Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F13 I0 R1 213 60.4 2554362 98.6% 2518600 147.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947_CJ‑RC 5,991,720 T→C 13.1% intergenic (+199/‑347) kefB‑III → / → PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC5,991,7200TC13.1% 95.7 / 6.0 38intergenic (+199/‑347)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (18/15);  new base C (2/3);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACT  >  NC_002947_CJ‑RC/5991573‑5991867
                                                                                                                                                   |                                                                                                                                                   
gcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGAtt                                                                                                                                                    <  7:137822‑M2/142‑1 (MQ=255)
 cccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTg                                                                                                                                                   <  1:289569‑M2/143‑1 (MQ=255)
 cccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTg                                                                                                                                                   <  2:139243‑M2/143‑1 (MQ=255)
    gcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGcc                                                                                                                                                <  2:229569‑M2/146‑1 (MQ=255)
               cgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac                                                                                                                                      <  5:276453/148‑1 (MQ=255)
               cgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaaac                                                                                                                                      <  2:55570/148‑1 (MQ=255)
                gcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACg                                                                                                                                     >  5:288077/1‑148 (MQ=255)
                gccggTAAACCCCCTCCTACCTTTGTTTGGGGCCAGTTATTCCTGTGGGACGCCCACGCGACCCCCCAGGCGCCCCCCACCAAATCGCGGCCTACAAACAAGGCGGTCCCCCGCGCCTCGCACACGAGCGATTCGCCACAAACCAACg                                                                                                                                     >  8:64067/4‑148 (MQ=255)
                  gggTAAACCCGCTCCTTCGTTTGTTTCGGGACAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACTAGGCGGTCGCGCGCGCCTGGCACTGGAGGGATTGGCCAGAAACAAACGTAg                                                                                                                                  <  4:179068/149‑1 (MQ=255)
                   ggTAAACCCGCTACAACCTTTGTTTAGGGCCAGTTATTGCTGTGGGCCGCGCACGCCAGCCCCCTGGCCCCCGCCGGGATATCGCGGCGTACAAACAAGGCGGTCGCGCGCGCCGGGCACAGGGGGGATTGGCCAGAAACAAACCTAgg                                                                                                                                 >  4:105855/1‑149 (MQ=21)
                        aCCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCgg                                                                                                                            <  8:287285/149‑1 (MQ=255)
                             cTCCTACGTTTGTTTCGGGCCAGTTATTCCTGGGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGCCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTa                                                                                                                       <  1:199809/149‑1 (MQ=255)
                                cTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCGTGGGGCATGTGTCGATATGGCGTCGTTCAAACAAGGCGGTCGCGCGCGAAGGGTGGAGGAGGGCCTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCcg                                                                                                                   >  5:303129/1‑149 (MQ=2)
                                     tttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcg                                                                                                                 <  8:37755‑M2/147‑7 (MQ=255)
                                     tttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcg                                                                                                                 <  2:12809‑M2/147‑7 (MQ=255)
                                          ttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgcc                                                                                                          <  7:303733‑M2/149‑14 (MQ=255)
                                                   gTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcg                                                                                                 <  7:271521‑M2/149‑23 (MQ=255)
                                                     tATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTGACGATATGGCGTCGGAAAAACAAGGCGGTCGGGCGCGACTGGTGCAGGAGGGACTGGGC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGgcggcg                                                                                              >  6:69795/1‑149 (MQ=12)
                                                          cTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctc                                                                                           <  8:39686‑M2/148‑29 (MQ=255)
                                                                                cccTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCGCGATCTCATGGGCGCCCAAAAAc                                                                     >  7:290249/1‑148 (MQ=255)
                                                                                   tGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATc                                                                 >  4:53034/1‑149 (MQ=255)
                                                                                   tGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATc                                                                 >  6:264374/1‑149 (MQ=255)
                                                                                           gCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCa                                                         >  5:70978/1‑149 (MQ=255)
                                                                                                gATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctca                                                    <  2:284290‑M2/148‑68 (MQ=255)
                                                                                                        gtcgtACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCAt                                             >  5:296383/1‑148 (MQ=255)
                                                                                                            tACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTg                                         >  5:123673/1‑148 (MQ=255)
                                                                                                            tACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTg                                         >  3:150449/1‑148 (MQ=255)
                                                                                                               aaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAt                                     >  4:230110/1‑149 (MQ=255)
                                                                                                               aaacaaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAt                                     >  3:228497/1‑149 (MQ=255)
                                                                                                                 acaaGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtcca                                  <  7:191029‑M2/149‑86 (MQ=255)
                                                                                                                   aaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTcc                                 >  3:136815/1‑149 (MQ=255)
                                                                                                                   aaGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCAGCAAtttac                                 >  4:235573/1‑147 (MQ=255)
                                                                                                                              gcgcgcCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAACCGCCGCGCCGGCCGCGCTAGATGTCAATGGCGCCCAAAAACTATAGCCAGGCAACTGGAAGCCATACTGCAAATTGCTGACGATCAcc                      >  3:177440/1‑149 (MQ=25)
                                                                                                                                  gcCCGGCACAGGAGGGTTTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgc                  >  6:248367/1‑149 (MQ=255)
                                                                                                                                   ccTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgcc                 <  7:39686/149‑1 (MQ=255)
                                                                                                                                    cTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgccg                >  7:281327/1‑149 (MQ=255)
                                                                                                                                     tGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgccgc               <  3:53034/149‑1 (MQ=255)
                                                                                                                                          caGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccagtcaaaattccaacgccgccccct          <  7:97357‑M2/148‑110 (MQ=255)
                                                                                                                                               ataattgGCCCGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctcaaaa     >  2:33225‑M2/4‑35 (MQ=255)
                                                                                                                                                  aTTGGCCAGAAACAAATGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACt  >  6:116166/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACT  >  NC_002947_CJ‑RC/5991573‑5991867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A