Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F13 I1 R1
|
59 |
72.5 |
3655346 |
98.4% |
3596860 |
137.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
1,499,508 |
+C |
intergenic (+85/‑79) |
trpS → / → zapE |
tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 1,499,506 | 1 | . | C | 94.4%
| 53.2
/ ‑2.8
| 18 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (11/6); total (12/6) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 |
CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG > NC_002947_CJ‑RC/1499360‑1499649
|
cGCTGACGCCCGTGATGCGGCCATTCAGAGCCTGAAGGAAGGCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAc‑g‑c < 4:181102/149‑1 (MQ=25)
gcggAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAc‑g‑cc < 5:281157/146‑1 (MQ=37)
gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACAC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 7:272131/1‑149 (MQ=25)
cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 5:376560/1‑148 (MQ=37)
ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 8:442517/1‑147 (MQ=37)
ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 7:356960/1‑147 (MQ=37)
ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATAAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 5:382995/1‑147 (MQ=25)
aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATAAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 6:382995/149‑1 (MQ=37)
cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 1:1129/1‑147 (MQ=37)
ccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 7:442517/149‑1 (MQ=37)
cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 7:352543/1‑147 (MQ=37)
ggTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 2:85359/1‑120 (MQ=25)
ggTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:85359/120‑1 (MQ=25)
ggcaccGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc > 7:275586/6‑138 (MQ=38)
ggcaccGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 8:275586/133‑1 (MQ=38)
ccGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc < 2:1129/147‑1 (MQ=39)
gtgttcgcGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa > 7:382525/8‑149 (MQ=255)
gggCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt > 4:313804/1‑99 (MQ=255)
gggCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt < 3:313804/99‑1 (MQ=255)
gCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcggcg > 7:355747/1‑149 (MQ=255)
|
CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG > NC_002947_CJ‑RC/1499360‑1499649
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGC > NC_002947_CJ‑RC/1499393‑1499650
|
gggggggtttcccgtgggggcctcctcctccctcccccccggggggggggggggggcccccccaaccccggggcgggggggccgccccgggttggggggggggggggggccccccccCCGCTCCCACAGACTGATTGCCATTAAGCGGGCC < NB501016:282:H2V7GAFX2:4:21609:12987:16950/34‑1 (MQ=60)
ccacctttgcccccccctcccgtctggggccccaattggggggggggggggggcccccaaaaccggggcggggggttcgggccccggggttgggggggttggggggcccccccCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATC < NB501016:282:H2V7GAFX2:3:11608:20070:17245/38‑1 (MQ=60)
cccccccccccccctcccggggcccccactcggggggggggggggcgccccccaaacccggcccggggggccccgccccggggttggggggggggggggccccccccCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACA < NB501016:282:H2V7GAFX2:4:21605:19310:16482/44‑1 (MQ=60)
ccccccgccccccccccccaccgggggggggggggggccccccccaacccgcccccgggggcccccccccgggtgggggggggggggccccccccccccCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGC < NB501016:282:H2V7GAFX2:2:21211:5840:2962/52‑1 (MQ=60)
tgttcggggccccaactggggggggggggggggccccctaaatcggggggggggtctgccgcccggggtgtgggggtgtgggcgggcccccccCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGT < NB501016:282:H2V7GAFX2:3:11506:16948:10785/56‑1 (MQ=60)
gggggggggggggggccgccccccacccccggcgggggggccccccccgcgggggggggggggggcgcccccccccccCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTA < NB501016:282:H2V7GAFX2:2:11111:16063:4115/73‑1 (MQ=60)
GGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACAC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTC > NB501016:282:H2V7GAFX2:4:21406:9027:12040/1‑151 (MQ=60)
cgcccccccgcccgggggggggggttggggggcgggggggggggggggcccccccccaacgcCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCC < NB501016:282:H2V7GAFX2:2:21306:11468:4711/89‑1 (MQ=60)
GCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTT > NB501016:282:H2V7GAFX2:3:21511:11509:16774/1‑150 (MQ=60)
CCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTT > NB501016:282:H2V7GAFX2:4:21506:10703:16691/1‑149 (MQ=60)
CCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATAAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTT > NB501016:282:H2V7GAFX2:3:21512:20089:17296/1‑149 (MQ=60)
AACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCT > NB501016:282:H2V7GAFX2:1:11101:13911:5033/1‑150 (MQ=60)
GCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCA < NB501016:282:H2V7GAFX2:4:21607:21443:18418/151‑1 (MQ=60)
CCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGA > NB501016:282:H2V7GAFX2:4:21506:22802:4092/1‑149 (MQ=60)
GCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATG < NB501016:282:H2V7GAFX2:1:11202:9188:4425/124‑1 (MQ=60)
TGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCA > NB501016:282:H2V7GAFX2:4:21407:14938:2174/1‑140 (MQ=60)
ggggggggggggggggcgcccccccccccCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTC < NB501016:282:H2V7GAFX2:2:21306:1324:15195/120‑1 (MQ=60)
CGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAA < NB501016:282:H2V7GAFX2:3:21510:8155:13794/134‑1 (MQ=60)
ggtgttcgCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAT > NB501016:282:H2V7GAFX2:4:21510:14787:13279/9‑151 (MQ=60)
gggcggcaccccCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG < NB501016:282:H2V7GAFX2:1:21106:2426:10940/138‑1 (MQ=60)
CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA < NB501016:282:H2V7GAFX2:2:21205:20844:4648/101‑1 (MQ=60)
GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGC > NB501016:282:H2V7GAFX2:4:21506:15339:13253/1‑151 (MQ=60)
GGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACG > NB501016:282:H2V7GAFX2:1:21111:22646:13791/1‑104 (MQ=60)
CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGC > NB501016:282:H2V7GAFX2:2:21203:8550:10540/1‑149 (MQ=60)
|
GAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGC > NC_002947_CJ‑RC/1499393‑1499650
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
