Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F13 I0 R1
|
203 |
85.6 |
3649704 |
98.8% |
3605907 |
146.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
3,845,703:1 |
+G |
100% |
intergenic (‑70/+28) |
PP_3394 ← / ← PP_3395 |
3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 3,845,703 | 1 | . | G | 100.0%
| 72.4
/ NA
| 21 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (11/10); total (11/10) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATG > NC_002947_CJ‑RC/3845556‑3845821
|
ggCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTcgc < 5:421579/148‑1 (MQ=255)
gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTcgc‑g < 4:293069/148‑1 (MQ=255)
ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑ggg < 1:441228/149‑2 (MQ=255)
ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑ggg < 7:330762/149‑2 (MQ=255)
tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggc < 2:392220/149‑3 (MQ=255)
tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTATGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggc > 4:301579/1‑147 (MQ=255)
ctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggc < 5:170713/147‑3 (MQ=255)
tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGAt < 6:237984/148‑1 (MQ=255)
cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATc < 7:268897/149‑1 (MQ=255)
aTGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCG‑CAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGt < 2:155455/149‑1 (MQ=255)
tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCa < 7:337324/148‑1 (MQ=255)
aCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCAACGTCAGcc > 6:133544/1‑149 (MQ=255)
cTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCa < 6:286621/149‑1 (MQ=255)
gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATACATAGTGAGCCACGa > 7:100503/1‑149 (MQ=255)
cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGAt < 4:192379/149‑1 (MQ=255)
cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGAt < 6:204275/149‑1 (MQ=255)
gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCAGATCCATAGTCGGGCACgcagga > 7:106591/1‑144 (MQ=255)
ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGAt < 6:455719/149‑1 (MQ=255)
ataGCCATCGCGCAGGCCGACTTCATTGTTTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGTTGGGTTGCGCCAGACTAAGTGCTGGTTGACGGGCAGATTAGACGTGTTTCGCGGGCTGATCGATGGTCATCCACGATGGATCa < 6:245428/144‑1 (MQ=255)
aaGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATCCATCGTCAGCCACGATGGATCAGccc < 5:168865/149‑1 (MQ=255)
aTCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCAGATACATCGTCAGCCACCATGGTTCAACCCCCGcc > 1:6794/1‑149 (MQ=255)
gttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATACAAAGTGCCCCAACATGGAACACCCCCCCCCCCGCCCAGCCGGTGCACCCCGCaa > 6:149923/1‑148 (MQ=255)
ctctctGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGGTGATCCATAATCAAGCCCGGTTGTTCACCCCGCCCCCGTCCATGCCGGTTCACCCCACAACAAGGAGTAAAGCAAGcccc > 2:12288/1‑148 (MQ=255)
gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATACAAAGGCAGCCACCATTGGTAAACCCCCCCCCAGCCACGCCCGTGCACCCCGCAAAACGTGGTCAAACACGCCCCCACCCCACACCGAGAGCgtgt > 4:220326/1‑149 (MQ=255)
aGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCAGATACATAGTCAGCCCCGATGGATAAGCCCCCCCCCAGCCACGCCGGTGCCCGCAGCCACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGcc > 6:123663/1‑149 (MQ=255)
agagCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATACATAGTCAGCCCCGAAGGATCCGCCCCCCCCCAGGCCGGCCCGGGGCCGCACCAACAGGTGGTCAACCACGCGCCGCGCCCCCAGCGCCAGCGGGTGCCCGTcgc > 8:355682/1‑149 (MQ=255)
gCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCTGATACATACTCAGCCACCATGGATAAGCCCCCGCCCAGCCAGGCCGGTGCACGCACCAACAGGTGGTCAACCACGCGCCCAGCCCACAGCGAGAGCGTGTGCCCGTCgcgc > 2:277552/1‑148 (MQ=255)
tGGATGACGGGCAGAAAAGACGTGTTTCGCGGGCAGATACAAAGGCAGCCACGCAGGGTAAACCCCCCCCCAGCCCCGCCCGGGGACCCCGCAAAAAGTGGTGAAACAAGCGCCCCGCCCCCAGCGAGAGCGTGTGCCGGgcgcgccag > 6:118634/1‑146 (MQ=255)
|
GGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATG > NC_002947_CJ‑RC/3845556‑3845821
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
