Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F11 I3 R1 74 850.3 37733610 99.3% 37469474 136.1

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGGGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGATTCCCTTTAT  >  AE015451_phaM1/2586482‑2586637
                                                                                                                                                   |        
TGGGGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGA‑TCCCT      <  LH00941:114:22FVMWLT1:2:2262:19194:9295/151‑1 (MQ=60)
TGGGGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGA‑TCCCT      <  LH00941:114:22FVMWLT1:2:2262:50396:18886/151‑1 (MQ=60)
TGGGGGGCATCCGGGGCCGCGCCAGGGCGCTGGGGGAGACCACAGCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGAGTACTTAATGATGATTACTAAGAGA‑TCCCT      <  LH00941:114:22FVMWLT1:2:2248:41160:8206/151‑1 (MQ=60)
 GGGGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGA‑TCCCTT     <  LH00941:114:22FVMWLT1:2:2163:51764:22313/151‑1 (MQ=60)
  GGGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGA‑TCCCTTT    <  LH00941:114:22FVMWLT1:2:1265:37388:19159/151‑1 (MQ=60)
  GGGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGA‑TCCCTTT    <  LH00941:114:22FVMWLT1:2:1265:37398:19143/151‑1 (MQ=60)
  GGGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGA‑TCCCTTT    >  LH00941:114:22FVMWLT1:2:2207:30186:11873/1‑151 (MQ=60)
   GGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGATCCCTTTT    <  LH00941:114:22FVMWLT1:2:2104:37777:25820/151‑1 (MQ=60)
   GGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGATCCCTTTT    <  LH00941:114:22FVMWLT1:2:2128:20045:29118/151‑1 (MQ=60)
    GGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATTCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGA‑TCCCTTTTT  >  LH00941:114:22FVMWLT1:2:2121:43869:17189/1‑151 (MQ=60)
    GGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGA‑TCCCTTTTT  >  LH00941:114:22FVMWLT1:2:2128:25296:26524/1‑151 (MQ=60)
                                                                                                                                                   |        
TGGGGGGCATCCGGGGCCGCGCCAGGGCGCTGTGGGAGACCACATCCAGAGTCGCGGACCCACAATTGGATTCCTTAGCGATGCCCTCTGAGATGACCCTTAGAGACATCCTTAGAAATGATTACTTAATGATGATTACTAAGAGATTCCCTTTAT  >  AE015451_phaM1/2586482‑2586637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑