Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F6 I1 R1 81 160.8 7353682 98.1% 7213962 137.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 5,701,209 C→A intergenic (+1955/‑2050) PP_5002 CDS (conserved protein of unknown function) → / → PP_5009 CDS (conserved protein of unknown function) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,701,2090CA100.0% 637.4 / NA 157intergenic (+1955/‑2050)PP_5002 CDS (conserved protein of unknown function)/PP_5009 CDS (conserved protein of unknown function)–/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (60/97);  total (60/97)

Only 100 of 157 total aligned reads displayed.

CCGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGCGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAGCC  >  AE015451_phaM1/5701079‑5701336
                                                                                                                                  |                                                                                                                               
 cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAAc                                                                                                                        <  1:2911700/139‑1 (MQ=255)
 cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAAc                                                                                                                        <  1:2106874/139‑1 (MQ=255)
 cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAAc                                                                                                                        <  1:1523156/139‑1 (MQ=255)
 cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAAc                                                                                                                        <  1:2106678/139‑1 (MQ=255)
 cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAAc                                                                                                                        <  1:2976949/139‑1 (MQ=255)
 cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAAc                                                                                                                        <  1:2059223/139‑1 (MQ=255)
 cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAAc                                                                                                                        <  1:2976960/139‑1 (MQ=255)
 cGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAAc                                                                                                                        <  1:3092407/139‑1 (MQ=255)
         cGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGAt                                                                                                                <  2:231146/139‑1 (MQ=255)
         cGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGAt                                                                                                                <  2:1575809/139‑1 (MQ=255)
         cGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGAt                                                                                                                <  2:1575725/139‑1 (MQ=255)
             cTATGCCGGTCTGCGCTCGGCGGCCCTCCACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTAc                                                                                                            >  1:86131/1‑139 (MQ=255)
              tATGCCGGTCTTCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACg                                                                                                           <  2:2020266/139‑1 (MQ=255)
              tATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACg                                                                                                           <  2:2023412/139‑1 (MQ=255)
              tATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACg                                                                                                           <  2:1309895/139‑1 (MQ=255)
                   cggcatGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  1:625025/134‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGGGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  2:792428/139‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  1:3441291/139‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  2:2372837/139‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  2:2371752/139‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  2:2266272/139‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  2:942177/139‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  1:154705/139‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  1:1866389/139‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  1:153833/139‑1 (MQ=255)
                   cGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACg                                                                                                      <  1:2067182/139‑1 (MQ=255)
                       cTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGa                                                                                                  <  2:557375/139‑1 (MQ=255)
                         gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAg                                                                                                >  2:503762/1‑139 (MQ=255)
                         gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAg                                                                                                >  2:668467/1‑139 (MQ=255)
                         gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAg                                                                                                >  1:22255/1‑139 (MQ=255)
                         gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAg                                                                                                >  2:2318726/1‑139 (MQ=255)
                         gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAg                                                                                                >  2:3152549/1‑139 (MQ=255)
                         gcgcTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAg                                                                                                >  1:2971656/1‑139 (MQ=255)
                         ccccTCGGCGCCCATCAACCTCTACGTCAACGCCAAGGGCCTCGACGACAGCGCCTTCGCCGAGGACCGGCTGAAGGAGCTGGAAGGCCTCCTGGCCGACGCGGGAGCGCTCAACGAGCGGAACTAGGAGACGGTGAAg                                                                                                >  2:708310/4‑139 (MQ=255)
                             tCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCa                                                                                            >  1:367465/1‑139 (MQ=255)
                             tCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCa                                                                                            >  1:367020/1‑139 (MQ=255)
                             tCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCa                                                                                            >  2:3505490/1‑139 (MQ=255)
                                 ctgcCCTGAACGTCTACGTCAACCCCAAGGGCCTCGACGACCGCGCCTTCGCCGTGGAGCCGCTGAAGGACCTGGATGGCCTGCTGGCCGAGGCGGGAGCGCTCAACCAGCGGATCTACGAGACCGTGAAGTCCAAGGt                                                                                        <  2:2100508/137‑1 (MQ=255)
                                 cggcCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGATCGGCTGAAGGAGCTGGATGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGt                                                                                        <  2:1907946/139‑1 (MQ=255)
                                 cggcCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGt                                                                                        <  1:2538869/139‑1 (MQ=255)
                                 cggcCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGt                                                                                        <  1:2625349/139‑1 (MQ=255)
                                   gcCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTaa                                                                                      <  2:1758338/139‑1 (MQ=255)
                                   gcCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTaa                                                                                      <  2:3397933/139‑1 (MQ=255)
                                   gcCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTaa                                                                                      <  2:2903389/139‑1 (MQ=255)
                                         aaCGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGAc                                                                                <  1:2556267/139‑1 (MQ=255)
                                           cGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGATGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAATTCCAAGGTAAACTGACCt                                                                              <  1:1688768/139‑1 (MQ=255)
                                                 cGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGAt                                                                        >  2:281041/1‑139 (MQ=255)
                                                   tCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGAttt                                                                      <  2:2669831/139‑1 (MQ=255)
                                                         ccAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTc                                                                >  1:297771/1‑139 (MQ=255)
                                                         ccAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTc                                                                >  1:1939290/1‑139 (MQ=255)
                                                         ccAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTc                                                                >  1:1368055/1‑139 (MQ=255)
                                                         ccAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTc                                                                >  1:2841664/1‑139 (MQ=255)
                                                         ccAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTc                                                                >  1:171911/1‑139 (MQ=255)
                                                         ccAAGAGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGTAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTc                                                                >  1:1372639/1‑139 (MQ=255)
                                                          caaccgCCTCGACGACCCCGCCTTCGCACAGGAGCGGCTGAAGGAGCTGGAAGCCCTGCTGGCCGAGGCGCGAGCGCTCAACGAGCGGATCTACGAGACGGTGAACTCCAAGGTAAACTGAGCTAGGGATTTTAATTCa                                                               >  2:3324028/6‑139 (MQ=255)
                                                          cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGACGTCCAAGGTAACCTGACGTAGGGATTTTAATTCa                                                               >  2:2073043/1‑139 (MQ=255)
                                                          cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCa                                                               >  2:1714846/1‑139 (MQ=255)
                                                          cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCa                                                               >  1:3539712/1‑139 (MQ=255)
                                                          cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCa                                                               >  1:1074842/1‑139 (MQ=255)
                                                          cAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTACGGATTTTAATTCa                                                               >  2:3512580/1‑139 (MQ=255)
                                                              ggCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAg                                                           <  1:3109474/139‑1 (MQ=255)
                                                                ccTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCa                                                         <  1:3490523/139‑1 (MQ=255)
                                                                ccTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCa                                                         <  1:2333840/139‑1 (MQ=255)
                                                                      cgacCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTc                                                   >  1:813455/1‑139 (MQ=255)
                                                                      cgacCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTc                                                   >  1:334705/1‑139 (MQ=255)
                                                                      cgacCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTc                                                   >  1:2805409/1‑139 (MQ=255)
                                                                        acCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCtt                                                 <  1:3558368/139‑1 (MQ=255)
                                                                        acCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCtt                                                 <  1:2004359/139‑1 (MQ=255)
                                                                        acCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCtt                                                 <  1:2111516/139‑1 (MQ=255)
                                                                         ccGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCttt                                                <  1:574633/139‑1 (MQ=255)
                                                                         ccGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCttt                                                <  1:1560765/139‑1 (MQ=255)
                                                                          cgcgCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTg                                               <  2:1030360/139‑1 (MQ=255)
                                                                           gcgcCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGt                                              <  1:2656821/139‑1 (MQ=255)
                                                                           gcgcCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGt                                              <  1:2657273/139‑1 (MQ=255)
                                                                            cgcCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTa                                             >  2:461425/1‑139 (MQ=255)
                                                                               cTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGc                                          <  2:22255/139‑1 (MQ=255)
                                                                               cTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGc                                          <  1:1083231/139‑1 (MQ=255)
                                                                               cTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGc                                          >  2:3431763/1‑139 (MQ=255)
                                                                               cTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGc                                          <  1:461425/139‑1 (MQ=255)
                                                                               cTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGc                                          <  1:461453/139‑1 (MQ=255)
                                                                               cTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGc                                          <  2:2578796/139‑1 (MQ=255)
                                                                               cTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCGTTGTATGc                                          <  2:2971656/139‑1 (MQ=255)
                                                                                ttCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGcc                                         <  1:2340045/139‑1 (MQ=255)
                                                                                ttCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGcc                                         <  1:2340073/139‑1 (MQ=255)
                                                                                               cTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGAt                          >  2:2234341/1‑139 (MQ=255)
                                                                                               cTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGAt                          >  2:3529987/1‑139 (MQ=255)
                                                                                               cTGAAGGAGCTGGAAGGCCAGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCCAGAt                          >  1:639198/1‑139 (MQ=255)
                                                                                                        cTGGAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCcgcgc                 <  1:1664011/139‑1 (MQ=255)
                                                                                                          ggAAGGCCTGCTGGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCgcgg               >  1:1466089/1‑139 (MQ=255)
                                                                                                                   gctgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTgn      >  1:3361374/1‑138 (MQ=255)
                                                                                                                   gctgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTgn      >  1:3361560/1‑138 (MQ=255)
                                                                                                                     tgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGTAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAg    <  2:2737048/139‑1 (MQ=255)
                                                                                                                     tgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAACCTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAg    >  2:2894280/1‑139 (MQ=255)
                                                                                                                     tgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAg    >  2:2592926/1‑139 (MQ=255)
                                                                                                                     tgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAg    >  2:1073806/1‑139 (MQ=255)
                                                                                                                     tgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAg    >  2:1959455/1‑139 (MQ=255)
                                                                                                                     tgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAg    <  2:832603/139‑1 (MQ=255)
                                                                                                                     tgGCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAg    <  2:2737453/139‑1 (MQ=255)
                                                                                                                      ggCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAGc   <  2:1729623/139‑1 (MQ=255)
                                                                                                                       gCCGAGGCGGGAGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAGcc  >  1:1927495/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                               
CCGTGCTCTCGGCCTATGCCGGTCTGCGCTCGGCGGCCCTCAACGTCTACGTCAACGCCAAGGGCCTCGACGACCGCGCCTTCGCCGAGGAGCGGCTGAAGGAGCTGGAAGGCCTGCTGGCCGAGGCGGGCGCGCTCAACGAGCGGATCTACGAGACGGTGAAGTCCAAGGTAAACTGACCTAGGGATTTTAATTCATTAGCAGGAGTCTTTGTATGCCCTCAGATATCGAGATCGCCCGCGCGGCGACCCTGAAGCC  >  AE015451_phaM1/5701079‑5701336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A