Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F25 I1 R1
|
61 |
74.4 |
3161000 |
98.6% |
3116746 |
146.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
1,126,647 |
+C |
intergenic (‑101/+48) |
tdcG‑II ← / ← gcvP‑I |
L‑serine dehydratase/glycine dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 1,126,645 | 1 | . | C | 100.0%
| 61.5
/ NA
| 18 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (13/5); total (13/5) |
GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACTT > NC_002947_CJ‑RC/1126500‑1126790
|
gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑cc < 2:136744/148‑1 (MQ=255)
ttccgatgcctatCTTTAACATGTCGAAGACGCTCAGTGACATGTGGACCTCTTGCCAGGTTGTTGGGTGGAAATTGATCCATGTAGTCGGGGTTTTTCTCGCGAATGCGTTGGTATAGCCACTATGGCATTCGCGGGTAAA‑cccgctc < 2:257300/147‑6 (MQ=255)
ctatCTTTAACCGGCCCAAGACGCTCTTTTAAATGTGCACCTCCTGCCAGGTTGTTGGGTGCACTTTGAGCCGTGTCGGCGGGGTTTAACCCGCTATTGCGGTGTTATTGCCACCATCGCTTTCGGGGTAAAACCC‑GCTCCTACACaga < 6:367262/147‑1 (MQ=255)
tcgaccaCGCTCTGTTACTTGTGCCCCTCCTGCCCGTTTGTGGGGTGCACTTTGCCCCCTGTCGGGGGGGGTTACCCCGCTATGGGGTTGTTAACCCCACCACCCCTTTGGGGGTAAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAg < 8:389183/143‑1 (MQ=255)
cccctggTTGGGGGGTGGACATTGCGCCCTGACGGCGGGGGTTACCCCGCTATTGCGCCGTAATCCCCACCACCCCATGCGGGGTAAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTcc < 8:286042/144‑1 (MQ=255)
gcgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAgg > 2:234208/1‑149 (MQ=255)
gTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCCAGCCAGGTGGCGGGGCGGGCTATCACGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACCCCAGGTTGc > 1:371868/1‑149 (MQ=38)
ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 1:77772/1‑149 (MQ=255)
ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 4:124548/1‑149 (MQ=255)
gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc > 2:227820/1‑148 (MQ=255)
ggAGGTATAGCCACCATCGCATTCGCGGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCCAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt > 3:219454/1‑149 (MQ=255)
taGCCACCATCGCATTCGCGGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAcc > 4:330704/1‑149 (MQ=255)
caTCGCATTCGCGGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGa > 1:329447/1‑149 (MQ=255)
aTCGCATTCGCGGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc > 4:86079/1‑149 (MQ=255)
gcgGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg > 2:75022/1‑134 (MQ=255)
gcgGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg < 1:75022/134‑1 (MQ=255)
gcgGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc > 1:387541/1‑147 (MQ=255)
gggTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 3:260506/145‑1 (MQ=255)
gggTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc > 4:260506/1‑145 (MQ=255)
gTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAc > 2:259705/1‑149 (MQ=255)
aaa‑CCGGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACtt < 8:16841/149‑1 (MQ=255)
|
GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTACTT > NC_002947_CJ‑RC/1126500‑1126790
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
