Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F11 I4 R1 73 908.3 39027734 99.3% 38754539 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 4,586,030 2 bp→TC intergenic (+113/+101) PP_4061 CDS (conserved protein of unknown function) → / ← PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM14,586,0300CT99.6% 800.5 / NA 229intergenic (+113/+102)PP_4061 CDS (conserved protein of unknown function)/PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase)–/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (9/218);  minor base G (0/1);  total (10/219)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE015451_phaM14,586,0310TC98.7% 872.4 / ‑3.6 229intergenic (+114/+101)PP_4061 CDS (conserved protein of unknown function)/PP_4063 CDS (putative Long‑chain‑fatty‑acid‑CoA ligase)–/–
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (8/218);  total (10/219)
Fisher's exact test for biased strand distribution p-value = 5.05e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACC‑AGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCC  >  AE015451_phaM1/4585900‑4586116
                                                                                                                                  ||                                                                                        
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGTTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:5147863/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:11674892/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:16363617/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:3578133/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:4473869/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:6980443/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:9354394/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:10427638/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:11177551/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:11283124/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:11030933/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:11816361/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:14693338/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:15239826/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:1666824/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:5105306/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:5153891/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:7901211/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  2:8712919/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:11989265/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:14046632/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:11560753/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCACTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:1726846/137‑1 (MQ=255)
        gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCGGATCTGCGCTGCTCCGTTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCg                                                                             <  1:481524/137‑1 (MQ=255)
             cTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCt                                                                        <  1:12878374/137‑1 (MQ=255)
                  gggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑Ag                                                                  <  2:12432108/137‑1 (MQ=39)
                  gggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑Ag                                                                  <  2:13996754/137‑1 (MQ=255)
                       gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGA‑cc                                                            <  1:12393249/137‑2 (MQ=39)
                       gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGA‑cc                                                            <  1:150845/137‑2 (MQ=39)
                       gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGA‑cc                                                            <  1:5930025/137‑2 (MQ=39)
                        aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGA‑ccg                                                           <  2:15524731/137‑3 (MQ=38)
                        aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGA‑ccg                                                           <  1:367101/137‑3 (MQ=39)
                         ccACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGNTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGA‑ccgt                                                          <  1:15766464/137‑4 (MQ=38)
                          cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGA‑ccgtg                                                         <  1:2439389/137‑5 (MQ=38)
                            cGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGcc                                                        <  2:2178149/137‑1 (MQ=38)
                             ggCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCg                                                       <  2:3245452/137‑1 (MQ=38)
                               cACCGGCGTGAACAGCTGGTCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGc                                                     <  2:1987213/137‑1 (MQ=38)
                                aCCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGcc                                                    >  2:12758416/1‑137 (MQ=38)
                                aCCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGcc                                                    >  1:18350365/1‑137 (MQ=38)
                                    gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGa                                                <  2:16182943/137‑1 (MQ=38)
                                    gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGa                                                <  2:16029880/137‑1 (MQ=38)
                                     cGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGaa                                               <  2:4223198/137‑1 (MQ=38)
                                        gAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgc                                            <  1:4651443/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAGAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACTCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  2:18584990/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑ATGGACCGTGCCGGCCTTGAATgcg                                           <  2:9282942/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:1249979/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:18052642/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  2:13852447/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  2:17480120/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  2:8221450/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:17939341/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  2:246620/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:13527451/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  2:2570642/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  2:2753710/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  2:16958767/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:8440283/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:11691004/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:5572718/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:5550407/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:15912548/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:16040855/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcg                                           <  1:17009431/137‑1 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGACGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgtg                                           <  1:16201017/137‑3 (MQ=38)
                                         aaCAGCTGATCTGCGCTGCTCCATCGAAAGTTTGAGGAGGACCGGGTGCTTGGCAAGTTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑ATGGACCGTACCGGCCTTGAATgcg                                           <  2:11782704/137‑1 (MQ=37)
                                          aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcgg                                          <  1:13361205/137‑1 (MQ=38)
                                          aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcgg                                          <  1:16001660/137‑1 (MQ=38)
                                           cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcggc                                         <  1:19345172/137‑1 (MQ=38)
                                            aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcggcg                                        <  1:1876407/137‑1 (MQ=38)
                                            aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcggcg                                        <  2:6590620/137‑1 (MQ=38)
                                            aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcggcg                                        <  2:16496945/137‑1 (MQ=38)
                                              cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCt                                      <  2:1361752/137‑1 (MQ=38)
                                              cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCt                                      <  2:5853843/137‑1 (MQ=38)
                                              cTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCt                                      <  1:14868769/137‑1 (MQ=38)
                                              cTGAGCTGCGCTACTCCATTGAAAGGTTGAGGATCACCGGGTACGTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTTGCGGGTAAACCCGCTCCTACC‑ATGGACCGTGCCGGCCTTGAATGCGGCGCt                                      <  2:3941882/137‑1 (MQ=37)
                                               ttaTCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTGGAAGGCGgcgcgg                                     <  2:4597407/135‑3 (MQ=37)
                                               tGATCTGCGCTGCTCCATTGAAAGGTTTAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑ATGGACCGTGCCGGCCTTGAATGCGGCGCTg                                     <  2:9671274/137‑1 (MQ=38)
                                               tGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTg                                     <  1:3824808/137‑1 (MQ=38)
                                                   cTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGcccc                                 <  2:18424292/137‑1 (MQ=38)
                                                    tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑ATGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                <  2:18278688/137‑1 (MQ=38)
                                                    tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                <  2:9750241/137‑1 (MQ=38)
                                                    tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                <  2:15645242/137‑1 (MQ=38)
                                                    tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                <  1:18960738/137‑1 (MQ=38)
                                                    tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                <  1:3504712/137‑1 (MQ=38)
                                                    tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt                                <  1:8677588/137‑1 (MQ=38)
                                                    tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCATGCCGGCCTCGAATGCGGCGCTGCCCCt                                <  2:14604619/137‑1 (MQ=38)
                                                        ctgctCCATTGAAAGTTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                            <  2:14786664/137‑1 (MQ=38)
                                                        ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTACACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                            <  1:19128751/137‑1 (MQ=38)
                                                        ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                            <  1:9605382/137‑1 (MQ=38)
                                                        ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                            <  2:1225246/137‑1 (MQ=38)
                                                        ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                            <  2:6281913/137‑1 (MQ=38)
                                                        ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                            <  1:10608784/137‑1 (MQ=38)
                                                        ctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTa                            <  1:17400254/137‑1 (MQ=38)
                                                         tgctCCATTGAAAGGTTGGGGGTGGCAGGGTGCTTGGCGAGGGGAAGAGCTGGAATGGATCGGGTCGGCCTCTGCGCGGGTAAACCCGCTCCTACC‑GTGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGGAg                           <  1:2291774/137‑1 (MQ=255)
                                                         tgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                           <  1:3454311/137‑1 (MQ=38)
                                                              cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATgcggcg                                        <  2:5014335/119‑1 (MQ=37)
                                                              cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAACCGCTCCCCCCAAGGGA‑ACTGCCGGACCTGGACGCTGTGCTGTCCCCGGTAGGAgag                      >  2:5259717/1‑135 (MQ=255)
                                                               agtaaaaGGTGGGGGAGGACCGGGTGCTGGACAAGGTGAAGATCTTGCATTGATTGGGCTGTCCTCTTCGCGGGGAAACCCGCCCCTACC‑AGGGACCGTGCCGTCCTGGAATGCGACGCTGCCCCGGGTAGGAGCgg                     <  2:14357948/133‑1 (MQ=255)
                                                                        ttGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                           >  2:11569844/1‑122 (MQ=37)
                                                                        ttGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC‑AGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAg                           <  1:11569844/122‑1 (MQ=37)
                                                                                                                                  ||                                                                                        
CCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACC‑AGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCC  >  AE015451_phaM1/4585900‑4586116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  AE015451_phaM1/4585886‑4586183
                                                                                                                                                      |                                                                                                                                                     
CGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTTGC‑GGG                                                                                                                                                      >  LH00941:114:22FVMWLT1:2:1124:43176:7181/1‑151 (MQ=60)
       CCAGGAGCTACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGTTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1242:45200:25484/151‑1 (MQ=60)
            AGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCC                                                                                                                                           <  LH00941:114:22FVMWLT1:2:2246:33700:23210/151‑1 (MQ=60)
            AGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCC                                                                                                                                           <  LH00941:114:22FVMWLT1:2:2246:33690:23194/151‑1 (MQ=60)
               CACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAC                                                                                                                                        <  LH00941:114:22FVMWLT1:2:1112:41151:22537/151‑1 (MQ=60)
               CACCGGTGAAGGCTGAAGGGTAGACCACGGCACCTGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAGGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATGGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAC                                                                                                                                        <  LH00941:114:22FVMWLT1:2:2245:28753:19799/151‑1 (MQ=60)
                ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAATGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1122:44720:23242/151‑1 (MQ=60)
                ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1244:49379:17157/151‑1 (MQ=60)
                ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                                                                                       <  LH00941:114:22FVMWLT1:2:2170:31536:26284/151‑1 (MQ=60)
                ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1149:20562:12849/151‑1 (MQ=60)
                ACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACC                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1118:44202:7517/151‑1 (MQ=60)
                 CCGGTGAAGGCTTAATGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1246:36131:15155/151‑1 (MQ=60)
                 CCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1246:36113:15155/151‑1 (MQ=60)
                 CCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA                                                                                                                                      <  LH00941:114:22FVMWLT1:2:2236:49333:27485/151‑1 (MQ=60)
                   GGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGG                                                                                                                                    >  LH00941:114:22FVMWLT1:2:2221:20359:8078/1‑151 (MQ=60)
                   GGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGG                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1149:12871:16372/151‑1 (MQ=60)
                    GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:2163:44673:4106/151‑1 (MQ=60)
                    GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:2149:28966:17189/151‑1 (MQ=60)
                    GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:1214:2026:19879/151‑1 (MQ=60)
                    GTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:2154:36196:6717/151‑1 (MQ=60)
                     TGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA                                                                                                                                  <  LH00941:114:22FVMWLT1:2:1142:52162:11024/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGTGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTGGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1124:4975:14451/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGTTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1205:43647:6172/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2142:24880:5436/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2206:30824:9839/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1266:48131:2153/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1167:44100:14579/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1170:7851:2041/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2101:37786:1849/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1165:42436:12849/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1265:51644:1673/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1119:3330:5868/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2133:22837:12882/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1254:25508:7966/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2163:19499:2073/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1231:12214:14179/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2134:38692:12513/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2130:8747:8590/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2226:21228:12241/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2128:5955:12785/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1126:20627:29359/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1126:6390:4251/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2127:37370:7245/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2263:49037:13490/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2127:37351:7245/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1135:43915:5612/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1140:46476:23242/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:2119:5770:15700/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1152:34264:18390/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCACTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1126:6381:4235/151‑1 (MQ=60)
                      GAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCGGATCTGCGCTGCTCCGTTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGA‑C                                                                                                                                <  LH00941:114:22FVMWLT1:2:1108:46643:3258/151‑1 (MQ=60)
                           CTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGC                                                                                                                            <  LH00941:114:22FVMWLT1:2:1152:19841:6028/151‑1 (MQ=60)
                           CTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGC                                                                                                                            <  LH00941:114:22FVMWLT1:2:2146:49129:8590/151‑1 (MQ=60)
                                GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGctgtctcttata                                                                                                                       >  LH00941:114:22FVMWLT1:2:1133:16892:4090/1‑139 (MQ=60)
                                GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2222:3838:27886/151‑1 (MQ=60)
                                GGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCC                                                                                                                       >  LH00941:114:22FVMWLT1:2:2254:35844:17797/1‑151 (MQ=60)
                                   TAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTG                                                                                                                    <  LH00941:114:22FVMWLT1:2:2213:44655:2857/151‑1 (MQ=60)
                                     GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAA                                                                                                                  <  LH00941:114:22FVMWLT1:2:2139:28652:9215/151‑1 (MQ=60)
                                     GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAA                                                                                                                  <  LH00941:114:22FVMWLT1:2:2139:28670:9215/151‑1 (MQ=60)
                                     GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAA                                                                                                                  <  LH00941:114:22FVMWLT1:2:1103:32812:7645/151‑1 (MQ=60)
                                     GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAA                                                                                                                  <  LH00941:114:22FVMWLT1:2:1135:25499:27709/151‑1 (MQ=60)
                                     GACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAA                                                                                                                  <  LH00941:114:22FVMWLT1:2:1216:13148:14995/151‑1 (MQ=60)
                                      ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAATGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAAT                                                                                                                 <  LH00941:114:22FVMWLT1:2:1241:36639:17669/151‑1 (MQ=60)
                                      ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAAT                                                                                                                 <  LH00941:114:22FVMWLT1:2:1106:6658:12465/151‑1 (MQ=60)
                                      ACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAAT                                                                                                                 <  LH00941:114:22FVMWLT1:2:1106:6676:12465/151‑1 (MQ=60)
                                       CCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATG                                                                                                                <  LH00941:114:22FVMWLT1:2:2147:18630:5756/151‑1 (MQ=60)
                                       CCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGNTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATG                                                                                                                <  LH00941:114:22FVMWLT1:2:2217:34069:24811/151‑1 (MQ=60)
                                        CACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1136:50017:9999/151‑1 (MQ=60)
                                                 GGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCC                                                                                                      >  LH00941:114:22FVMWLT1:2:1167:44636:17301/1‑151 (MQ=60)
                                                  GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGNCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1154:26331:1160/151‑1 (MQ=60)
                                                  GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1235:23955:21384/151‑1 (MQ=60)
                                                  GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1128:27570:12113/151‑1 (MQ=60)
                                                  GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2122:21108:21544/151‑1 (MQ=60)
                                                  GCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2160:33552:28814/151‑1 (MQ=60)
                                                   CGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGgcagcgccgcattcaaggccggcacggtccc                                                                                                    <  LH00941:114:22FVMWLT1:2:2230:32590:18918/151‑32 (MQ=60)
                                                      GAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGG                                                                                                 <  LH00941:114:22FVMWLT1:2:1167:20387:28398/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTNATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2249:50581:16709/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2163:28097:7966/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGgcagcgccgcattcaaggccggcacggtccctggt                                                                                                <  LH00941:114:22FVMWLT1:2:2207:48168:15988/151‑36 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGTGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2224:26535:2729/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCTTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:1211:35336:1448/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2235:31351:18982/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2250:36732:11424/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2221:14119:23050/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2219:5752:27485/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2219:5724:27469/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2144:11891:16564/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2155:36575:18166/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2155:36584:18182/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2157:6251:11472/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2128:36196:5179/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:1119:17354:7966/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:1151:17484:7037/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:1153:42519:3162/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:1211:24436:10624/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:1211:24455:10624/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:1211:35354:1448/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2250:36741:11440/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:1252:48233:4026/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2129:18168:3930/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2248:14322:22729/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2115:18418:14259/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2248:14313:22713/151‑1 (MQ=60)
                                                       AACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGACGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGTGGCGCTGCCCCTGGT                                                                                                <  LH00941:114:22FVMWLT1:2:2224:26525:2713/151‑1 (MQ=60)
                                                        ACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTA                                                                                               <  LH00941:114:22FVMWLT1:2:2153:44507:6861/151‑1 (MQ=60)
                                                        ACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTA                                                                                               <  LH00941:114:22FVMWLT1:2:2221:24408:6957/151‑1 (MQ=60)
                                                         CAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAG                                                                                              <  LH00941:114:22FVMWLT1:2:2268:23243:18614/151‑1 (MQ=60)
                                                         CAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAG                                                                                              <  LH00941:114:22FVMWLT1:2:2268:23234:18630/151‑1 (MQ=60)
                                                         CAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAG                                                                                              <  LH00941:114:22FVMWLT1:2:1214:19749:5740/151‑1 (MQ=60)
                                                          AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                             <  LH00941:114:22FVMWLT1:2:2236:2368:11344/151‑1 (MQ=60)
                                                          AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                             <  LH00941:114:22FVMWLT1:2:2236:2378:11328/151‑1 (MQ=60)
                                                          AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                             <  LH00941:114:22FVMWLT1:2:1128:15006:8478/151‑1 (MQ=60)
                                                          AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                             <  LH00941:114:22FVMWLT1:2:2212:20359:6540/151‑1 (MQ=60)
                                                          AGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGG                                                                                             <  LH00941:114:22FVMWLT1:2:1259:30769:28190/151‑1 (MQ=60)
                                                            CTGATCTGCGCTGCTCCATTGAAATGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCAGTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCATGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                           <  LH00941:114:22FVMWLT1:2:1244:47632:20343/151‑1 (MQ=60)
                                                            CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                           <  LH00941:114:22FVMWLT1:2:2255:13046:10848/151‑1 (MQ=60)
                                                            CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                           <  LH00941:114:22FVMWLT1:2:2204:40929:25163/151‑1 (MQ=60)
                                                            CTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAG                                                                                           <  LH00941:114:22FVMWLT1:2:2251:3348:12273/151‑1 (MQ=60)
                                                             TGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGC                                                                                          <  LH00941:114:22FVMWLT1:2:1156:13194:5083/151‑1 (MQ=60)
                                                             TGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGC                                                                                          <  LH00941:114:22FVMWLT1:2:2230:19712:7853/151‑1 (MQ=60)
                                                              GATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGctgt                                                                                         >  LH00941:114:22FVMWLT1:2:1203:41391:7773/1‑147 (MQ=60)
                                                                 CTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGT                                                                                      <  LH00941:114:22FVMWLT1:2:2102:39968:21737/151‑1 (MQ=60)
                                                                 CTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGT                                                                                      <  LH00941:114:22FVMWLT1:2:1218:9672:9583/151‑1 (MQ=60)
                                                                  TGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTT                                                                                     <  LH00941:114:22FVMWLT1:2:1255:44775:14339/151‑1 (MQ=60)
                                                                  TGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTT                                                                                     <  LH00941:114:22FVMWLT1:2:2263:27764:6588/151‑1 (MQ=60)
                                                                  TGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTT                                                                                     <  LH00941:114:22FVMWLT1:2:1151:35900:16869/151‑1 (MQ=60)
                                                                      CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTACACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                 <  LH00941:114:22FVMWLT1:2:2265:40153:16869/151‑1 (MQ=60)
                                                                      CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                 <  LH00941:114:22FVMWLT1:2:2241:39801:5660/151‑1 (MQ=60)
                                                                      CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                 <  LH00941:114:22FVMWLT1:2:1106:20941:28622/151‑1 (MQ=60)
                                                                      CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                 <  LH00941:114:22FVMWLT1:2:2113:31703:10335/151‑1 (MQ=60)
                                                                      CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                 <  LH00941:114:22FVMWLT1:2:1132:51422:27517/151‑1 (MQ=60)
                                                                      CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                 <  LH00941:114:22FVMWLT1:2:2101:5364:24859/151‑1 (MQ=60)
                                                                      CTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACC                                                                                 <  LH00941:114:22FVMWLT1:2:1268:43601:17013/151‑1 (MQ=60)
                                                                       TGCTCCATTGAAAGGTTGGGGGTGGCAGGGTGCTTGGCGAGGGGAAGAGCTGGAATGGATCGGGTCGGCCTCTGCGCGGGTAAACCCGCTCCTACCGTGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGGAGGAGCGGGTTTACCC                                                                                <  LH00941:114:22FVMWLT1:2:1134:4005:6941/151‑1 (MQ=36)
                                                                       TGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC                                                                                <  LH00941:114:22FVMWLT1:2:2263:42103:8366/151‑1 (MQ=60)
                                                                       TGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC                                                                                <  LH00941:114:22FVMWLT1:2:1150:44451:24667/151‑1 (MQ=60)
                                                                       GGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCC                                                                                <  LH00941:114:22FVMWLT1:2:2127:2858:11008/151‑1 (MQ=60)
                                                                        GCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCG                                                                               <  LH00941:114:22FVMWLT1:2:1206:26183:23322/151‑1 (MQ=60)
                                                                                                                                                   cgGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGC    >  LH00941:114:22FVMWLT1:2:2211:7222:14627/3‑151 (MQ=60)
                                                                                                                                                     GGTAACCCCGCTCCTCCCAGGGACCGTGCCGGCCTTGCATGCGGCGTTGCCCCTCGTAGGCGCGGGTTTCCCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACCGACCTTAAGCATGCGG  >  LH00941:114:22FVMWLT1:2:1120:13851:22938/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:1118:44618:3146/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:2226:20267:19030/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:2255:9570:14947/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:1222:15247:2713/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:1230:18843:18966/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:1252:25925:20440/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:1252:25934:20424/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:2113:14026:2906/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:2124:42002:2585/1‑151 (MQ=60)
                                                                                                                                                     GGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCATGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  LH00941:114:22FVMWLT1:2:2153:1767:8382/1‑151 (MQ=60)
                                                                                                                                                      |                                                                                                                                                     
CGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGG  >  AE015451_phaM1/4585886‑4586183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑