Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F12 I1 R1 58 76.7 3263832 98.3% 3208346 146.5

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC  >  NC_002947_CJ‑RC/4348665‑4348959
                                                                                                                                                   |                                                                                                                                                   
ATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCC                                                                                                                                                  >  NB501016:282:H2V7GAFX2:2:21307:12080:19663/1‑151 (MQ=0)
       ATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCC                                                                                                                                           <  NB501016:282:H2V7GAFX2:1:11311:6794:18971/151‑1 (MQ=0)
             GTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCACCTTTCG                                                                                                                                      >  NB501016:282:H2V7GAFX2:1:21211:18512:14229/1‑150 (MQ=0)
                TCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGC                                                                                                                                  >  NB501016:282:H2V7GAFX2:3:11408:17130:16708/1‑151 (MQ=0)
                  CGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGC                                                                                                                                >  NB501016:282:H2V7GAFX2:4:11412:4663:2827/1‑151 (MQ=0)
                        GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGC                                                                                                                          >  NB501016:282:H2V7GAFX2:1:21111:17153:12629/1‑151 (MQ=0)
                          GAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCAC                                                                                                                        <  NB501016:282:H2V7GAFX2:1:21109:18180:1325/151‑1 (MQ=0)
                               TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTG                                                                                                                    <  NB501016:282:H2V7GAFX2:4:11501:23623:1782/150‑1 (MQ=0)
                                 CTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC                                                                                                                  >  NB501016:282:H2V7GAFX2:4:21411:9617:9382/1‑150 (MQ=2)
                                     ATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCG                                                                                                             <  NB501016:282:H2V7GAFX2:3:21409:21004:8458/151‑1 (MQ=17)
                                               CCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTC                                                                                                   >  NB501016:282:H2V7GAFX2:3:21403:11441:12146/1‑151 (MQ=22)
                                                   CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                 >  NB501016:282:H2V7GAFX2:4:11601:5725:6915/1‑149 (MQ=21)
                                                      GACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCTACGCGCGGTATAACAGTGGCAAGCGTACAGATCTTCCAGGGT                                                                                            <  NB501016:282:H2V7GAFX2:2:21211:8526:14117/151‑1 (MQ=19)
                                                      GACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGT                                                                                            <  NB501016:282:H2V7GAFX2:4:21402:1948:4362/151‑1 (MQ=22)
                                                                CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                  <  NB501016:282:H2V7GAFX2:3:11511:17751:18413/151‑1 (MQ=21)
                                                                CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                  >  NB501016:282:H2V7GAFX2:2:11209:21554:12185/1‑151 (MQ=22)
                                                                      GGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTT                                                                             >  NB501016:282:H2V7GAFX2:4:11602:6229:4310/1‑150 (MQ=22)
                                                                       GCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCC                                                                           >  NB501016:282:H2V7GAFX2:2:11306:14933:16988/1‑151 (MQ=21)
                                                                         GGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTG                                                                         <  NB501016:282:H2V7GAFX2:2:21310:1847:2633/151‑1 (MQ=21)
                                                                           CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                       >  NB501016:282:H2V7GAFX2:4:21407:18567:9248/1‑151 (MQ=21)
                                                                           CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                       <  NB501016:282:H2V7GAFX2:1:21308:2993:9578/151‑1 (MQ=21)
                                                                           CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                       <  NB501016:282:H2V7GAFX2:2:11310:9147:12550/151‑1 (MQ=22)
                                                                               tgaggatcaacgaccgcttcttccgcctggccgatataACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTAT                                                                    <  NB501016:282:H2V7GAFX2:3:21503:4675:12388/112‑1 (MQ=23)
                                                                               ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                   >  NB501016:282:H2V7GAFX2:1:11211:22776:14268/1‑151 (MQ=22)
                                                                                GCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTAGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGT                                                                  >  NB501016:282:H2V7GAFX2:2:11202:5990:4163/1‑151 (MQ=20)
                                                                                  TGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGA                                                                                                       <  NB501016:282:H2V7GAFX2:1:21312:21431:15166/112‑1 (MQ=24)
                                                                                   GTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCGCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGTGTAATTCGCGCGACCTTCCTGCTTATGCCTG                                                               <  NB501016:282:H2V7GAFX2:4:11611:22359:10241/151‑1 (MQ=20)
                                                                                      CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                            <  NB501016:282:H2V7GAFX2:2:11112:19821:8146/151‑1 (MQ=21)
                                                                                      CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                            <  NB501016:282:H2V7GAFX2:1:21301:26158:4515/151‑1 (MQ=21)
                                                                                       GGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGG                                                           >  NB501016:282:H2V7GAFX2:4:21608:15285:5044/1‑151 (MQ=22)
                                                                                        GCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGG                                                           >  NB501016:282:H2V7GAFX2:4:11612:21006:11245/1‑150 (MQ=21)
                                                                                         CCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTATGCACGCTCATCCCGTCGCCTTTCGCCGCGCGGTAGCACAGTCGCAAGCCTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGAT                                                         >  NB501016:282:H2V7GAFX2:2:21308:12783:11203/1‑151 (MQ=19)
                                                                                          CCTTGGTGGCGCGGTTTACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATC                                                        <  NB501016:282:H2V7GAFX2:4:21501:11527:14142/151‑1 (MQ=21)
                                                                                          CCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATC                                                        <  NB501016:282:H2V7GAFX2:3:11412:24720:19852/151‑1 (MQ=22)
                                                                                           CTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCT                                                       >  NB501016:282:H2V7GAFX2:1:11209:15389:16035/1‑151 (MQ=22)
                                                                                             TGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCT                                                       <  NB501016:282:H2V7GAFX2:2:21210:1930:16667/149‑1 (MQ=21)
                                                                                              GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                    <  NB501016:282:H2V7GAFX2:2:21211:18602:3815/151‑1 (MQ=21)
                                                                                              GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                    <  NB501016:282:H2V7GAFX2:3:11508:23290:20121/151‑1 (MQ=21)
                                                                                                    GCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAG                                              <  NB501016:282:H2V7GAFX2:3:11610:7592:13327/151‑1 (MQ=21)
                                                                                                     CGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGC                                             <  NB501016:282:H2V7GAFX2:3:21409:12751:17747/151‑1 (MQ=21)
                                                                                                            CCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGT                                      >  NB501016:282:H2V7GAFX2:2:21310:8585:19078/1‑151 (MQ=21)
                                                                                                                 ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                 <  NB501016:282:H2V7GAFX2:2:21311:21493:18698/151‑1 (MQ=21)
                                                                                                                 ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                 <  NB501016:282:H2V7GAFX2:1:21110:6611:11036/151‑1 (MQ=22)
                                                                                                                 ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                 <  NB501016:282:H2V7GAFX2:1:21107:10701:18476/151‑1 (MQ=22)
                                                                                                                  CTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                       >  NB501016:282:H2V7GAFX2:2:11109:24260:17380/1‑144 (MQ=21)
                                                                                                                    TACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTAT                              >  NB501016:282:H2V7GAFX2:1:11301:20260:15576/1‑151 (MQ=21)
                                                                                                                      CTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTG                            >  NB501016:282:H2V7GAFX2:3:21409:13449:15024/1‑151 (MQ=21)
                                                                                                                      CTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAG                                              <  NB501016:282:H2V7GAFX2:4:21607:14501:8738/133‑1 (MQ=22)
                                                                                                                      CTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTG                            >  NB501016:282:H2V7GAFX2:1:21107:6240:9505/1‑151 (MQ=22)
                                                                                                                        ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGG                           <  NB501016:282:H2V7GAFX2:2:21305:17058:12738/150‑1 (MQ=21)
                                                                                                                        ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGG                           <  NB501016:282:H2V7GAFX2:3:11502:1718:12520/150‑1 (MQ=21)
                                                                                                                        ACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGG                           <  NB501016:282:H2V7GAFX2:1:21306:25046:15041/150‑1 (MQ=22)
                                                                                                                        ACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGG                           <  NB501016:282:H2V7GAFX2:1:21202:9759:17088/150‑1 (MQ=22)
                                                                                                                           GCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTT                       >  NB501016:282:H2V7GAFX2:3:11511:19150:11441/1‑151 (MQ=22)
                                                                                                                               CGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGC                   <  NB501016:282:H2V7GAFX2:2:11308:23331:4158/151‑1 (MQ=22)
                                                                                                                                GGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                  >  NB501016:282:H2V7GAFX2:4:21606:5472:13403/1‑151 (MQ=21)
                                                                                                                                 GCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGCCCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGG                 >  NB501016:282:H2V7GAFX2:1:21306:12485:2075/1‑151 (MQ=21)
                                                                                                                                 GCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                  >  NB501016:282:H2V7GAFX2:1:11107:1687:13438/1‑150 (MQ=22)
                                                                                                                                  CTGACTTCTGCACGCTCATCCCGTCCCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATATCCCACGGTAAATCGCGCGCCCTTCCTGCTTATGCCAGTCGGATCTACGTCACACCGTACCGACCAAGTATTGGGCTTTGGCGAT                >  NB501016:282:H2V7GAFX2:1:21204:17509:5212/1‑151 (MQ=16)
                                                                                                                                   TGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATT               >  NB501016:282:H2V7GAFX2:4:11406:8347:9380/1‑151 (MQ=22)
                                                                                                                                    GACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGTTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTTCAAGTATTGGGCTTTGGCGAT                <  NB501016:282:H2V7GAFX2:2:11210:5355:10896/149‑1 (MQ=20)
                                                                                                                                    GACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGAT                <  NB501016:282:H2V7GAFX2:1:21210:17326:6970/149‑1 (MQ=21)
                                                                                                                                     ACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG             >  NB501016:282:H2V7GAFX2:2:11309:18978:11144/1‑151 (MQ=21)
                                                                                                                                     ACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG             <  NB501016:282:H2V7GAFX2:4:21405:2834:1662/151‑1 (MQ=22)
                                                                                                                                     ACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG             <  NB501016:282:H2V7GAFX2:3:11502:26620:2792/151‑1 (MQ=22)
                                                                                                                                      CTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGT            >  NB501016:282:H2V7GAFX2:2:11307:19833:19185/1‑151 (MQ=22)
                                                                                                                                      CTTCTGCACGCTCATCCCGTCGCCTTTAGACGCGCGGTAGCACAGTGGCAAGCGTACATATCTCCCAGGGTTATTCGCGCGACCTTACTGCTTATGCCACTAGGATCTACGTCACAGCGTTCACACCATGTATTGGGCTTTGGCGAGTTGT            >  NB501016:282:H2V7GAFX2:3:21611:17933:5196/1‑151 (MQ=15)
                                                                                                                                           GCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTT‑CGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC      <  NB501016:282:H2V7GAFX2:3:21407:9676:17577/151‑1 (MQ=20)
                                                                                                                                             ACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCT     <  NB501016:282:H2V7GAFX2:2:21104:6366:5361/151‑1 (MQ=21)
                                                                                                                                                CTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCAGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC  >  NB501016:282:H2V7GAFX2:4:11603:18653:16523/1‑151 (MQ=21)
                                                                                                                                                CTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                  <  NB501016:282:H2V7GAFX2:2:11305:14716:14740/135‑1 (MQ=23)
                                                                                                                                                   |                                                                                                                                                   
ATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC  >  NC_002947_CJ‑RC/4348665‑4348959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑