Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F6 I2 R1	82	424.1	19537984	98.2%	19186300	137.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	5,674,753	G→C	intergenic (+115/+25)	rhlE‑II CDS (ATP‑dependent RNA helicase) → / ← yceI CDS (polyisoprene‑binding protein_ acidic stress response factor)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	5,674,753	0	G	C	99.6%	1835.7 / NA	454	intergenic (+115/+25)	rhlE‑II CDS (ATP‑dependent RNA helicase)/yceI CDS (polyisoprene‑binding protein_ acidic stress response factor)	–/–
Reads supporting (aligned to +/- strand): ref base G (2/0); new base C (259/192); total (262/192)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 455 total aligned reads displayed.

CGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTG  >  AE015451_phaM1/5674616‑5674880

  ctgctgACCCGCAAGGGCTGATAGAAGTACCGACCGGGCTCCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCACAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCTAA‑GC‑GGGccc                                                                                                                              >  2:2682322/1‑139 (MQ=38)

        aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGt                                                                                                                         >  2:5545523/1‑139 (MQ=255)

        aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGt                                                                                                                         >  1:6962950/1‑139 (MQ=255)

        aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGt                                                                                                                         >  1:6565276/1‑139 (MQ=255)

                gggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCt                                                                                                                 >  2:660749/1‑139 (MQ=255)

                 ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTg                                                                                                                <  2:5726526/139‑1 (MQ=255)

                 ggCTGATAGAACTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTg                                                                                                                <  2:1492262/139‑1 (MQ=255)

                      aTAGAAGAACGGAGGGGGCTGATTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCATAGATTTTCTTGATCTCTAGAGGAGCGGCCTGGTGTCGAGATAGGGGCGCAAA‑GCGGCCCCCTCGGTTTTGAGCTGGCAgt                                                                                                           <  2:5358563/139‑2 (MQ=25)

                           aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGATTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTa                                                                                                      <  1:498401/139‑1 (MQ=255)

                           aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGCCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTa                                                                                                      <  2:2171076/139‑1 (MQ=255)

                               ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                  >  1:4258039/1‑139 (MQ=255)

                               ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTAAGCTGGCAGAAATTACTTc                                                                                                  >  2:1078323/1‑139 (MQ=255)

                                   gggggCTGCTTCGCAGCCCTATCACGACACCAGGCCGCTCCTGCAGAGAATTACTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAC‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAATTTACTTCTGCt                                                                                              >  2:5994700/1‑139 (MQ=37)

                                   gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGTATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCt                                                                                              >  1:2626175/1‑139 (MQ=255)

                                   gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGGAGAAATTACTTCTGCt                                                                                              >  1:7158172/1‑139 (MQ=255)

                                   gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCt                                                                                              >  2:2048370/1‑139 (MQ=255)

                                   gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTCGCAGAAATTACTTCTGCt                                                                                              >  1:544385/1‑139 (MQ=255)

                                    ggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                             >  1:488602/1‑139 (MQ=255)

                                    ggggCTCCTTCGCAGCCGTAACACGACACAAGGCCGCTCCTGCTGAGAATTTCGAGATCTCTACAGCAGCGGCGTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAAATACTTCAGCta                                                                                             <  2:8363639/139‑2 (MQ=25)

                                       gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCACTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAACTTACTTCTGCTTcac                                                                                          >  2:8024585/1‑139 (MQ=37)

                                          gctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                       >  1:2520678/1‑139 (MQ=255)

                                          gctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                       >  2:5156737/1‑139 (MQ=255)

                                            ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                     <  2:6971045/139‑1 (MQ=255)

                                            ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                     >  1:3443639/1‑139 (MQ=255)

                                            ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                     <  2:3871566/139‑1 (MQ=255)

                                            ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                     >  2:5277886/1‑139 (MQ=255)

                                              cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCg                                                                                   <  2:3769779/139‑1 (MQ=255)

                                                 aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca                                                                                <  2:2248620/139‑1 (MQ=255)

                                                 aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca                                                                                >  2:2458874/1‑139 (MQ=255)

                                                 aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca                                                                                >  2:8729740/1‑139 (MQ=255)

                                                      tATCACGACACAAGGGCGCTCCTGCAGAGTATTTCTTGAACTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat                                                                           <  1:6413862/139‑1 (MQ=38)

                                                      tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat                                                                           <  1:4057951/139‑1 (MQ=255)

                                                      tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat                                                                           <  1:3896010/139‑1 (MQ=255)

                                                      tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat                                                                           <  2:127625/139‑1 (MQ=255)

                                                       aTCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGata                                                                          <  2:8508162/139‑1 (MQ=255)

                                                           cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcca                                                                      >  2:4767288/1‑139 (MQ=255)

                                                           cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcca                                                                      >  2:9590178/1‑139 (MQ=255)

                                                             acacAAGCCCCCTCCTCCACAGAATTACTGGATAACTACAGGAGCGCCCTTGTGACGTCATAGGCCCCCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccagg                                                                    <  2:4170385/139‑1 (MQ=255)

                                                                caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcc                                                                 >  1:2159198/1‑139 (MQ=255)

                                                                   ggCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                     <  2:3247278/116‑1 (MQ=255)

                                                                   ggCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                     >  1:3247278/1‑116 (MQ=255)

                                                                    gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGat                                                                           <  2:7524457/125‑1 (MQ=255)

                                                                     ccccTCCAGCAGAGAATATCTAGTACTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                            <  2:7597597/136‑1 (MQ=37)

                                                                     ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                            <  1:8513523/139‑1 (MQ=255)

                                                                     ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                            <  1:3141844/139‑1 (MQ=255)

                                                                     ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                            <  1:367318/139‑1 (MQ=255)

                                                                     ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                            <  1:5338670/139‑1 (MQ=255)

                                                                     ccGCACCTGCAGAGAATTTCTTGATCTCTTCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAAATACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                            <  2:6801908/139‑1 (MQ=38)

                                                                     ccGCACCTGCAGAGAATTTCTTGATCACTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                            <  1:9389846/139‑1 (MQ=39)

                                                                           cTGCAGAGAATTTCTTGATCTCTACAGGAGGGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                      >  2:8828206/1‑139 (MQ=255)

                                                                           cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                      >  2:8886863/1‑139 (MQ=255)

                                                                           cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                      >  1:5801569/1‑139 (MQ=255)

                                                                           cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                      >  2:5803799/1‑139 (MQ=255)

                                                                           cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                      >  1:6255465/1‑139 (MQ=255)

                                                                           cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                      >  2:1122756/1‑139 (MQ=255)

                                                                           cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                      <  1:6842765/139‑1 (MQ=255)

                                                                                 agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCGCCTCGGTTTTCAGCTGGCAGAACGTACTTCTGCTTCACTCCTTGGACACTGATATCCAGGACCAGGGACTGCGAAGTAGc                                                >  2:9326431/1‑139 (MQ=37)

                                                                                 agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGc                                                <  2:488602/139‑1 (MQ=255)

                                                                                           gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt                                      >  2:4306915/1‑139 (MQ=255)

                                                                                           gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt                                      >  1:810773/1‑139 (MQ=255)

                                                                                           gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt                                      >  1:8231526/1‑139 (MQ=255)

                                                                                           gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt                                      >  1:7626334/1‑139 (MQ=255)

                                                                                               tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGa                                  <  1:8729647/139‑1 (MQ=255)

                                                                                                ctgcAGGAGCGGCCTTGTGTCGTGATAGGGCTGCGAA‑GCAGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                 >  1:3526443/4‑139 (MQ=37)

                                                                                                ctgcAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                 >  2:9434303/4‑139 (MQ=255)

                                                                                                ctgcAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                 >  2:7824812/4‑139 (MQ=255)

                                                                                                ctgcAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                 >  1:1338834/4‑139 (MQ=255)

                                                                                                ctgcAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                 >  2:4537999/4‑139 (MQ=255)

                                                                                                ctgcAGGAGCGACCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCCAAGTAGCACCCGGGCCTTTGAt                                 >  1:1722722/4‑139 (MQ=38)

                                                                                                    aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                             >  2:4070882/1‑139 (MQ=255)

                                                                                                    aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                             >  2:720233/1‑139 (MQ=255)

                                                                                                         cGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                        <  1:1946499/139‑1 (MQ=255)

                                                                                                         cGGCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                        <  1:9605348/139‑1 (MQ=255)

                                                                                                          ggCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg                       <  1:1577543/139‑1 (MQ=255)

                                                                                                          ggCCTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg                       <  1:2904629/139‑1 (MQ=255)

                                                                                                            ccTTGTGTCGTGTTAGGGCCGCAAAGGG‑GCCGCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCATCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                     >  2:6522026/1‑139 (MQ=255)

                                                                                                            ccTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                     >  2:4973663/1‑139 (MQ=255)

                                                                                                            ccTTGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                     >  2:7432129/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  1:8840460/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  1:1030559/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  2:4471361/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  2:3386725/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  2:9207123/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  2:3001202/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  2:8665261/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  1:5115694/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  1:5914646/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  2:1855851/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  2:7319600/1‑139 (MQ=255)

                                                                                                              ttGTGTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                   >  2:7133666/1‑139 (MQ=255)

                                                                                                               tgtgTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                  <  1:4750868/139‑1 (MQ=255)

                                                                                                               tgtgTCGTGATAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                  <  1:225335/139‑1 (MQ=255)

                                                                                                                         tAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                  >  2:6360514/1‑129 (MQ=255)

                                                                                                                         tAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg        >  1:6969600/1‑139 (MQ=255)

                                                                                                                         tAGGGCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg        >  1:7299948/1‑139 (MQ=255)

                                                                                                                            ggCCGCAAACCC‑GCCCCCACGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGATGTAGCACCCGGGCCTTTGATGCCGTAGTCGTTCTGGTTCAGCgtggtg     <  1:5730315/139‑1 (MQ=255)

                                                                                                                            ggCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg     <  2:1970579/139‑1 (MQ=255)

                                                                                                                            ggCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg     <  1:7878367/139‑1 (MQ=255)

                                                                                                                            ggCCGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg     <  1:9133113/139‑1 (MQ=255)

                                                                                                                               cGCAAA‑GCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg  >  2:6930922/1‑139 (MQ=255)

CGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAA‑GC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTG  >  AE015451_phaM1/5674616‑5674880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTC  >  AE015451_phaM1/5681268‑5681566

TTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggg                                                                                                                                                        <  VH01111:79:AACJV3JHV:1:1410:47316:11564/153‑6 (MQ=60)

TTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggg                                                                                                                                                        <  VH01111:79:AACJV3JHV:1:2105:46540:38390/153‑6 (MQ=60)

TTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggg                                                                                                                                                        <  VH01111:79:AACJV3JHV:1:2105:46748:38447/153‑6 (MQ=60)

TTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggg                                                                                                                                                        <  VH01111:79:AACJV3JHV:2:2111:19651:51908/153‑6 (MQ=60)

TTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggg                                                                                                                                                        <  VH01111:79:AACJV3JHV:2:1212:23988:36630/153‑6 (MQ=60)

 TCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggc                                                                                                                                                       <  VH01111:79:AACJV3JHV:1:1408:32471:26406/153‑7 (MQ=60)

 TCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggc                                                                                                                                                       <  VH01111:79:AACJV3JHV:2:1108:28400:6661/153‑7 (MQ=60)

 TCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggc                                                                                                                                                       <  VH01111:79:AACJV3JHV:1:2114:35747:14915/153‑7 (MQ=60)

  CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggct                                                                                                                                                      <  VH01111:79:AACJV3JHV:2:1508:42544:12813/153‑8 (MQ=60)

  CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggct                                                                                                                                                      <  VH01111:79:AACJV3JHV:2:1513:57844:12094/153‑8 (MQ=60)

  CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggct                                                                                                                                                      <  VH01111:79:AACJV3JHV:2:2605:39004:22374/153‑8 (MQ=60)

  CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggct                                                                                                                                                      <  VH01111:79:AACJV3JHV:2:2605:24556:24589/153‑8 (MQ=60)

  CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggct                                                                                                                                                      <  VH01111:79:AACJV3JHV:2:1513:60325:12795/153‑8 (MQ=60)

  CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggct                                                                                                                                                      <  VH01111:79:AACJV3JHV:2:2301:27131:46853/153‑8 (MQ=60)

    CCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggggtgc                                                                                                                                                    >  VH01111:79:AACJV3JHV:1:2609:63241:28243/1‑144 (MQ=60)

    CCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggggggg                                                                                                                                                    >  VH01111:79:AACJV3JHV:2:2502:46824:50298/1‑144 (MQ=60)

    CCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgc                                                                                                                                                    >  VH01111:79:AACJV3JHV:1:2609:63790:28224/1‑144 (MQ=60)

      ggagatgtgtataagagacagCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTG                                                                                                                                                  <  VH01111:79:AACJV3JHV:2:1214:40178:27789/132‑1 (MQ=60)

      ggagatgtgtataagagacagCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTG                                                                                                                                                  <  VH01111:79:AACJV3JHV:1:2313:51539:42215/132‑1 (MQ=60)

         ATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                               <  VH01111:79:AACJV3JHV:1:2114:44287:35191/153‑1 (MQ=60)

         ATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                               <  VH01111:79:AACJV3JHV:2:1209:69830:31234/153‑1 (MQ=60)

         ATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                               <  VH01111:79:AACJV3JHV:2:2406:27226:13173/153‑1 (MQ=60)

         ATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                               <  VH01111:79:AACJV3JHV:1:1302:76022:41211/153‑1 (MQ=60)

         ATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGCCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                               <  VH01111:79:AACJV3JHV:2:2406:27604:12416/153‑1 (MQ=60)

         ATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                               <  VH01111:79:AACJV3JHV:1:2206:50535:11678/153‑1 (MQ=60)

            CATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCACC                                                                                                                                            >  VH01111:79:AACJV3JHV:1:1603:59170:40605/1‑153 (MQ=60)

              TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCC                                                                                                                                          <  VH01111:79:AACJV3JHV:2:1109:62294:29152/153‑1 (MQ=60)

              TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCC                                                                                                                                          <  VH01111:79:AACJV3JHV:2:1109:60514:30628/153‑1 (MQ=60)

              TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCC                                                                                                                                          <  VH01111:79:AACJV3JHV:1:2102:57750:10863/153‑1 (MQ=60)

              TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCC                                                                                                                                          <  VH01111:79:AACJV3JHV:2:1302:14634:5581/153‑1 (MQ=60)

              TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCC                                                                                                                                          <  VH01111:79:AACJV3JHV:1:2405:39609:17187/153‑1 (MQ=60)

              TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCC                                                                                                                                          <  VH01111:79:AACJV3JHV:1:2302:71989:44411/153‑1 (MQ=60)

                 AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT                                                                                                                                       <  VH01111:79:AACJV3JHV:2:2308:30729:50601/153‑1 (MQ=60)

                   tcggagatgtgtataagagacagGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:1:1607:31865:52230/130‑1 (MQ=60)

                   tcggagatgtgtataagagacagGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:1:1303:26355:18323/130‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:1:2613:46691:29909/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:1:1104:66195:9993/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:2:2608:30445:28394/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:1:1205:20731:13003/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:1:1212:34724:50734/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:2:1105:37621:23945/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:2:1105:37205:24627/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:2:1604:69906:12605/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:2:2507:34289:54123/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:2:2406:58280:53309/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:1:1507:11547:29417/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:1:2411:43700:4654/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:2:1604:69736:12094/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCATGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:2:1107:49115:28887/153‑1 (MQ=60)

                   ACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCATGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                     <  VH01111:79:AACJV3JHV:2:2107:48490:29701/153‑1 (MQ=60)

                         ACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACAC                                                                                                                               <  VH01111:79:AACJV3JHV:1:1611:25275:9292/153‑1 (MQ=60)

                         ACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACAC                                                                                                                               <  VH01111:79:AACJV3JHV:1:1607:32736:45944/153‑1 (MQ=60)

                          CCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGGCTTGTGTCGCGCAAGGGGCGCCAAGGCGCCCCACGCTTTCCTTGCCACTCCCTGAACCGCcggggcggctgcggccgccaatccccccaca                                                                                                                              >  VH01111:79:AACJV3JHV:1:2313:60363:47591/1‑122 (MQ=60)

                              gagatgtgtataagagacagGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:1509:34838:21617/133‑1 (MQ=60)

                              gagatgtgtataagagacagGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:1509:31751:26217/133‑1 (MQ=60)

                              gagatgtgtataagagacagGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:2306:13800:43180/133‑1 (MQ=60)

                              CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:1114:39534:8630/153‑1 (MQ=60)

                              CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:2:1201:43359:9690/153‑1 (MQ=60)

                              CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:1201:39344:42480/153‑1 (MQ=60)

                              CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:2204:51349:40738/153‑1 (MQ=60)

                              CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:1506:41219:16789/153‑1 (MQ=60)

                              CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:1506:41768:17149/153‑1 (MQ=60)

                              CTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                          <  VH01111:79:AACJV3JHV:1:2204:51936:41514/153‑1 (MQ=60)

                               TGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCC                                                                                                                         <  VH01111:79:AACJV3JHV:2:2402:62389:29208/153‑1 (MQ=60)

                                GAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGGCTTGTGTCGCGAAACGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgctgcccgcaaatcagaacaaccat                                                                                                                        >  VH01111:79:AACJV3JHV:2:2109:68903:50071/1‑116 (MQ=60)

                                       GCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcctgtctcttatacacatctccgagcccacg                                                                                                                 >  VH01111:79:AACJV3JHV:1:1309:41617:6244/1‑109 (MQ=60)

                                       GCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcctgtctcttatacacatctccgagcccacg                                                                                                                 >  VH01111:79:AACJV3JHV:2:1609:28324:50393/1‑109 (MQ=60)

                                                 gcagtctcgtgggctcggagatgtgtataagagacaGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCAC                                                                                                       <  VH01111:79:AACJV3JHV:1:2609:67312:14252/117‑1 (MQ=60)

                                                                             GTGGGAGCGGCCATGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT                                                                                                                                       <  VH01111:79:AACJV3JHV:1:1508:45120:31707/93‑1 (MQ=60)

                                                                                   GCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGGGCAGCCCTATAGCGACACAAGGCCGCTCCCACACCAGACCACCAGGCCCAGCGCCACACATacaaaacaagctcag                                                                     >  VH01111:79:AACJV3JHV:2:1402:14880:45774/1‑138 (MQ=60)

                                                                                      GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggggggctgcgccgccccaaagggacacaatgcccctcccacaacccaccaccacgccctgcgccacacatactaaccaacgtcaggtt                                                                  >  VH01111:79:AACJV3JHV:2:2402:77973:24570/1‑62 (MQ=60)

                                                                                      GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTTCGCCTCACATCCAAACCAAGGTCAGGTT                                                                  >  VH01111:79:AACJV3JHV:1:1511:44911:21352/1‑153 (MQ=60)

                                                                                        CTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAACGTCAGGTTGC                                                                >  VH01111:79:AACJV3JHV:1:1610:60230:52381/1‑153 (MQ=60)

                                                                                               CGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggggggggcgcggcccgatacgggcaacacgccccgcccccaaaaaaacaacccaaaaccaacaaaaaactaaacaccccccccacacgcggtgtgt                                                         >  VH01111:79:AACJV3JHV:2:1514:67350:49465/1‑53 (MQ=60)

                                                                                               CGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggcggcggcgcccccccatcgcgccacaacggcggggccccaaactaacaaaccaaaaccaacacaacaacaaaccacccccccgcccggggggtgt                                                         >  VH01111:79:AACJV3JHV:2:1514:67482:49409/1‑53 (MQ=60)

                                                                                                     AGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCACCCCAAAAGCGACACAACGCCGCTCCCccaaacaaacaatccaaaatcaaaaaaaaaagaaaccaccccacctcaggtggtgtgtttttgg                                                   >  VH01111:79:AACJV3JHV:2:1105:28722:36705/1‑89 (MQ=56)

                                                                                                                   GCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggggggtggggcgcccgatcgcgccacaaccccccgcccccaccacaccaccacgcccaccgccccacaacataaaaaatctcatgttttagataagcggctccgcccaccccttta                                     >  VH01111:79:AACJV3JHV:2:2212:40272:46171/1‑33 (MQ=60)

                                                                                                                   GCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggggggtggggcgcccgataccgccacaatgccgcgcccccaacccaccaccccgccccgcgccgcacaaacaaaacaacgtgaggtttcacttacgcgtcacgccccacccctttc                                     >  VH01111:79:AACJV3JHV:2:1112:6586:30515/1‑33 (MQ=60)

                                                                                                                   GCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGTAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCC                                     >  VH01111:79:AACJV3JHV:1:2204:57788:24949/1‑153 (MQ=60)

                                                                                                                   GCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCtgtgtcttataaacatatacgagcccacgataatggatgtgttttgtggttttgcgtgtt                                     >  VH01111:79:AACJV3JHV:1:2208:29858:6793/1‑93 (MQ=60)

                                                                                                                   GCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAACGTCAGGGTGCAGATCCGCGTCACGCACCAGACATTCC                                     >  VH01111:79:AACJV3JHV:1:1408:14331:21333/1‑153 (MQ=60)

                                                                                                                   GCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTACAGACCACCAGGCCtgtctcttataaacatatacgagcccaccagactgcatgagttatctggtattgcgtctt                                     >  VH01111:79:AACJV3JHV:1:2208:50062:6698/1‑93 (MQ=60)

                                                                                                                   GCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAACGCCGCTCCCACACCAGACCACCAGGCCCATCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCC                                     >  VH01111:79:AACJV3JHV:1:2610:12835:32900/1‑153 (MQ=60)

                                                                                                                         GGCTTTCATTGCCACTGCATGAACCGCCGGGGGTGCTGGGCCGCCCTATAGCGACACAATGCCGCTCCCACTCCACAACACCAGGCCCTGCGCCacacaaactaaacacattctattataaaaaaataccaccccaccagacagcatgtgttt                               >  VH01111:79:AACJV3JHV:2:1403:74091:12889/1‑94 (MQ=44)

                                                                                                                         GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGGTGCGCCGCCCTATCGCGTCACAAGGCCGCTCCCACTACAGAACACCACGCCCTGGGCCTCACAAACAAAACAACGTCAGGTTGCAGATCCGCGTCACCCACCAGCCTTTACAATGGG                               >  VH01111:79:AACJV3JHV:2:1601:58659:22298/1‑153 (MQ=60)

                                                                                                                         GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCaatcccctcccaaagccccacccacaacaaaccaccacgcccagcgcctaaaaaacaaaacaacgtaaagttgcacaaacgcctcaagcaccagcctatcaaattcg                               >  VH01111:79:AACJV3JHV:2:1104:33626:45736/1‑46 (MQ=47)

                                                                                                                         GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCCCAGCCCTATCCCCACACAACGCCGCGCCCACTCCAGACCACCACGCCCTGCGCCTCACATCCAAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCG                               >  VH01111:79:AACJV3JHV:1:1414:66536:11583/1‑153 (MQ=60)

                                                                                                                                                  GG‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTC  >  VH01111:79:AACJV3JHV:2:1112:53622:5600/1‑153 (MQ=60)

TTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTC  >  AE015451_phaM1/5681268‑5681566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`