Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F25 I1 R1
|
60 |
73.8 |
3348830 |
98.7% |
3305295 |
145.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
1,126,647 |
+C |
intergenic (‑101/+48) |
tdcG‑II ← / ← gcvP‑I |
L‑serine dehydratase/glycine dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 1,126,645 | 1 | . | C | 100.0%
| 62.0
/ NA
| 18 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (11/7); total (11/7) |
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGC > NC_002947_CJ‑RC/1126498‑1126782
|
tGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑c < 4:311412/149‑1 (MQ=255)
gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑cc < 3:141609/148‑1 (MQ=255)
cttgtgGCCCTCCTGCCTGGTTGTTGGGTGGACATTGAGCCCTGTTGGCGGGGGTTTACCGGCTATTGGGTTGGTATTGCCACCATCTCATTGGCGGTTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAgg < 2:296075/146‑1 (MQ=255)
ccAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGATAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCt < 6:238262/148‑1 (MQ=255)
gTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGc > 5:220493/1‑149 (MQ=255)
cccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGCTGGCGGGCAGGGCTATCAGGAGTCCATATAGCTCACGATCGACGGGCACGCGCACACCAGGTTGCGGTCg > 4:34948/1‑149 (MQ=38)
ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 3:376073/1‑149 (MQ=255)
gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg < 6:94174/95‑1 (MQ=255)
gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg > 5:94174/1‑95 (MQ=255)
ggAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt > 7:130341/1‑149 (MQ=255)
tataGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt > 1:221540/1‑144 (MQ=255)
tataGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt < 2:221540/144‑1 (MQ=255)
cccacaaTCGTATTGGGGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc < 8:218123/147‑1 (MQ=255)
ccaccaTCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAgg < 4:190963/108‑1 (MQ=255)
ccaccaTCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAgg > 3:190963/1‑108 (MQ=255)
caccaTCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc > 1:395741/1‑149 (MQ=255)
ccaTCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCg > 4:393396/1‑149 (MQ=255)
tCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAcc < 4:376073/149‑1 (MQ=255)
aTTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACtggcg > 4:8666/1‑149 (MQ=255)
gcgGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGGCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc > 8:232965/1‑147 (MQ=255)
|
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGC > NC_002947_CJ‑RC/1126498‑1126782
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACTTGCCTT > NC_002947_CJ‑RC/1126498‑1126795
|
GGGAGGGGCCGATGCCTATGTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCTGGTTGTTGGGCGGACATTGAGCCCTGTCGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCC < NB501016:282:H2V7GAFX2:1:21211:20221:2886/151‑1 (MQ=60)
GGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCC < NB501016:282:H2V7GAFX2:2:11301:6496:11709/150‑1 (MQ=60)
cccactcgctcgtgtacctggccccccgcccgcgggggtgtgggtgccgccagaccgcgtggggggggtgtttccccctgtttgttcttttccaccacccccccgcccgcgcctaaccccCCGCTCCTACACAGAGCCCGCCACCCAAGCC < NB501016:282:H2V7GAFX2:2:11205:14680:16879/31‑1 (MQ=60)
ccgccacggcgccccccccgccgtggggggggggctcacttccccccgtgcggggggggtcccccccgcccggggggttcaacccccccccccccaggggggtaaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGG < NB501016:282:H2V7GAFX2:3:11404:25351:8315/42‑1 (MQ=60)
ccccgcggccggggttgttgggcccatttgcccccttggggggggggtttacccggttatgggggtgttaaccccccccgcgattcgggttaaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGG < NB501016:282:H2V7GAFX2:1:21209:17248:12648/54‑1 (MQ=60)
ccccccgccgtggtttggggggccccttggccccggggggggggggtttccccccgctcgggggctttaacccccacccccctcgggggtaaaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGG < NB501016:282:H2V7GAFX2:1:21103:13157:1849/54‑1 (MQ=60)
cccccgcccgggttgtggggtgcacatccccccgtgccggggcggtttaccccgctaaggcgtagtaaacccccccaccgcatggggggtaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCG < NB501016:282:H2V7GAFX2:3:11608:17641:14046/56‑1 (MQ=60)
ggtggtggggggcaacttgcccccgggtgggggggtgtacccccgctatgggtttttaacccccccccccctttgggggttaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAG < NB501016:282:H2V7GAFX2:4:11607:9779:17927/65‑1 (MQ=60)
gggcacttggccccctgtcggcggggtttaccccgctaaggcgctgttaaccccaccacccaatgcggggtaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCG < NB501016:282:H2V7GAFX2:4:11507:18103:7795/75‑1 (MQ=60)
atttgccccttttcggcgggttttaccccgctatggcggctgtatccccccaatcccttggggggtaaaacCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGG < NB501016:282:H2V7GAFX2:3:21610:14632:16741/80‑1 (MQ=60)
ccccctggcggcgggggtaaccccgctctgtgggtagtacacccccccccccattgggctaaaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGG < NB501016:282:H2V7GAFX2:1:21308:19092:7933/84‑1 (MQ=60)
gggtttacccggctatggggttgtattccccccaagctttttcggtttaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTG < NB501016:282:H2V7GAFX2:4:21502:1841:7279/98‑1 (MQ=60)
GGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCG > NB501016:282:H2V7GAFX2:3:21402:17260:3450/1‑151 (MQ=60)
ttaccggggtttggggttggtatagccacctagcatttctgggtaaaacCCGCTCCTACACAAAGCCCGCCTCCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGG < NB501016:282:H2V7GAFX2:1:21204:26384:11197/101‑1 (MQ=60)
CCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCC > NB501016:282:H2V7GAFX2:2:21308:12822:9227/1‑151 (MQ=60)
ccgcgaatgcgatggtggctatacctcCGCATTCGCGGGTAAACCCGCGCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCG > NB501016:282:H2V7GAFX2:1:11204:10299:17750/28‑151 (MQ=60)
CGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGG > NB501016:282:H2V7GAFX2:3:11505:9929:3460/1‑97 (MQ=60)
CGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTG > NB501016:282:H2V7GAFX2:4:11510:22260:9273/1‑151 (MQ=60)
GTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTG > NB501016:282:H2V7GAFX2:1:21102:16254:7127/1‑146 (MQ=60)
GCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGC > NB501016:282:H2V7GAFX2:2:11309:23100:15127/1‑110 (MQ=60)
CCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCC > NB501016:282:H2V7GAFX2:1:21309:16861:3588/1‑151 (MQ=60)
cccatgcgggtttaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGG < NB501016:282:H2V7GAFX2:3:21410:25612:15756/133‑1 (MQ=60)
gggggggtaaaccCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCC < NB501016:282:H2V7GAFX2:4:21512:17851:12500/138‑1 (MQ=60)
AAC‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACTTGCC > NB501016:282:H2V7GAFX2:3:21408:4186:9418/1‑151 (MQ=60)
C‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACTTGCCTT > NB501016:282:H2V7GAFX2:1:11205:8929:3174/1‑151 (MQ=60)
|
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACTTGCCTT > NC_002947_CJ‑RC/1126498‑1126795
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
