Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F13 I0 R1
|
213 |
60.4 |
2554362 |
98.6% |
2518600 |
147.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
4,586,163:1 |
+C |
100% |
intergenic (+139/+76) |
PP_4061 → / ← PP_4063 |
hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 4,586,163 | 1 | . | C | 83.3%
| 47.7
/ 5.1
| 18 | intergenic (+139/+76) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (7/8); total (8/10) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG > NC_002947_CJ‑RC/4586035‑4586305
|
cGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATgc < 1:221624/149‑1 (MQ=39)
ccGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGgcgc < 3:96793/149‑1 (MQ=39)
cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTTAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt < 3:15584/149‑1 (MQ=255)
cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGc < 5:230333/144‑1 (MQ=38)
cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg < 1:250798/147‑1 (MQ=38)
ctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAc < 2:179441/147‑1 (MQ=38)
tCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTgg < 8:207016/86‑1 (MQ=21)
tCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTgg > 7:207016/1‑86 (MQ=21)
gggTAAACCCGCTCCTACCAGGGCC‑C‑GTGCCGGCCTTGAAAGCGGCGCTGCCCCGGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACGGATCTCACGCATg > 3:210430/2‑149 (MQ=38)
ccGCTCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACtt > 2:196262/1‑149 (MQ=255)
tCCTACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTg > 3:118310/1‑148 (MQ=255)
cTACCAGGG‑A‑CCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAagaggccggcccgatcaatgcaagatcttcaccttgccaagcacccggtcatcctcaacctttcaatggagcagcgcagatcagc < 1:14159‑M2/149‑86 (MQ=255)
tACCAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCAcc > 2:4342/1‑149 (MQ=255)
tACCAGGG‑A‑CCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAagaggccggcccgatcaatgcaagatcttcaccttgccaagcacccggtcatcctcaacctttcaatggagcagcgcagatcagct < 6:245232‑M2/149‑87 (MQ=255)
cAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTg > 7:174265/1‑149 (MQ=255)
cAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTg > 7:28239/1‑149 (MQ=255)
cAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTg < 1:196262/149‑1 (MQ=255)
cAGGG‑ACC‑GTGCCGGCCTTGAATGCGGCGCTGCCCATGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGAACATCAACCCGCAGATGCAGCCGCAATCTACGCCACACTGATCTCACGCATGCGGAACTTCTGCACATTg > 5:285182/1‑149 (MQ=39)
|
CGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑C‑GTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTG > NC_002947_CJ‑RC/4586035‑4586305
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
