Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F18 I1 R1 59 77.7 3515218 98.5% 3462489 145.9

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348668‑4348961
                                                                                                                                                |                                                                                                                                                     
CGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCC                                                                                                                                                   <  NB501016:282:H2V7GAFX2:3:21506:13761:10537/149‑1 (MQ=0)
 GGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACTGCTTCGGCTGACTTCTGCACGCTCGTCCCGTC                                                                                                                                                <  NB501016:282:H2V7GAFX2:2:21202:1667:9835/151‑1 (MQ=0)
 GGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTC                                                                                                                                                <  NB501016:282:H2V7GAFX2:2:11301:15058:9116/151‑1 (MQ=0)
      GACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTT                                                                                                                                           >  NB501016:282:H2V7GAFX2:3:11409:22918:18516/1‑151 (MQ=0)
      GACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCAGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTT                                                                                                                                           >  NB501016:282:H2V7GAFX2:1:11308:13934:12781/1‑151 (MQ=0)
        CAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTC                                                                                                                                         <  NB501016:282:H2V7GAFX2:3:21501:25282:8983/151‑1 (MQ=0)
        CAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTC                                                                                                                                         <  NB501016:282:H2V7GAFX2:3:21403:21258:6585/151‑1 (MQ=0)
         AGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCG                                                                                                                                        <  NB501016:282:H2V7GAFX2:2:21211:8851:1947/151‑1 (MQ=0)
          GTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCAT‑ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGAC                                                                                                                                      <  NB501016:282:H2V7GAFX2:3:21604:7480:5149/151‑1 (MQ=0)
                GCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCG                                                                                                                                 <  NB501016:282:H2V7GAFX2:3:11412:21411:3075/151‑1 (MQ=0)
                     GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGC                                                                                                                            >  NB501016:282:H2V7GAFX2:2:21202:10832:10635/1‑151 (MQ=0)
                            TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTG                                                                                                                      <  NB501016:282:H2V7GAFX2:2:11205:5149:18586/150‑1 (MQ=0)
                                    TACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGT                                                                                                              <  NB501016:282:H2V7GAFX2:2:21307:8033:8592/150‑1 (MQ=19)
                                     ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            <  NB501016:282:H2V7GAFX2:1:21107:5518:4014/151‑1 (MQ=21)
                                     ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            >  NB501016:282:H2V7GAFX2:1:21101:20226:17399/1‑151 (MQ=21)
                                     ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTA                                                                                                             <  NB501016:282:H2V7GAFX2:4:21503:24366:1941/150‑1 (MQ=21)
                                      CGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACA                                                                                                           <  NB501016:282:H2V7GAFX2:3:21506:9724:2903/151‑1 (MQ=22)
                                        GTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGA                                                                                                         <  NB501016:282:H2V7GAFX2:3:11601:8127:18349/151‑1 (MQ=21)
                                          TACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGAT                                                                                                        >  NB501016:282:H2V7GAFX2:2:11201:25841:8490/1‑150 (MQ=22)
                                          TACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTG                                                                                                                      <  NB501016:282:H2V7GAFX2:3:11408:23279:8060/136‑1 (MQ=0)
                                           ACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCT                                                                                                      >  NB501016:282:H2V7GAFX2:1:11101:6878:19721/1‑151 (MQ=21)
                                            CCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTC                                                                                                     >  NB501016:282:H2V7GAFX2:1:21203:20402:7929/1‑151 (MQ=22)
                                              AACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                   >  NB501016:282:H2V7GAFX2:3:21505:25031:5761/1‑151 (MQ=21)
                                              AACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                   >  NB501016:282:H2V7GAFX2:2:11106:11370:3767/1‑151 (MQ=21)
                                                CGAGACGGCGACGCCTACTGGCGGCATACCCGGTTCGGCCCTTGGTGGCGCGGTTAACCGCCCCTTACTACGGCTTCGGCTGACTTCTGCACGCGCATCCCGTCGCCGTTCGACGCGCGGTAGCACAGTGGCAAGCGTCCAGCTCTCCC                                                                                                   >  NB501016:282:H2V7GAFX2:4:21502:26376:20384/1‑149 (MQ=18)
                                                CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGCCGCGCGGTAG                                                                                                                             >  NB501016:282:H2V7GAFX2:2:21302:9188:3447/1‑123 (MQ=0)
                                                 GAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGG                                                                                                >  NB501016:282:H2V7GAFX2:3:21510:7737:10005/1‑151 (MQ=22)
                                                  AGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGG                                                                                               <  NB501016:282:H2V7GAFX2:4:11612:11660:3723/151‑1 (MQ=21)
                                                   GACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGT                                                                                              >  NB501016:282:H2V7GAFX2:1:21112:14492:9136/1‑151 (MQ=22)
                                                      GGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTC                                                                                                     <  NB501016:282:H2V7GAFX2:1:11308:8288:2004/141‑1 (MQ=22)
                                                           CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                                      <  NB501016:282:H2V7GAFX2:4:21510:23821:18565/151‑1 (MQ=21)
                                                           CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                                      >  NB501016:282:H2V7GAFX2:2:11102:6458:15120/1‑151 (MQ=21)
                                                           CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGTGTAATTCGCG                                                                                      >  NB501016:282:H2V7GAFX2:1:21201:12130:5458/1‑151 (MQ=21)
                                                           CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                                      <  NB501016:282:H2V7GAFX2:2:11211:10963:10846/151‑1 (MQ=22)
                                                                ACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACC                                                                                 <  NB501016:282:H2V7GAFX2:2:21110:20550:17702/151‑1 (MQ=21)
                                                                ACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGG                                                                                                <  NB501016:282:H2V7GAFX2:2:21201:14930:18913/136‑1 (MQ=22)
                                                                       GCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGC                                                                          <  NB501016:282:H2V7GAFX2:1:11211:21437:19031/151‑1 (MQ=22)
                                                                          TAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTT                                                                        >  NB501016:282:H2V7GAFX2:4:11502:9789:12179/1‑150 (MQ=22)
                                                                          TAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAG                                                                                                 <  NB501016:282:H2V7GAFX2:3:21407:19454:16487/125‑1 (MQ=23)
                                                                             CCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGC                                                                    >  NB501016:282:H2V7GAFX2:3:11601:20279:14999/1‑151 (MQ=22)
                                                                                GTTCGGCCTTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCC                                                                   <  NB501016:282:H2V7GAFX2:2:11106:3851:11686/149‑1 (MQ=21)
                                                                                GTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCC                                                                   <  NB501016:282:H2V7GAFX2:4:11608:25596:6712/149‑1 (MQ=21)
                                                                                   CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                              <  NB501016:282:H2V7GAFX2:4:11506:26793:17772/151‑1 (MQ=21)
                                                                                   CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                              >  NB501016:282:H2V7GAFX2:2:21109:5278:1180/1‑151 (MQ=22)
                                                                                    GGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGG                                                             >  NB501016:282:H2V7GAFX2:1:11311:12727:15261/1‑151 (MQ=21)
                                                                                     GCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                    >  NB501016:282:H2V7GAFX2:4:11406:4260:6276/1‑127 (MQ=22)
                                                                                      CCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGAT                                                           <  NB501016:282:H2V7GAFX2:3:11504:17664:5596/151‑1 (MQ=21)
                                                                                           GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                      <  NB501016:282:H2V7GAFX2:3:21502:17882:14500/151‑1 (MQ=21)
                                                                                           GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                      <  NB501016:282:H2V7GAFX2:4:11408:16738:16666/151‑1 (MQ=22)
                                                                                                CGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCAC                                                  >  NB501016:282:H2V7GAFX2:3:21601:17297:15079/1‑150 (MQ=21)
                                                                                                  CGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGC                                               >  NB501016:282:H2V7GAFX2:2:21309:24417:10544/1‑151 (MQ=22)
                                                                                                   GGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCG                                              >  NB501016:282:H2V7GAFX2:2:11301:20493:3223/1‑151 (MQ=21)
                                                                                                      TAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGC                                               <  NB501016:282:H2V7GAFX2:2:11308:11398:11705/147‑1 (MQ=22)
                                                                                                        ACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                         <  NB501016:282:H2V7GAFX2:1:11208:10036:19087/151‑1 (MQ=22)
                                                                                                         CCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGT                                        <  NB501016:282:H2V7GAFX2:4:11501:12825:11609/151‑1 (MQ=21)
                                                                                                           GCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGC                                      >  NB501016:282:H2V7GAFX2:4:11512:8601:10623/1‑151 (MQ=21)
                                                                                                            CCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGC                                      >  NB501016:282:H2V7GAFX2:3:21401:20061:16172/1‑150 (MQ=22)
                                                                                                              ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACTGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                   <  NB501016:282:H2V7GAFX2:3:21401:15875:15002/151‑1 (MQ=20)
                                                                                                              ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                   <  NB501016:282:H2V7GAFX2:1:21210:19835:17069/151‑1 (MQ=21)
                                                                                                                   CTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTG                              >  NB501016:282:H2V7GAFX2:3:21503:17341:11116/1‑151 (MQ=22)
                                                                                                                                  ACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG               <  NB501016:282:H2V7GAFX2:2:21104:8513:20006/151‑1 (MQ=21)
                                                                                                                                  ACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG               <  NB501016:282:H2V7GAFX2:2:21309:3767:2398/151‑1 (MQ=22)
                                                                                                                                  ACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG               >  NB501016:282:H2V7GAFX2:3:11609:10883:9197/1‑151 (MQ=22)
                                                                                                                                   CTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATT                 <  NB501016:282:H2V7GAFX2:2:11305:8012:10270/148‑1 (MQ=22)
                                                                                                                                      GTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCC           <  NB501016:282:H2V7GAFX2:2:21202:13203:17594/151‑1 (MQ=21)
                                                                                                                                        GCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAC         >  NB501016:282:H2V7GAFX2:2:11309:7559:12696/1‑151 (MQ=22)
                                                                                                                                        GCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAC         >  NB501016:282:H2V7GAFX2:3:21505:10949:18967/1‑151 (MQ=22)
                                                                                                                                         CACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC        <  NB501016:282:H2V7GAFX2:4:11510:2664:10531/151‑1 (MQ=21)
                                                                                                                                         CACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC        <  NB501016:282:H2V7GAFX2:2:11301:10978:19926/151‑1 (MQ=22)
                                                                                                                                           CGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAG                                                                                                          <  NB501016:282:H2V7GAFX2:1:21304:12616:13815/51‑1 (MQ=32)
                                                                                                                                             CTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC    >  NB501016:282:H2V7GAFX2:3:21609:22854:3025/1‑151 (MQ=21)
                                                                                                                                               CGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NB501016:282:H2V7GAFX2:3:21411:14927:13366/1‑151 (MQ=21)
                                                                                                                                               CGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC    <  NB501016:282:H2V7GAFX2:3:11511:10903:17236/149‑1 (MQ=21)
                                                                                                                                               CATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NB501016:282:H2V7GAFX2:3:21411:15484:17823/1‑151 (MQ=22)
                                                                                                                                                |                                                                                                                                                     
CGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348668‑4348961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑