Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F13 I0 R1 203 85.6 3649704 98.8% 3605907 146.1

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC  >  NC_002947_CJ‑RC/4586000‑4586212
                                                                                                                                               |                                                                       
CCAGGAGCCACCGGAGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCC                                                                  <  NB501016:282:H2V7GAFX2:4:11408:21169:13468/151‑1 (MQ=60)
 CAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCG                                                                 <  NB501016:282:H2V7GAFX2:2:11307:20932:3917/151‑1 (MQ=60)
   GGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT                                                               <  NB501016:282:H2V7GAFX2:1:11102:10286:7077/151‑1 (MQ=60)
   GGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCT                                                               <  NB501016:282:H2V7GAFX2:3:21408:12752:16017/151‑1 (MQ=60)
         ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                         <  NB501016:282:H2V7GAFX2:3:21405:16801:7065/151‑1 (MQ=60)
                       AAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGCATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGG                                           <  NB501016:282:H2V7GAFX2:3:11503:25639:10451/151‑1 (MQ=60)
                           GTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTT                                       <  NB501016:282:H2V7GAFX2:1:11206:25260:12205/151‑1 (MQ=60)
                           GTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTT                                       <  NB501016:282:H2V7GAFX2:3:11508:5191:2822/151‑1 (MQ=60)
                               ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATAGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGTCTTGAAT                                   <  NB501016:282:H2V7GAFX2:3:21504:10757:10029/151‑1 (MQ=60)
                                CCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                         <  NB501016:282:H2V7GAFX2:2:11111:25496:17023/128‑1 (MQ=60)
                                  ACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCG                                <  NB501016:282:H2V7GAFX2:1:21206:9312:1200/151‑1 (MQ=60)
                                        CCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTG                          <  NB501016:282:H2V7GAFX2:4:11403:18667:11851/151‑1 (MQ=60)
                                          GGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCC                        <  NB501016:282:H2V7GAFX2:3:21508:2786:2979/151‑1 (MQ=60)
                                                 ACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTA                 <  NB501016:282:H2V7GAFX2:1:21207:2780:13875/151‑1 (MQ=60)
                                                       GATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                  >  NB501016:282:H2V7GAFX2:2:11307:2505:17441/1‑144 (MQ=60)
                                                            GCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGgcagcgccgcattcaaggccggcacggtccctggtaggagcggg         <  NB501016:282:H2V7GAFX2:1:21203:11474:19707/148‑45 (MQ=60)
                                                              GCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAC    <  NB501016:282:H2V7GAFX2:2:11111:5371:2041/151‑1 (MQ=60)
                                                                         GAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                  >  NB501016:282:H2V7GAFX2:4:21612:17469:18060/1‑126 (MQ=60)
                                                                                                                                              GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC  <  NB501016:282:H2V7GAFX2:4:21508:3226:3105/73‑1 (MQ=60)
                                                                                                                                               GTAAACCCGCTACTACCAGGGACCGTGCCGGCCTTGAATGCGGCG                             >  NB501016:282:H2V7GAFX2:4:21407:25446:13150/1‑45 (MQ=60)
                                                                                                                                               |                                                                       
CCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC  >  NC_002947_CJ‑RC/4586000‑4586212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑