Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F13 I0 R1 140 52.5 2164708 98.8% 2138731 146.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
JC NC_002947_CJ‑RC 1,311,719 Δ84 bp 22.4% intergenic (‑187/+8) PP_1144 ← / ← rapA membrane protein/RNA polymerase‑associated protein RapA

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947_CJ‑RC = 131171812 (0.260)5 (0.110) 5/260 NT 22.4% intergenic (‑186/+92) PP_1144/rapA membrane protein/RNA polymerase‑associated protein RapA
?NC_002947_CJ‑RC 1311803 = 23 (0.510)intergenic (‑271/+7) PP_1144/rapA membrane protein/RNA polymerase‑associated protein RapA

ACAACCCTGTGCAGACTGTCCAGAGGCAGTATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947_CJ‑RC/1311570‑1311718
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgggGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCGCGCCGCTTGCGCAGGGCGTCGATTTCGCTGTCACGCACACTTGGGTTGACCGCTTGCAATGCCA  >  NC_002947_CJ‑RC/1311803‑1311946
                                                                                                                                                                                                                                                                                                     
ACAACCCTGTGCAGACTGTCCAGAGGCAGTATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCG                                                                                                                                                    >  3:7379/1‑147
ACAACCCTGTGCAGACTGTCCAGAGGCAGTATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCG                                                                                                                                                    >  7:40296/1‑147
  AACCCTGTGCAGACTGTCCAGAGGCGGTATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCG                                                                                                                                                    <  7:156682/145‑1
  AACCCTGTGCAGACTGTCCAGAGGCGGTATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCG                                                                                                                                                    >  8:156682/1‑145
  AACCCTGTGCAGACTGTCCAGAGGCAGTATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTTGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGG                                                                                                                                                  <  7:265449/147‑1
  AACCCTGTGCAGACTGTCCAGAGGCAGTATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCG                                                                                                                                                    <  1:103435/145‑1
                               TAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGGCCTGCGCTAGCCAGCCA                                                                                                                                >  3:159373/1‑136
                               TAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGGCCTGCGCTAGCCAGCCA                                                                                                                                <  4:159373/136‑1
                                                                                       TTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCGCGCCG                                                             >  8:225967/1‑147
                                                                                                 AATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCGCGCCGCTTGCGCAG                                                    <  4:7379/146‑1
                                                                                                                                    CTTCCTGTGGGAGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTCCTCCAGCATTGCCAGGCCATCTTCGCGCCGCTTGCGCAGGGCGTCGATTTCGCTGTCACGCACACTTGGGTTGAC                >  7:245123/1‑147
                                                                                                                                                   GGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCGCGCCGCTTGCGCAGGGCGTCGATTTCGCTGTCACGCACACTTGGGTTGACCGCTTGCAATGCCA  >  8:44812/1‑146
                                                                                                                                                                                                                                                                                                     
ACAACCCTGTGCAGACTGTCCAGAGGCAGTATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947_CJ‑RC/1311570‑1311718
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgggGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCGCGCCGCTTGCGCAGGGCGTCGATTTCGCTGTCACGCACACTTGGGTTGACCGCTTGCAATGCCA  >  NC_002947_CJ‑RC/1311803‑1311946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A