Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F13 I0 R1 203 85.6 3649704 98.8% 3605907 146.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947_CJ‑RC 4,551,577 T→G 20.2% V280G (GTG→GGG)  pydA → NADP‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC4,551,5770TG20.2% 145.4 / 5.8 66V280G (GTG→GGG) pydANADP‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand):  ref base T (11/41);  new base G (11/3);  total (22/44)
Fisher's exact test for biased strand distribution p-value = 1.29e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGAAATAC  >  NC_002947_CJ‑RC/4551435‑4551725
                                                                                                                                              |                                                                                                                                                      
tCGGCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGgt                                                                                                                                                  >  7:339913/1‑149 (MQ=255)
 gggCAGTGCAGCCGATTGCCTTGAACATGGTGTCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGGCGATTTGTGGGATTGGCGGCATCGGCAACTGGCGTGAGGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGgt                                                                                                                                                  <  5:434170/147‑1 (MQ=255)
 cGGCAGTGAAGCCGATTGCTTTGAACTTGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGgt                                                                                                                                                  <  4:252217/148‑1 (MQ=255)
  ggCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCATCGCCGCCGTGCacacct                                                                                                                                                <  4:250985/149‑6 (MQ=255)
  ggCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGgtgt                                                                                                                                                <  3:271027/149‑1 (MQ=255)
         aaGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCAcggc                                                                                                                                         >  5:396064/1‑149 (MQ=255)
                ttGCCTTGAACTTGGTGGGGCAACGCCCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGa                                                                                                                                    <  1:198910/147‑1 (MQ=255)
                ttGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGAtg                                                                                                                                  >  6:99410/1‑149 (MQ=255)
                   ccTTGAACATGGTGGCGGAAATCGCCCGCGACCAGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCATGCAGGTGTGCACGGCGGCGGggctg                                                                                                                               >  4:373757/1‑149 (MQ=255)
                    cTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCACCGAGTTCATGGCCTTGGGCAGTGGCGCCCGGCGGGGGTGGACGGGGGCGctgctg                                                                                                                               >  7:44785/1‑148 (MQ=255)
                           aTGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCtt                                                                                                                       <  1:285144/149‑1 (MQ=255)
                              gtggCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTcc                                                                                                                     <  2:280974/148‑1 (MQ=255)
                              gtggCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTcc                                                                                                                     <  6:166365/148‑1 (MQ=255)
                                 gCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCCGGCACGTGTGGACGGCGGCGCAGCTGCAAGGcgtgcgga                                                                                                                  >  4:50754/1‑141 (MQ=255)
                                      aaTCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTAtcgtc                                                                                                             <  4:301913/148‑1 (MQ=255)
                                       aTCGCCCGCGACCCGTTAACGCGGGGCTTGCCGATTTGTGGCTTTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGa                                                                                                           <  8:280307/149‑1 (MQ=255)
                                       aTCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGa                                                                                                           <  7:111519/149‑1 (MQ=255)
                                       aTCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGa                                                                                                           <  8:339913/149‑1 (MQ=255)
                                           cccGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCCGGTGTGGCCGGCGGCGCTGCTGGATGGGGTGCGGATGGTCGAgggc                                                                                                       >  1:29517/1‑147 (MQ=255)
                                            ccGCGACCCGGAAACGCGGGGCTTGCTGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGGGCAGGTGTGGACGGCGGCGATGCTGCATGGCTTGCGGATCGTCGAGGGCa                                                                                                      >  5:212804/1‑149 (MQ=255)
                                               cgACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCCTCGAGGACAT‑Gc                                                                                                  <  8:147641/149‑1 (MQ=255)
                                                 aCCCGGAAACGCGGGGGTTGCCGATTTGTGGCATTGGCGGCATGGGAAACTGGCGTGACGCCGCCGAGTTCTTGGCCTTGGGCTGTGGGGCGGTGCAGGTGTGCACGGGGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAg                                                                                                <  8:177233/149‑1 (MQ=255)
                                                    cGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCTAGTTCATGGCCTTGGGCAGTGGCGCCGTGCTGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGAc                                                                                             <  6:316944/149‑1 (MQ=255)
                                                    cGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTATGCACGGCGGCGATGCTGCATGGCGTCCGTATAGTCGAGGACAT‑GCAGGAc                                                                                             >  5:209826/1‑149 (MQ=255)
                                                      gAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGGGGAGGTGTGGACGGCGGGGCTGCTGGATGGGGTGCGTTTAGTGGAGGGCCT‑GGAGGGCgg                                                                                           >  5:179775/1‑149 (MQ=255)
                                                        atcgcgGGGCTTGCCGATTTGTGGCTTTGGCGGCATCGGCAACTGGGGTGACGCCGCCGAGTTCATGGCGTTGGGCTGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGcc                                                                                         <  4:183039/147‑1 (MQ=255)
                                                        aaCGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGcc                                                                                         <  7:466861/149‑1 (MQ=255)
                                                        aaCGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGGGCAGGTGTGGCCGGCGGGGGTGGTGGATGGGTTGCGTTTCGTCGAGGGCAT‑GCAGGGCGGcc                                                                                         >  5:207393/1‑149 (MQ=255)
                                                              gggCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCAc                                                                                   <  6:218268/149‑1 (MQ=255)
                                                                 cTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCt                                                                                <  6:316002/149‑1 (MQ=255)
                                                                 cTTGCAGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGACTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGAGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCt                                                                                <  6:203931/149‑1 (MQ=255)
                                                                    gCCGATTTGTGGCATTGGCGGCAGCGGCAACGGGCGGGACGCCGCCGAGTTCTTGGCCTTGGGCAGGGGCGCCGGGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCtgg                                                                              <  3:80413/148‑1 (MQ=255)
                                                                     ccGTTTTGTGGCTTTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCTGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCtggat                                                                            <  2:398397/149‑1 (MQ=255)
                                                                     ccGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCtggat                                                                            <  7:45462/149‑1 (MQ=255)
                                                                          ttGTGGCATTGGCGGCATCGGAAACTGGCGTGACGCCGCCGAGTTCATGGCATTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGAc                                                                        <  8:467276/148‑1 (MQ=255)
                                                                           tgtgGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGAcc                                                                       <  6:301687/148‑1 (MQ=255)
                                                                            gtgGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCa                                                                      <  4:120939/148‑1 (MQ=255)
                                                                            gtgGCATTGGCGGCATCGGCAACTGGCGTGACGCAGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTGCGTATAGTCGAGGACAA‑GCAGGACGGCCTGGCACGCTGGATGGACCAg                                                                     >  6:419887/1‑149 (MQ=255)
                                                                             tgGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCCTGCAGGTGTGCACCGCGGCGCTGCAGCAAGGCGTGCGGATAGGCGAGGACAA‑GCAGGACCGCCAGGCACCCAGGATGGACCac                                                                     >  6:323707/1‑147 (MQ=255)
                                                                               gCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCAt                                                                  <  8:172871/149‑1 (MQ=255)
                                                                                       ggcATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGGGCCGGGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGc                                                          <  5:249904/149‑1 (MQ=255)
                                                                                       ggcATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGc                                                          <  7:446923/149‑1 (MQ=255)
                                                                                          aTCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCCGGGAGGTGTGGACGGCGGGGCTGCTGGATGGGGTACGTATAGTGGAGGGCAA‑TCAGGCCCGCCCGGCCCGCGGGGTGGGCCACCCAGGGCAAGCCa                                                        >  4:432158/1‑148 (MQ=255)
                                                                                             ggCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTg                                                     <  8:121336/148‑1 (MQ=255)
                                                                                              gcatgtGGCGTGAGGCCGCCGAGTTCTTGGCCTTGGGCTGTGGCGCGGTGCTGGGGTGCAGGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGAGCAGCATGGGCATGCCACTGTc                                                   <  1:433765/144‑1 (MQ=255)
                                                                                              gCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGGGCAGGTGTGCACGGCGGCGATGCTGCATGGCATCCGTATCGTCTCGGACAA‑GCAGGACGGCCGGGCACCCAGGATGGACCCGCAAGGGCATGCCACAGTc                                                   >  2:312955/1‑149 (MQ=255)
                                                                                                  cTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAg                                              <  3:50754/149‑1 (MQ=255)
                                                                                                  cTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAg                                              <  6:253314/149‑1 (MQ=255)
                                                                                                     gCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGc                                             <  8:83008/147‑1 (MQ=255)
                                                                                                       gTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCCGGGAGGTGTTCACGGCGGCGCTTCTGCATGGGGTGCCTATAGTAGAGGACATGGAAGGAC‑GCCAGGCCCCCAGGATGGGACACCAAGGGGATTCCACAGTAG‑aaaccttg                                         >  8:25351/1‑143 (MQ=255)
                                                                                                           cgccgcCGAGTTCATGGCCTTGGGCTGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCcgcg                                     <  2:288828/149‑1 (MQ=255)
                                                                                                            gccgccGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCgg                                    <  2:158359/149‑1 (MQ=255)
                                                                                                            gccgccGAGTTCATGGCCTTGGGCAGTGGCGCCGGGCAGGTGTGCACCGCGGCGCTGCTGCATTGCGTGCGTATAGTCGAGGACAA‑GCAGGGCGGCCGGGCCCGCTGGAAGGGCCAGCAAGGGCATTCCACAGTAG‑AAGCGTTACGCgg                                    >  6:110262/1‑149 (MQ=255)
                                                                                                              cgccgAGTTCATGGCCTTGGGCAGTGGCGCCCGGCACGTGTGGACGGCGGGGCTGGGGCAAGGGGTTCGTTTGGTGGAGGCCAA‑TGAGGCGGGCCTGGCACGCGGGTAGGGCCAGCCTGGGCCAGCGACGGGAG‑AAGAGGTGCGCGGGc                                  >  2:52814/1‑149 (MQ=255)
                                                                                                                     ttCATGGCCTTGGGCAGTGGCGCCGGGCAGGTGTGCACCGCGGCGCTTCTGCAAGGCTTACGTATAGTGGAGGGCAT‑ACACGACGGCCAGGCAACCAGGATAGCACAACAATGGCCAGCCACAGTCGAAAGCG‑TACGCGGGCAGGccgg                           >  8:138665/1‑146 (MQ=255)
                                                                                                                       cATGGCCTTGGGCAGTGGCGCCGTGCAGGGGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTg                          <  3:99977/148‑1 (MQ=255)
                                                                                                                        aTGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCTGTgg                         <  6:398029/148‑1 (MQ=255)
                                                                                                                        aTGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTggg                        <  6:396064/149‑1 (MQ=255)
                                                                                                                        aTGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTggg                        <  1:441880/149‑1 (MQ=255)
                                                                                                                         tGGCCTTGGGCTGTGGCGCCGGGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTgggg                       <  3:387859/149‑1 (MQ=255)
                                                                                                                                      tGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACt          <  5:99410/149‑1 (MQ=255)
                                                                                                                                        gcgcCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTg         <  7:448801/148‑1 (MQ=255)
                                                                                                                                          gcCGTGCAGGTGTGCTGGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGaa      <  1:312955/149‑1 (MQ=255)
                                                                                                                                          gcCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGa       >  4:431419/1‑148 (MQ=255)
                                                                                                                                          gcCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGa       >  8:151645/1‑148 (MQ=255)
                                                                                                                                          gcCGTGCAGGTGTGCACGGCGGCAATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGa       >  7:211163/1‑148 (MQ=255)
                                                                                                                                              tGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGAAATAc  <  6:212804/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                      
TCGGCAGTGAAGCCGATTGCCTTGAACATGGTGGCGGAAATCGCCCGCGACCCGGAAACGCGGGGCTTGCCGATTTGTGGCATTGGCGGCATCGGCAACTGGCGTGACGCCGCCGAGTTCATGGCCTTGGGCAGTGGCGCCGTGCAGGTGTGCACGGCGGCGATGCTGCATGGCTTCCGTATCGTCGAGGACAT‑GCAGGACGGCCTGGCACGCTGGATGGACCAGCATGGGCATGCCACTGTCG‑AAGCGTTCCGCGGGCAGGCGGTGGGGCATACCACCGACTGGAAATAC  >  NC_002947_CJ‑RC/4551435‑4551725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A