Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F12 I1 R1 58 76.7 3263832 98.3% 3208346 146.5

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTAATTACAATTAATCATCGGCTCGTTTAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGTACATCGTGATCGCCCT  >  NC_002947_CJ‑RC/4767876‑4768160
                                                                                                                                       |                                                                                                                                                     
GCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAAT                                                                                                                                        >  NB501016:282:H2V7GAFX2:4:21406:2756:9503/1‑151 (MQ=60)
    AGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTG                                                                                                                                    <  NB501016:282:H2V7GAFX2:4:21611:21634:5079/151‑1 (MQ=60)
        CAGCTTCTGATCGCCTTACGTTGAACGAAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAG                                                                                                                <  NB501016:282:H2V7GAFX2:2:11307:3269:17714/151‑1 (MQ=56)
        CAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAG                                                                                                                <  NB501016:282:H2V7GAFX2:3:21508:1326:10264/151‑1 (MQ=60)
             TCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATT                                                                                                             <  NB501016:282:H2V7GAFX2:1:21203:16314:12366/149‑1 (MQ=60)
                GATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAAC                                                                                                                          <  NB501016:282:H2V7GAFX2:1:11301:12920:4463/149‑1 (MQ=60)
                   CGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTC                                                                                                                     <  NB501016:282:H2V7GAFX2:1:21205:8796:11927/151‑1 (MQ=60)
                   CGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACA                                                                                                     <  NB501016:282:H2V7GAFX2:1:21202:8048:12793/151‑1 (MQ=57)
                        GACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAG                                                                                                                <  NB501016:282:H2V7GAFX2:2:11205:5300:4642/151‑1 (MQ=60)
                        GACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAA                                                                                                                 <  NB501016:282:H2V7GAFX2:4:21606:7238:4750/150‑1 (MQ=60)
                           GTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGAT                                                                                             <  NB501016:282:H2V7GAFX2:4:21409:2142:10963/151‑1 (MQ=45)
                             TGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTA                                                                                                            >  NB501016:282:H2V7GAFX2:4:21505:11291:1791/1‑150 (MQ=52)
                               AACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCATGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAAC                                                                                                          <  NB501016:282:H2V7GAFX2:4:11607:16581:2776/150‑1 (MQ=46)
                                 CGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAG                                                                                                                >  NB501016:282:H2V7GAFX2:1:21308:21985:3282/1‑142 (MQ=45)
                                 CGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATA                                                                                       <  NB501016:282:H2V7GAFX2:1:11312:12697:12187/151‑1 (MQ=35)
                                 CGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCAT                                                                                        >  NB501016:282:H2V7GAFX2:4:21408:7060:4257/1‑150 (MQ=35)
                                         ACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTT                                                                                >  NB501016:282:H2V7GAFX2:1:11106:7243:14561/1‑150 (MQ=30)
                                         ACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTT                                                                                >  NB501016:282:H2V7GAFX2:1:11205:3258:18847/1‑150 (MQ=30)
                                                  AGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCG                                                                      <  NB501016:282:H2V7GAFX2:1:11106:16643:7971/151‑1 (MQ=32)
                                                   GGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATT                                                                                     >  NB501016:282:H2V7GAFX2:2:11312:20809:14394/1‑151 (MQ=5)
                                                               TGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTG                                                          <  NB501016:282:H2V7GAFX2:3:21502:12796:3286/150‑1 (MQ=30)
                                                                 AATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCG                                                       <  NB501016:282:H2V7GAFX2:3:21412:11342:18858/151‑1 (MQ=32)
                                                                   TTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCATTAGCACCATCGCGCGTGACACTGCCGG                                                      >  NB501016:282:H2V7GAFX2:3:11505:7108:4391/1‑150 (MQ=22)
                                                                             GTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGAT                                           >  NB501016:282:H2V7GAFX2:4:11402:4790:4954/1‑151 (MQ=43)
                                                                              TCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATT                                          >  NB501016:282:H2V7GAFX2:4:11510:15215:11948/1‑151 (MQ=45)
                                                                                 CTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGC                                                         <  NB501016:282:H2V7GAFX2:3:21612:14001:15358/149‑1 (MQ=26)
                                                                                   ATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGCGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCG                                      <  NB501016:282:H2V7GAFX2:2:11201:25744:20208/150‑1 (MQ=50)
                                                                                      GAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTT                                                  <  NB501016:282:H2V7GAFX2:3:11511:7225:8626/151‑1 (MQ=40)
                                                                                             GGTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTC                           <  NB501016:282:H2V7GAFX2:4:21602:20814:9925/151‑1 (MQ=60)
                                                                                              GTATGATTACTATTGACAATTAATCATCGGCTCGTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAATTGTGAGCGGATAAGAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCG                          <  NB501016:282:H2V7GAFX2:2:21201:5719:15219/151‑1 (MQ=60)
                                                                                                   ATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCG                                      <  NB501016:282:H2V7GAFX2:2:11112:6246:5504/150‑1 (MQ=60)
                                                                                                                   AATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACC                     <  NB501016:282:H2V7GAFX2:4:21602:21092:15308/151‑1 (MQ=60)
                                                                                                                               GTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCTCGGCCAGTGAGTCGATACCAGTACATCGTG          >  NB501016:282:H2V7GAFX2:3:11610:14874:3614/1‑150 (MQ=60)
                                                                                                                                      GTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGTACATCGTGATCGCCCT  >  NB501016:282:H2V7GAFX2:2:21109:9936:8686/1‑151 (MQ=60)
                                                                                                                                       |                                                                                                                                                     
GCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTAATTACAATTAATCATCGGCTCGTTTAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCAGTACATCGTGATCGCCCT  >  NC_002947_CJ‑RC/4767876‑4768160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑