Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F13 I0 R1
|
140 |
52.5 |
2164708 |
98.8% |
2138731 |
146.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
1,419,548:1 |
+C |
100% |
intergenic (‑37/+99) |
PP_1242 ← / ← PP_1244 |
hypothetical protein/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 1,419,548 | 1 | . | C | 93.3%
| 41.5
/ ‑2.5
| 15 | intergenic (‑37/+99) | PP_1242/PP_1244 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (1/13); total (1/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑C‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCC > NC_002947_CJ‑RC/1419409‑1419627
|
ggATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTc < 6:260397/147‑1 (MQ=255)
ggATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTc > 7:81978/1‑147 (MQ=255)
tGGTATAAGCAGGTCTTGCCGACGTTGGTGGTTTCTGTTGTTTTGTCGGTGGGAATTGTTTTGTTTCCCTGGATTCTGGGTTGCATGGGCTGG‑CATCTTAGCGGTCACTCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgt < 7:43902/147‑1 (MQ=255)
gTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgt < 2:168751/145‑1 (MQ=255)
gTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgt < 3:161783/145‑1 (MQ=255)
gTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgt < 5:100806/145‑1 (MQ=255)
ttgAAGCAGGTCTTGCGGACTGTGGTGGGTTGTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCGTGGGCTGGCC‑TCTTCGCGGGCTCGCCCGGTCCCACAGGTACTGTGCAGTGCGTGACGGCTgtgg < 3:174816/144‑1 (MQ=255)
aaGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCTTTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAc < 2:116364/147‑1 (MQ=255)
gTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtgtg < 8:82402/147‑1 (MQ=255)
ccGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGGTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAg < 6:205771/145‑1 (MQ=255)
cGACTGTGGTGGGTGCTGTTGTGTGGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGGTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGGGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCgg < 1:159291/147‑1 (MQ=38)
gACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCggg < 8:79890/147‑1 (MQ=255)
gACTGTGGTGGGTTCTGTTGTGTGGTCGGTGGGCATTGTTGTGTTTCCCTGGATTCTGGGTGGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCggg < 8:125229/147‑1 (MQ=38)
ggtggGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGcc < 5:191117/147‑1 (MQ=255)
ggggggTTCTGGTGTGTTGTCGGGGGGCATTGTTTTGTTACCCTGGATTCTGGGTTCCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCGGTGGTGACCCTGTGGGAGCGGGCGTg < 5:34414/142‑1 (MQ=37)
|
GGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑C‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCC > NC_002947_CJ‑RC/1419409‑1419627
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
