Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F13 I0 R1
|
213 |
60.4 |
2554362 |
98.6% |
2518600 |
147.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
3,791,727 |
T→G |
36.9% |
S531R (AGC→CGC) |
PP_3354 ← |
acyl‑CoA dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 3,791,727 | 0 | T | G | 36.9%
| 52.2
/ 8.5
| 37 | S531R (AGC→CGC) | PP_3354 | acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (17/6); new base G (2/12); total (19/18) |
| Fisher's exact test for biased strand distribution p-value = 6.29e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACGGCGTGGCGGCCGCGTCGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTCGCACGCAC > NC_002947_CJ‑RC/3791588‑3791864
|
aaCGGCGTGGCGGCCGCGTCGATGGCCGCCCCCAGGCGGGCGCGGTGGGGCGGCAGGTGGCCGCGGGAGAACCCAACGCGTTGCTGGGTTTACCGGGGCTGCGGCGGCCCCCCAGGCCCCGCCGGGCTTCAGCGCGCGCGGGGGGcccc > 6:202639/1‑148 (MQ=255)
aaCGGCGTGGCGGCCGCGTCGATGGCCGCCACCAGCCGGTCGAGGTGGGGGAGCAGGTAGCCGAGGAAGAACCCAACGCTTTACAGCTTTTACCGGCGCTGCGCCCCCCCCCCAGGCAACCCCGGGATGGACCCCCCCCGGCGGGcccc > 6:241522/1‑148 (MQ=255)
gggggggcggcCGGGGCGTTGGCCGCCACCTGCCGGTCGTGGTCGGGCAGCCGGTAGCCGAGGAACAACCCAACGCTTTCCTGCTTTTCCCGGCGCGGCGCCGCCCCCACTGGCAACGCCTGGAGGCAGCGCGCGCGGCGGGCCCAGGc < 8:133438/144‑1 (MQ=255)
gggcggcCGCGGCGATGGCCCCCACCTGCCGGTCGAGGGCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGTATGCAGCGCGCGCTGCGGGCCCAGGCGAAg < 2:256569/148‑1 (MQ=255)
ggcCGCGTCGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCt < 2:93488/149‑1 (MQ=255)
cgcgTCGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTg > 1:221905/1‑147 (MQ=255)
gTCGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTgcagca > 7:261902/1‑149 (MQ=255)
gTCGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTgcagca > 5:156814/1‑149 (MQ=255)
aTGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGt > 7:160713/1‑149 (MQ=255)
gTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAgg > 6:307187/1‑148 (MQ=255)
ggTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCg > 3:181887/1‑149 (MQ=255)
gTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGc < 2:302331/149‑1 (MQ=255)
gcGGGCCGCAGTTAGCCTAGTAACAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGAGGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGcc < 3:205446/148‑1 (MQ=255)
ttAGCCTAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCGGCGCCGCCCCCACTGGCAACGCCTGGATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGt < 1:209662/148‑1 (MQ=255)
aacaaCCCAACGCTTTCCTGCTTTTCCCGGCGGTGCGCCGCCCCCACTGGCAACGCCTGGATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTc < 8:282480/146‑1 (MQ=255)
cccaCCCAACGCTGTCCTGCTTAGCCCGGCGCGGCGCCGCCCCCCCTGGCACCGCCTGGCTGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCg < 7:13383/146‑1 (MQ=255)
agaaCCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCg < 1:238983/149‑1 (MQ=255)
gaaCCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCgg > 2:73056/1‑149 (MQ=255)
gaaCCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTCGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCCGTACGGAAATTCgg > 5:2765/1‑149 (MQ=255)
caaCCCACCGCTTTCCTGCTTTTCCCGGCGCGGCGCCGCCCCCACTGGCAACGCCTGGATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCgg < 5:250327/148‑1 (MQ=255)
cAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCg > 5:152052/1‑149 (MQ=255)
aaCGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTAGCCTGCGGCGCGGGTCGGAAAATCGGGATCGc > 6:186437/1‑149 (MQ=255)
tttCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCcgcg > 7:301391/1‑149 (MQ=255)
cTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTc > 7:287575/1‑148 (MQ=255)
cTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATc < 7:159634/148‑1 (MQ=255)
cTTTTCCCGGCGCTGCGCCGCCCCCACTGCCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATc < 2:58977/148‑1 (MQ=255)
ttCCCGGGGCTGCGCCGCCCCCTCTCGCAAGGGTTGTATGCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCg < 1:152695/146‑1 (MQ=255)
ttCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGa > 1:235573/1‑147 (MQ=255)
ttCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATCCAGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGa < 1:120623/147‑1 (MQ=255)
ttCCCGGCGCTGCGCCGCCCCCACTGCCACCGCCTGGATCCCGCGCGCGCGGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGa < 5:241522/147‑1 (MQ=255)
cGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCAc < 8:160713/149‑1 (MQ=255)
gcTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCCGCACCGTTGCGCACAACTGCAGGAAGTCCCCGGCGGCGCGGGACGGAAATTAGGGCTAGCCCGCGGTCCTCGACAGCCCCTTg > 1:174857/1‑149 (MQ=255)
tGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCCCGGTCATAGACAGCACCTTGGc > 3:258340/1‑149 (MQ=255)
ccACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGcc > 2:104498/1‑149 (MQ=255)
ccACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGcc > 7:257915/1‑149 (MQ=255)
cTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTcgc < 4:181887/149‑1 (MQ=255)
gCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTCgcacgcac < 5:185925/149‑1 (MQ=255)
|
AACGGCGTGGCGGCCGCGTCGATGGCCGCCACCTGCCGGTCGAGGTCGGGCAGCAGGTAGCCGAGGAAGAACCCAACGCTTTCCTGCTTTTCCCGGCGCTGCGCCGCCCCCACTGGCAACGCCTGGATGCAGCGCGCGCTGCGGGCCCAGGCGAAGGCCTGCAGCACCGTTGCGCACAACTGCAGGAAGTCGCCTGCGGCGCGGTACGGAAATTCGGGATCGCCCGCGGTCATCGACAGCACCTTGGCCAGCGCTGCCTCCAGCGTGTCGCACGCAC > NC_002947_CJ‑RC/3791588‑3791864
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
