Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A20 F4 I1 R1 86 415.0 19624302 98.4% 19310313 137.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
MC JC AE015451_phaM1 5,918,836 Δ4,582 bp [gcvP‑II CDS (glycine dehydrogenase)]–[gcvT‑II CDS (aminomethyltransferase)] [gcvP‑II CDS (glycine dehydrogenase)], gcvH‑II CDS (glycine cleavage system H protein 2), [gcvT‑II CDS (aminomethyltransferase)]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ AE015451_phaM1 5918836 5923417 4582 493 [0] [0] 489 [gcvP‑II CDS (glycine dehydrogenase)]–[gcvT‑II CDS (aminomethyltransferase)] [gcvP‑II CDS (glycine dehydrogenase)],gcvH‑II CDS (glycine cleavage system H protein 2),[gcvT‑II CDS (aminomethyltransferase)]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451_phaM1 = 59188350 (0.000)489 (1.110) 93/272 0.1 100% coding (2872/2874 nt) gcvP‑II CDS (glycine dehydrogenase)
?AE015451_phaM1 5923418 = 0 (0.000)coding (3/1083 nt) gcvT‑II CDS (aminomethyltransferase)

GATK/CNVnator alignment

N/A