCTTCGGGGCCTGGCCCGCTAAGAGGCCGGCACAGAcacca                                                                                          <  1:1857124/139‑1 (MQ=255)

                                               cgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAACAGGCCGGCACAGACACcaca                                                                                        >  1:642475/1‑139 (MQ=255)

                                               cgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCGCTTCGCCGCCCTGCCCCCCCAGACGCCCGCCCACACACcaca                                                                                        >  1:2970803/1‑139 (MQ=255)

                                                gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGGCTTGCCCGCGAACAGGCCGGCACAGAGACCACaa                                                                                       >  2:3137253/1‑139 (MQ=38)

                                                  cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTGCTGTGCC‑GGCCTCTTCGCGGCCTTGCCGGCGAACACGCCGCGACAGACACCACaaca                                                                                     >  2:41903/1‑137 (MQ=255)

                                                  cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaac                                                                                     >  2:1666103/1‑138 (MQ=39)

                                                  cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                                     >  2:2939811/1‑139 (MQ=39)

                                                  cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                                     >  2:1337033/1‑139 (MQ=39)

                                                  cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                                     >  2:489237/1‑139 (MQ=39)

                                                  cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                                     >  2:488515/1‑139 (MQ=39)

                                                  cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                                     >  2:3390029/1‑139 (MQ=39)

                                                  cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaaaa                                                                                     >  2:3288007/1‑139 (MQ=39)

                                                  cATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGGGCC‑GGCCACTACGCGGCCTTGCCCGCCAAGAGGCCGGCACAGACACCACaaaa                                                                                     <  2:951605/139‑1 (MQ=255)

                                                   aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                                    <  1:176401/139‑1 (MQ=39)

                                                   aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                                    <  1:1184796/139‑1 (MQ=39)

                                                   aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTGGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                                    <  1:709007/139‑1 (MQ=255)

                                                   aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCAGTTCGGGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                                    <  1:1110349/139‑1 (MQ=255)

                                                       ttGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTGGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCa                                                                                >  2:1558280/1‑139 (MQ=255)

                                                       ttGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCCCGGCCTTGCCCGCGAAGAGGCCCGGACAGACACCACAACACCTCa                                                                                >  2:3586599/1‑139 (MQ=255)

                                                       ttGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCACTTGCCGGCCATTGCCGCGAAATTGGCGGGACATACACCACAAAACcaca                                                                                >  2:2756038/1‑139 (MQ=255)

                                                          cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGGCTCTTCGCGGCCCTTCCCGCGACCCGGCGGCCCCAGGCACCCCACACCCTCACtt                                                                             >  1:3465586/1‑139 (MQ=255)

                                                          cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACCGTCACCACAAAACCTCAGtt                                                                             >  1:2185171/1‑139 (MQ=39)

                                                                       cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccgc                                                                >  1:1228010/1‑139 (MQ=39)

                                                                            aaGCTGCAGTAGCCGAACGCATGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                           <  1:514823/139‑1 (MQ=39)

                                                                            aaGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTT‑TTTGACCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                           <  1:2372311/139‑1 (MQ=39)

                                                                             aGCTGCAGTAGCCAAACAAATTAGGGCGCCCCGCGCGCAG‑GTTTTTTT‑TTTTGGGGGGGCCGGGCCTGTGCGGGGCCTGCCCCGCCAAGCGCCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc                                                          <  1:3669114/139‑1 (MQ=255)

                                                                             aGCTGCAGACGCCGAACGAAGGAGGGGGGCCCGGGGGCAGCGTTTTTTT‑TTTGCCGTGGGCC‑GGCCTGTCGGCGGCGGGCCCCCCCAAGCGCCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc                                                          <  1:2297122/139‑1 (MQ=255)

                                                                                  cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CGTTTTTTATTTGTCGTGTGCC‑GGCCACTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                     <  2:2868476/139‑1 (MQ=255)

                                                                                    gtgcccGAACCCAAGAAGGCTGCCCTCGGGGAG‑CGTTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCCCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:362163/135‑1 (MQ=255)

                                                                                    ggaGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  2:1353796/137‑1 (MQ=39)

                                                                                    gaaGCCGAACGCAAGAAGGCTCCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGGGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:327088/137‑1 (MQ=38)

                                                                                    gaaGCCGAACGCAAGAAGCCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCACTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  2:2387026/137‑1 (MQ=255)

                                                                                    gTAGCCGAACGCAAGACGGCTGCCCGCGGGCAGCCTTTTTTT‑TTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTGGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:176881/139‑1 (MQ=255)

                                                                                    gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTGCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  2:3602506/139‑1 (MQ=39)

                                                                                    gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:1014398/139‑1 (MQ=39)

                                                                                    gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  2:1238604/139‑1 (MQ=39)

                                                                                    gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTACGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  2:1571499/139‑1 (MQ=255)

                                                                                    gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGGCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  2:2210648/139‑1 (MQ=39)

                                                                                    gTAGCCCAACGCAAGAAGGCTGCCCTCGGGCAGCGTTTTTTTTTTGTCCTGG‑GCC‑GGCGTCTTCGGGGCGTGGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:3260058/139‑1 (MQ=255)

                                                                                    gTACCCGAACGCAAGAAGGCTGCCCGCGGGCAGCGTTTTTTT‑TTTGACGTGTGCG‑GGCCTGTTCGCGGCGTGGCCGGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:3260123/139‑1 (MQ=255)

                                                                                    ctccccGAACCCAAGAAGGCTGCCCGCGGGCAGCGTTTTTTT‑TTGTTCCGGGGCG‑GGCGAGTTCGCGGCGTCGCCCGCGAAGAGCCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:2866391/135‑1 (MQ=255)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGGCTCATCGCGGGCCTGGCCGCGGCGAGGGCGGGACTGTTCACAAAAAAACTCCTTTTTTATCCCCCCGCTCACCCACCCCttt                                                  >  1:2726792/1‑139 (MQ=255)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGTGGCCGGCCCAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCttt                                                  >  1:1389953/1‑139 (MQ=255)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCttt                                                  >  1:1084694/1‑139 (MQ=255)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCGttt                                                  >  1:262487/1‑139 (MQ=39)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCttt                                                  >  1:315195/1‑139 (MQ=39)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACCCAGCCCgtt                                                  >  1:2320461/1‑139 (MQ=39)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTATCCGCCCGCTCACGCAGCCCttt                                                  >  2:1312165/1‑139 (MQ=255)

                                                                                            aCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGGCGCGTCGGGGGCGTGCCCGCGCCGAGGGCGGCCCCCCCAACACAAAACCTCAGATTTTATACGCCCCCCCACCCACCCCCTTCTGGGtg                                           >  1:2998124/1‑138 (MQ=255)

                                                                                              gCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTGTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                                         <  2:1312254/139‑1 (MQ=255)

                                                                                               cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCATCCCGGCCATGGCCCCCAACACGCCCGCACACACACCAAAAAACCCAACTTTTTATACGCCCGCTGACGCCGCCCTTTGACGGttttt                                        >  1:1609102/1‑138 (MQ=255)

                                                                                                aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGa                                       >  2:1662146/1‑139 (MQ=39)

                                                                                                     ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGGCTCTTCGCGGCCTTGCCCGCGTAGAGGCCGGCCCACACACCACAAAACCTCTGTTTTTGTCCGCCCCCTCACCCCGCCCTTTCTAGGTAATGTACCgc                                  >  1:3576300/1‑139 (MQ=255)

                                                                                                     ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                                  >  2:713520/1‑139 (MQ=39)

                                                                                                     ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                                  >  2:2723232/1‑139 (MQ=39)

                                                                                                     ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACCCAGCCCTTTGCGGGTATTGATCGgc                                  >  2:2723246/1‑139 (MQ=38)

                                                                                                      gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcg                                 <  2:315195/139‑1 (MQ=39)

                                                                                                          cccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                             <  1:656874/139‑1 (MQ=39)

                                                                                                             tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCcaccac                          >  1:1368270/1‑139 (MQ=255)

                                                                                                             tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACACCACAAAACCTCAGTTTTTATCCGCCCGCTCACGCAGCCCGTTCAGGGGATTGAACGGGGCATccac                          >  1:491671/1‑139 (MQ=38)

                                                                                                             tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCC‑GGCCTCTTAGCGGCCTTGGCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCACTTTTTTTACCCCCGCTCACGCAGCCCATTAACGGTTATGAACGGCGCATccac                          >  1:973244/1‑139 (MQ=255)

                                                                                                                        ttttttATTTGTCCTGTGCC‑GGCCTCTTCGGGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCACTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACCGCGCATCCACCTCGAACttg                >  2:394636/1‑139 (MQ=255)

                                                                                                                        ttttttATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAACCCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttg                >  2:840368/1‑139 (MQ=255)

                                                                                                                        ttttttATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTATCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttg                >  2:393893/1‑139 (MQ=255)

                                                                                                                         tttttATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGcc                                                      >  2:1664083/1‑100 (MQ=255)

                                                                                                                         tttttATTTGTCCTGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGcc                                                      <  1:1664083/100‑1 (MQ=255)

                                                                                                                                      ggTGCC‑GGCGTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGc  <  1:958337/139‑1 (MQ=255)

||

TACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCC‑GGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGC  >  AE015451_phaM1/4740686‑4740958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A