Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F11 I4 R1 74 758.2 33768536 99.3% 33532156 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
MC JC AE015451_phaM1 1,124,024 Δ5,919 bp [gcvT‑I CDS (aminomethyltransferase)]–[gcvH‑I CDS (glycine cleavage system H protein 1)] [gcvT‑I CDS (aminomethyltransferase)], tdcG‑II CDS (L‑serine dehydratase), gcvP‑I CDS (glycine dehydrogenase), [gcvH‑I CDS (glycine cleavage system H protein 1)]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ AE015451_phaM1 1124024 1129942 5919 725 [0] [0] 729 [gcvT‑I CDS (aminomethyltransferase)]–[gcvH‑I CDS (glycine cleavage system H protein 1)] [gcvT‑I CDS (aminomethyltransferase)],tdcG‑II CDS (L‑serine dehydratase),gcvP‑I CDS (glycine dehydrogenase),[gcvH‑I CDS (glycine cleavage system H protein 1)]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451_phaM1 = 11240230 (0.000)724 (0.960) 114/270 0.4 100% coding (1120/1122 nt) gcvT‑I CDS (aminomethyltransferase)
?AE015451_phaM1 1129943 = 0 (0.000)coding (3/384 nt) gcvH‑I CDS (glycine cleavage system H protein 1)

GATK/CNVnator alignment

N/A