Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F14 I0 R1
|
248 |
61.6 |
2601574 |
98.7% |
2567753 |
146.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
798,664 |
T→G |
22.4% |
intergenic (+19/+8) |
fklB‑I → / ← PP_0685 |
FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 798,664 | 0 | T | G | 22.4%
| 54.7
/ 3.4
| 34 | intergenic (+19/+8) | fklB‑I/PP_0685 | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (19/7); new base G (4/4); total (23/11) |
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCCGCTCATGTCCAGGTCACCCTGGTCACGCAGT > NC_002947_CJ‑RC/798516‑798807
|
gCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCt < 5:252582/149‑1 (MQ=255)
tgatgaACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGc > 1:194218/1‑149 (MQ=255)
aaCGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑c < 3:17193/148‑1 (MQ=255)
aCGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCt < 6:257115/149‑1 (MQ=255)
aCGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCt < 6:215680/149‑1 (MQ=255)
cGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCAGGGCCGG‑CCTc < 5:285414/149‑1 (MQ=255)
tGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAAt > 5:36151/1‑149 (MQ=255)
gcagcaAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTc > 5:55860/1‑149 (MQ=255)
agcaAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCa > 2:219097/1‑149 (MQ=255)
cgcCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCGGCCGG‑CCTCAATTCCAGTCTGTccc > 4:158636/1‑149 (MQ=255)
gTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGgcg > 1:21326/1‑149 (MQ=255)
gTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCGCAAATACAGTGTGTCCCCCCAGGgcg > 5:197706/1‑149 (MQ=255)
gCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCAGT‑CCCCCACGGCGCaa > 6:52739/1‑149 (MQ=255)
aaGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACg > 3:191229/1‑149 (MQ=255)
aGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCGCAATTCCAGTCTGTCCCCCCAGGGCGCAACGc > 5:289062/1‑149 (MQ=255)
ggCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCGGCCGG‑CCGCAATTGCAGTCTGTCCCCCCCCGGCGCAACGcccccccc > 3:101774/1‑143 (MQ=255)
ttACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCGGCCGG‑GCGCAATTGCCGGCTGGGCCCCCCCGGCGCCACGCCCGCGCCTAGc > 3:10986/1‑149 (MQ=255)
aCGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCATGCAGGCCGCCCCTGCCGG‑CCTCAATTCAAGACTGGCCCCCCAGGCCCCAACCCCCCCGCaaac > 8:59782/1‑143 (MQ=255)
cAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGaacagaaa > 2:20327/1‑149 (MQ=255)
cAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCGCAATTACAGTCTGTCCCCCCAGGGCGCAACGCCCCCGCATAGcagaacagaa > 5:200457/1‑148 (MQ=255)
cgccgcACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑GCGCAATTACAGTCTGTCCCCCCCGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGttac > 5:212829/1‑147 (MQ=255)
gccccACCGGGTTCTGGTGTTCCCCGGCGCGCTGCTCTACGTTCTTAAATCCCCTGGCGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCt < 8:272186/149‑1 (MQ=255)
cacaGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCGGCCGG‑CCTCAATTACAGTCTGTGCCCCCCGGGCGCAACCCCCGCGCAAAGCAGAACACAAACAGATTACGTACAAGTTCCTTGAg > 4:299334/1‑149 (MQ=255)
ttCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCCATTACCGTCTGTCCCCCCCGGGCGCACCGCCCCCGCCGAGCCGAACAGAAAAACATTCCGGACCAGTGGCTTGAGCcccccc > 1:191508/1‑149 (MQ=255)
gtgtTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGtt > 6:27218/1‑148 (MQ=255)
aaCGTCGAGCAGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCg > 5:299567/2‑148 (MQ=255)
cGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAAt < 2:194218/149‑1 (MQ=255)
gAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAg > 4:81021/1‑149 (MQ=255)
ctgctCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGGTCCTTGAGCACCCCCGGGTCACTCGTATTAAGccc > 7:316100/1‑149 (MQ=255)
tAATGCCCTGGCGGGCGGCCGGGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAGCGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCCGCTCATGTCCAg < 3:279678/146‑1 (MQ=255)
tAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCCGCTCATGTCCAg < 6:299567/146‑1 (MQ=255)
tAATCCCGTGGCGGCCGGCCCGGCGGG‑CCGCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCCGCTCATGTCCAg < 6:36151/146‑1 (MQ=255)
cccTGCCGGCCGGCCCTGCGGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCCGCTCATGTCCAGGTCACCCt < 6:55860/149‑1 (MQ=255)
gggcggccCGGGCGGCCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCCGCTCATGTCCAGGTCACCCTGGTCAc < 4:172666/146‑1 (MQ=255)
ggccCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCCGCTCATGTCCAGGTCACCCTGGTCACGCAGt > 1:137838/1‑149 (MQ=255)
|
GCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGG‑CCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCCGCTCATGTCCAGGTCACCCTGGTCACGCAGT > NC_002947_CJ‑RC/798516‑798807
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
