Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F13 I0 R1 140 52.5 2164708 98.8% 2138731 146.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
JC NC_002947_CJ‑RC 1,930,392 Δ63 bp 24.3% [apeB] [apeB]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947_CJ‑RC = 19303916 (0.130)3 (0.070) 3/258 NT 24.3% intergenic (‑58/+47) PP_1729/apeB hypothetical protein/M18 family aminopeptidase
?NC_002947_CJ‑RC 1930455 = 13 (0.290)coding (1273/1290 nt) apeB M18 family aminopeptidase

TGGTCAGCAGCATCAGGAACAGGAACACCGGGACGAACAGTGACAGGAACGGGGACATGGACAGCAATCCTTTTGCAGTGGGGCCATGTAACAAGCATAGCGCTTGACCAACGAGGCGTGATTGACCTTGAGCACGTTTAAGCAGGCGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947_CJ‑RC/1930240‑1930391
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcggcTACGGTAGAACGCGGTCAGTACCTTCACCAGGTGCGCCAGGTCATGGCTGCCACACAGCTCGCGAATGGAGTGCATGGCAAAGGTCGGCAAACCGATATCCACCGTGCGCACACCCAGGTGGCTGGCGGTGATCGGCCCGATG  >  NC_002947_CJ‑RC/1930455‑1930597
                                                                                                                                                                                                                                                                                                       
TGGTCAGCAGCATCAGGAACAGGAACACCGGGACGAACAGTGACAGGAACGGGGACATGGACAGCAATCCTTTTGCAGTGGGGCCATGTAACAAGCATAGCGCTTGACCAACGAGGCGTGATTGACCTTGAGCACGTTTAAGCAGGC                                                                                                                                                      >  7:31655/1‑147
    CAGCAGCATCAGGAACAGGAACACCGGGACGAACAGTGACAGGAACGGGGACATGGACAGCAATCCTTTTGCAGTGGGGCCATGTAACAAGCATAGCGCTTGACCAACGAGGCGTGATTGACCTTGAGCACGTTTAAGCAGGCGCGG                                                                                                                                                  <  7:54666/147‑1
        AGCATCAGGAACAGGAACACCGGGACGAACAGTGACAGGAACGGGGACATGGACAGCAATCCTTTTGCAGTGGGGCCATGTAACAAGCATAGCGCTTGACCAACGAGGCGTGATTGACCTTGAGCACGTTTAAGCAGGCGCGGCTAC                                                                                                                                              >  2:199743/1‑147
                    AGGAACACCGGGACGAACAGTGACAGGAACGGGGACATGGACAGCAATCCTTTTGCAGTGGGGCCATGTAACAAGCATAGCGCTTGACCAACGAGGCGTGATTGACCTTGAGCACGTTTAAGCAGGCGCGGCTACGGTAGAACGCGG                                                                                                                                  >  6:186175/1‑147
                                   AACAGTGACAGGAACGGGGACATGGACAGCAATCCTTTTGCAGTGGGGCCATGTAACAAGCATAGCGCTTGACCAACGAGGCGTGATTGACCTTGAGCACGTTTAAGCAGGCGCGGCTACGGTAGAACGCGGTCAGTACCTTCACCA                                                                                                                   <  5:186175/147‑1
                                                                             GTGGGGCCATGTAACAAGCATAGCGCTTGACCAACGAGGCGTGATTGACCTTGAGCACGTTTAAGCAGGCGCGGCTACGGTAGAACGCGGTCAGTACCTTCACCAGGTGCGCCAGGTCATGGCTGCCACACAGCTCGCGAATGGAGT                                                                         <  1:199743/147‑1
                                                                                                                                                    CGGCTACGGTAGAACGCGGTCAGTACCTTCACCAGGTGCGCCAGGTCATGGCTGCCACACAGCTCGCGAATGGAGTGCATGGCAAAGGTCGGCAAACCGATATCCACCGTGCGCACACCCAGGTGGCTGGCGGTGATCGGCCCGATG  >  2:63400/1‑147
                                                                                                                                                                                                                                                                                                       
TGGTCAGCAGCATCAGGAACAGGAACACCGGGACGAACAGTGACAGGAACGGGGACATGGACAGCAATCCTTTTGCAGTGGGGCCATGTAACAAGCATAGCGCTTGACCAACGAGGCGTGATTGACCTTGAGCACGTTTAAGCAGGCGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947_CJ‑RC/1930240‑1930391
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcggcTACGGTAGAACGCGGTCAGTACCTTCACCAGGTGCGCCAGGTCATGGCTGCCACACAGCTCGCGAATGGAGTGCATGGCAAAGGTCGGCAAACCGATATCCACCGTGCGCACACCCAGGTGGCTGGCGGTGATCGGCCCGATG  >  NC_002947_CJ‑RC/1930455‑1930597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A