Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F6 I2 R1	82	424.1	19537984	98.2%	19186300	137.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	4,740,819	2 bp→CT	intergenic (+56/+696)	gltA CDS (citrate synthase) → / ← yeiW CDS (putative oxidoreductase)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	4,740,819	0	T	C	96.2%	896.7 / NA	268	intergenic (+56/+697)	gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)	–/–
Reads supporting (aligned to +/- strand): ref base T (2/1); major base C (145/108); minor base G (0/10); total (147/121)
Fisher's exact test for biased strand distribution p-value = 2.66e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AE015451_phaM1	4,740,820	0	G	T	98.1%	962.0 / ‑0.7	268	intergenic (+57/+696)	gltA CDS (citrate synthase)/yeiW CDS (putative oxidoreductase)	–/–
Reads supporting (aligned to +/- strand): ref base G (1/4); new base T (145/117); total (147/121)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

TACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGC  >  AE015451_phaM1/4740686‑4740958

||

gaCAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                                        <  2:2349686/138‑1 (MQ=255)

 aCAAGAGCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                       <  2:796991/139‑1 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATTTGTCCTGGTGCCggg                                                                                                                                     >  1:4133928/1‑138 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgggg                                                                                                                                    >  1:9289617/1‑137 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgggc                                                                                                                                    >  1:6837372/1‑137 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGcc                                                                                                                                    >  1:4935381/1‑139 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGcc                                                                                                                                    >  1:9599757/1‑139 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGcc                                                                                                                                    >  1:9238888/1‑139 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGcc                                                                                                                                    >  1:4566321/1‑139 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGcc                                                                                                                                    >  1:8866550/1‑139 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGcc                                                                                                                                    >  1:3611501/1‑139 (MQ=255)

    aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGcc                                                                                                                                    >  1:3525821/1‑139 (MQ=255)

      aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGccgc                                                                                                                                  >  1:7102662/1‑137 (MQ=255)

       tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                                 <  1:3255957/139‑1 (MQ=255)

       tCGGCCGTCCGCGCCAGCTGTACACCGGCGACCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                                 <  2:5466025/139‑1 (MQ=255)

        cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCtt                                                                                                                                <  2:1566186/139‑1 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                           >  2:5170625/1‑139 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                           >  2:57799/1‑139 (MQ=255)

             gTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTCTTCGCgg                                                                                                                           >  2:6751731/1‑139 (MQ=255)

                cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCt                                                                                                                        >  1:3162729/1‑139 (MQ=255)

                  cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTg                                                                                                                      >  2:262342/1‑139 (MQ=255)

                   gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                                     >  2:9113818/1‑139 (MQ=255)

                   gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                                     >  2:9675030/1‑139 (MQ=255)

                   gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                                     >  2:356356/1‑139 (MQ=255)

                   gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTCTTCGCGGCCTTGc                                                                                                                     >  2:5423845/1‑139 (MQ=255)

                       gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                                 <  2:3476008/139‑1 (MQ=255)

                            acacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACACAAGAAGGCTGCCCTCGGGCAGCGTTTTTTT‑TTTGTCGTGGGCGGGCCTCTTCGCGGCCTTGCCCGCGAag‑a                                                                                                           <  2:8439227/139‑1 (MQ=255)

                             cacCGGCGAGCAGAAGCGCGACATCTTTGCCCTGAAAGACCCCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCACCCTTTTTTT‑TTTGTCATGTGCCGGCCTCTTCGCGGCGTTGCCCGCGAag‑ag                                                                                                          <  2:7297645/139‑1 (MQ=255)

                                   cGAGCATAAGCGCGACATGGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTT‑TTTGTCCTGTGCGGGCCTCTGCGCGGCCTGGCCCGCGAAG‑AGGCCGGc                                                                                                    <  1:6273240/139‑1 (MQ=255)

                                   cGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGc                                                                                                    >  1:1060980/1‑139 (MQ=39)

                                     aGCAGAAGCGCGACATCGTTGCCGTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGcac                                                                                                  <  2:6212127/139‑1 (MQ=255)

                                     aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAG‑ATGCCGGcac                                                                                                  >  1:75876/1‑139 (MQ=255)

                                     aGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGcac                                                                                                  <  2:2149877/139‑1 (MQ=39)

                                      gCAGAAGCGCGACATCGTTGCCCTGTAAGACCGCAAGTAAGCTGCAGTTGCCGAACGCAAGAAGGCTGCCCTCGGGCTGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTGCCCGCGAAG‑AGGCCGGcacc                                                                                                 >  2:6878326/1‑138 (MQ=255)

                                                  cATCGTGGCCCTGAAAGACCGGATGTAAGATGCTGTAGCCGAACGCATGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTCTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACaaaa                                                                                     <  2:5815025/139‑1 (MQ=37)

                                                  aaTCGTGGCCCTGAAAGACCGAAAGTAAGCTCCAGATGCCGAACGCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCGTGTCCCGGCATCGACGCGCCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACaaaa                                                                                     <  2:7363368/138‑1 (MQ=255)

                                                   aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCTGCC‑TTTTTTATTTGACCTGTGCGGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAAc                                                                                    <  2:6787397/139‑1 (MQ=255)

                                                       ttGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGGACAGACACCACAAAACCTCa                                                                                >  2:4255536/1‑139 (MQ=39)

                                                       ttGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCa                                                                                >  2:9092884/1‑139 (MQ=39)

                                                          cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAACACCTccctt                                                                             >  2:7453968/1‑135 (MQ=39)

                                                          cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTGCCCGCGAAG‑ACGCCGGGACAGACACCACAAAACCTCAGtt                                                                             >  2:6590831/1‑139 (MQ=255)

                                                                 aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CGTTTTTTATTTGTCCTGTGCCGGCCACTTCGGGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                                      <  1:2992259/139‑1 (MQ=255)

                                                                  gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑TGGCCGGCACACACACCACAAAACCTCAGTTTTTCTacg                                                                     >  2:2535196/1‑139 (MQ=255)

                                                                  gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCCGGCTTGCCCGCGAAG‑AGGCCGGCACACACACCAAAAAACCTCAGTTTTTCTacg                                                                     >  2:6465936/1‑139 (MQ=38)

                                                                   aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc                                                                    <  2:2802201/139‑1 (MQ=255)

                                                                   aCCGCAAGTAAGCATCAGTAGCCGAACCCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTGCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc                                                                    <  1:6582783/139‑1 (MQ=255)

                                                                    ccGAAAGTAAGCTGATGAACCCGAACCAAATAAGGCTGCCCTCGGGCAG‑CTTTTTTTTTTTTTCCTGTGCCGGCATCTTCGGGGCCTTGCCCGCCATG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcc                                                                   <  1:8694449/139‑1 (MQ=255)

                                                                        aattAAGGTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCGGGCCACTGCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                               <  1:1607950/136‑1 (MQ=255)

                                                                        aaGTAGCCTGCAGACCCCAAACGAAAGAGGGCGCCCCGCGGGCCG‑CTTTTTTTTTTTGTCCGGGCCCGCCCACTCCGCGGCCAGCCCCGCCAAG‑CGCCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                               <  1:6063600/139‑1 (MQ=255)

                                                                        aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAGG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                               <  1:9410694/139‑1 (MQ=255)

                                                                        aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                               <  1:4221141/139‑1 (MQ=39)

                                                                         aGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTc                                                              >  2:2919237/1‑139 (MQ=39)

                                                                         aGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCACCGCCCGCTc                                                              >  2:8708547/1‑139 (MQ=39)

                                                                         aGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCCGCCCAGACACCACAAAACCTCACTTTTTATCCGCCCGCTc                                                              >  2:8354283/1‑139 (MQ=255)

                                                                           tAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                                            <  1:1259877/139‑1 (MQ=39)

                                                                           tAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTGGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                                            <  1:2651900/139‑1 (MQ=255)

                                                                           tAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                                            <  2:1333640/139‑1 (MQ=255)

                                                                            acggtGCAGTAGCCTAACCAAAGACGGCGGCCGGGGGGCAGCGTTTTTTTTTTGACCTGG‑CCCGGCCTCTTCGCGGCCTGCCCCGCGAAG‑AGCCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                           <  1:8480884/135‑1 (MQ=255)

                                                                            aaGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                                            >  2:4750201/1‑138 (MQ=39)

                                                                            aaGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                           <  1:2377604/139‑1 (MQ=39)

                                                                             aGCTGCTGTAGCCGAACGCAAGAAGGCTGCCCTGGGGCAG‑CGTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc                                                          <  2:6353585/139‑1 (MQ=38)

                                                                             aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTACGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc                                                          <  1:4801347/139‑1 (MQ=255)

                                                                                  gaGTAGCCGAACGGAAGAAGGCTGCCGGCGGGCAG‑CGTTTTTTATTTGACCTGTGCCGGCCTCTTCGCGGCCTGGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                     <  1:299768/138‑1 (MQ=255)

                                                                                  cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                     <  1:4760960/139‑1 (MQ=39)

                                                                                  cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                     <  1:1606560/139‑1 (MQ=39)

                                                                                  cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                     <  1:2042055/139‑1 (MQ=39)

                                                                                  cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                     >  1:2171879/1‑139 (MQ=39)

                                                                                  cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCATGCCCGCCAAC‑AGGCCGGCCCACACACCACAAAACCACACTTTTTCTACGCCCCCTCACCCAcccc                                                     >  1:3766435/1‑139 (MQ=255)

                                                                                  cAGTAGCCGAACGCAAGAAGGCTCCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCCCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                     <  2:8193759/139‑1 (MQ=255)

                                                                                  cAGTAGCCGAACGCAAGAAGCCTGCCCTCGGGCAGNGTTTTTTT‑TTTGTCGTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                     <  1:5352219/139‑1 (MQ=39)

                                                                                    gTAGCCGACCGCAAGAAGGCTGCCCTCGGGCAGCC‑TATTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGACG‑AGGCCGGCACAGACTCCACAAAACCTCACTTTTTCTCCGCCCGCTCACGCAGCCGtt                                                   >  2:1891350/1‑139 (MQ=255)

                                                                                    gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  2:1230866/139‑1 (MQ=39)

                                                                                    gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:6975265/139‑1 (MQ=39)

                                                                                    gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:4010430/139‑1 (MQ=39)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCTGCC‑TTTTTTATTTGTCCTGTGCCGGCATCTTCGCGGCGTTGGCCGCGTAG‑AGGGCGGGACACACACCACAAAACCTCATTATTTCTCCGCCCGCTCACGCAGCCCtct                                                  >  2:7009454/1‑137 (MQ=255)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGGCTCATCGCGGGCATTCCCCCGCAC‑AGGGCGGCCCAGAAACCAAAAAACCTCATTTTTTATACCCCCCCGCACCCCGcccaat                                                  >  1:6699886/1‑136 (MQ=255)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCttt                                                  >  2:6161419/1‑139 (MQ=39)

                                                                                     tAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCCGTGGCGGGCGTGCCCGCGCAC‑AGGGCGGGCCAGACACCACAAAAACACACTTTTTTTGCGCCCGCGCACCCACCCCatt                                                  >  1:5144345/1‑139 (MQ=255)

                                                                                     aaccccaaCCCAAAAAGGCAGCCCCGGGGCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCTACTGGGGGCCTTGCCCGCGTAGCAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCtt                                                   <  1:5622530/133‑1 (MQ=255)

                                                                                         cGAACGCAAGAAGGCTGCCCACGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                              <  1:1135420/139‑1 (MQ=39)

                                                                                          gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCGTGCCCGCGAAG‑AGGCCGGCACAGTCACCACAAAACCACAGTTTTTCTCCGCCCGCTCACGCACCCCATTCAcgg                                             >  1:1755/1‑139 (MQ=37)

                                                                                          gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                                 <  1:342788/72‑1 (MQ=37)

                                                                                          gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcgc                                                                                                                 >  2:342788/1‑72 (MQ=37)

                                                                                          gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCATGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAggg                                             >  1:7920/1‑139 (MQ=255)

                                                                                             cGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt                                          >  2:5764624/1‑139 (MQ=39)

                                                                                             cGCAAGAACGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGGCGGCCTCTTCGCGGCCTAGCCCGCGAAG‑AGGCCGGCTCTGACACCACTAAACCTCAGTATTACTCCTCCCGCTGACCCAGCCCGTTCAGGGTAt                                          >  2:2210671/1‑139 (MQ=255)

                                                                                               cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑TGGCCGGCACAGACACCACAAAACCTCAGTTTTTCACCGCCCGCTCACGCAGCCCTATCAGGGTATTg                                        >  2:9447769/1‑139 (MQ=255)

                                                                                               cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTg                                        <  2:2988069/139‑1 (MQ=39)

                                                                                               cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTg                                        <  2:502673/139‑1 (MQ=39)

                                                                                               cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCACTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTg                                        >  2:5953447/1‑139 (MQ=39)

                                                                                                 agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGaa                                      <  2:7893715/139‑1 (MQ=39)

                                                                                                     ggCTGCCCTCGGCCAGCCTTTTTTT‑TTTGTCGTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                                  <  1:6215774/139‑1 (MQ=39)

                                                                                                             tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGTCACCACAAAACCTCAGTTTTTCTCCGCCCGCTCCCGCAGCCCTTTCAGGGTATTGAAAGGCGCATccac                          >  1:3549893/1‑139 (MQ=38)

                                                                                                             tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                          >  2:3640943/1‑139 (MQ=39)

                                                                                                             tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                          >  2:2769010/1‑139 (MQ=39)

                                                                                                             tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                          >  2:100201/1‑139 (MQ=39)

                                                                                                             tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                          >  2:4021322/1‑139 (MQ=39)

                                                                                                             tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCGTGGCCCCGAAG‑AGGGCGGGCCAGACACCACAAAACCTCACTTTTTCTCCGCCCGCTGACTCAGCCCCTTCAGCGTTTTGAACCGCGCCTccac                          >  2:7676101/1‑139 (MQ=255)

                                                                                                                 gCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACg                      <  2:3162729/139‑1 (MQ=39)

                                                                                                                        ttttttATTTGTCCTGTGCCGGCCTCTTCGCGCCCTTGCCCGCGAAG‑AGGCCGGAACAGACACCACAACACCTCAGATTATCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACCAACttg                >  2:1822015/1‑139 (MQ=255)

||

TACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAG‑AGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGC  >  AE015451_phaM1/4740686‑4740958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A